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Platform GPL14917 Query DataSets for GPL14917
Status Public on Nov 22, 2011
Title US Army Northern bobwhite (Colinus virginianus) custom brain tissue cDNA microarray
Technology type spotted DNA/cDNA
Distribution non-commercial
Organism Colinus virginianus
Manufacturer US Army, Engineer Research and Development Center (ERDC)
Manufacture protocol Microarrays were constructed by spotting the Northern bobwhite cDNA library, ArrayControlâ„¢ Spot cDNAs (Ambion), and printing buffer blanks onto Ultra GAPS-coated microarray slides (Corning Inc., Corning, NY). Spotted cDNAs were immobilized on the chip surface by applying 300 mJ of UV radiation in a Stratageneâ„¢ UV Stratalinker 2400 (Agilent Technologies, Waldbronn, Germany). Each microarray included 2 technical replicates for each microarray target.
Coating Ultra GAPS
 
Description Northern bobwhite
 
Submission date Nov 22, 2011
Last update date Nov 22, 2011
Contact name Kurt A Gust
E-mail(s) kurt.a.gust@usace.army.mil
Phone 601-634-3593
Organization name US Army ERDC
Department Environmental Laboratory
Lab Environmental Genomics and Systems Biology Team
Street address 3909 Halls Ferry Rd.
City Vicksburg
State/province MS
ZIP/Postal code 39180
Country USA
 
Samples (110) GSM838389, GSM838390, GSM838391, GSM838392, GSM838393, GSM838394 
Series (8)
GSE27653 Neurotoxicogenomic investigations to assess mechanisms of action of the munitions constituents RDX and 2,6-DNT in Northern bobwhite (Colinus virginianus)
GSE33888 Neurotoxicogenomic investigations to assess mechanisms of action of the munitions constituents RDX and 2,6-DNT in Northern bobwhite (Colinus virginianus) (part 1 of 10)
GSE33893 Neurotoxicogenomic investigations to assess mechanisms of action of the munitions constituents RDX and 2,6-DNT in Northern bobwhite (Colinus virginianus) (part 2 of 10)

Data table header descriptions
ID
Clone Unique Clone ID
GI GenBank identifier
Accession Accession of BLAST match
Length Length of sequence read
bit Bit score
Evalue Evalue
Species Species associated with BLASTx match
Organism Common name of species associated with BLASTx match
Description Expressed sequence tag (EST) of probe
Function Functional information about EST
SPOT_ID

Data table
ID Clone GI Accession Length bit Evalue Species Organism Description Function SPOT_ID
1 b b
2 1L h5 1L h5
3 1J g1 1J g1
4 1F g9 197708614
5 1C h5 197708180 YP_025977.1 516 377 3E-35 Anseranas semipalmata cytochrome c oxidase subunit I
6 1A g1 197708498 AAT97269.1 129 77 1.6 Culicoides sonorensis Non-Significant Match
7 4K g9 4K g9
8 4H h5 4H h5
9 4F g1 4F g1
10 4C g9 4C g9
11 2U h5 2U h5
12 2S g1 2S g1
13 2N g9 209979432 ref|NM_204157.2| 363 3E-105 Gallus gallus Chicken eukaryotic translation elongation factor 1 alpha 1 (EEF1A1), mRNA. This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes.
14 2K h5 209979147 AAQ62756.1 183 252 8E-21 Eleginus gracilis S2 ribosomal protein
15 2I g1 209979066 CAG31600.1 236 478 6E-47 Gallus gallus Chicken hypothetical protein
16 b g9 b g9
17 2C h5 2C h5
18 2A g1 2A g1
19 b b
20 1L h9 1L h9

Total number of rows: 9216

Table truncated, full table size 531 Kbytes.




Download family Format
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