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Status
Public on Feb 16, 2012
Title
miRXplore TM Microarray (911)
Technology type
spotted DNA/cDNA
Distribution
commercial
Organisms
Homo sapiens ; Mus musculus ; Rattus norvegicus
Manufacturer
Miltenyi Biotec GmbH, Cologne, Germany
Manufacture protocol
1.2 nl of 10 µM HPLC purified, 5’ amino-modified DNA oligonucleotides (Metabion) were spotted four times on aminopropyltrimethoxysilane / phenylendiisothiocyanate coated glass slides using a customized piezoelectric spotter.
Description
The miRXplore TM Microarray is composed of 6228 DNA oligonucleotides with a sequence being reverse complement to the respective mature miRNA or control RNA, representing a total of 1460 miRNA sequences and 97 controls with all probes spotted in quadruplicates. The 1460 miRNA sequences covered sequence verified miRNAs of the species human (878) mouse (682) and rat (427) as well as viral miRNAs (141) as deposited in the miRBase sequence database version 14.0. The 97 positive and negative control probes were chosen as follows. Three sets of controls, miRControl 1 (four sequences used to assess efficiency and sensitivity of labelling), miRControl 2 (single sequence used to assess efficiency of small RNA enrichment) and miRControl 3 (43 sequences used to normalize signal intensities across different samples and arrays) were artificial sequences chosen not be present in any of the human, mouse, rat or viral genome. A fourth set of positive controls presented 36 positive small but non-miRNA sequences including probes e.g. for 5.1 sRNA, tRNAs, and U6 RNAs. A fifth set of oligonucleotides included 13 negative controls like different single or double mismatch, antisense, or scrambled miRNA sequences. Microarrays were manufactured by Miltenyi Biotec. Briefly, 1.2 nl of 10 µM HPLC purified, 5’ amino-modified DNA oligonucleotides (Metabion) were spotted on aminopropyltrimethoxysilane / phenylendiisothiocyanate coated glass slides in quadruplicates using a customized piezoelectric spotter.
Web link
http://www.miltenyibiotec.com
Contributor(s)
Gerstmayer B , Bosio A
Submission date
Feb 15, 2012
Last update date
Feb 16, 2012
Contact name
Silvia Rueberg
E-mail(s)
silvia@miltenyibiotec.de
Organization name
Miltenyi Biotec GmbH
Department
Genomic Services
Street address
Friedrich-Ebert-Str. 68
City
Bergisch Gladbach
ZIP/Postal code
51429
Country
Germany
Samples (4)
GSM877229 , GSM877230 , GSM877231 , GSM877232
Series (1)
GSE35901
MicroRNA expression analysis of tumor tropic mesenchymal stem cells
Data table header descriptions
ID
stem-loop_acc
Stem-loop_acc
mature_acc
Mature_acc
name
Name
miltenyi_id
cDNA identifier at Miltenyi Biotec GmbH
miRNA_ID_LIST
miRNA identifiers
SPOT_ID
Data table
ID
stem-loop_acc
mature_acc
name
miltenyi_id
miRNA_ID_LIST
SPOT_ID
1
MI0000558 , MI0000829 , MI0000063 , MI0005453 , MI0005351 , MI0001172
MIMAT0000522 , MIMAT0000775 , MIMAT0000063 , MIMAT0004331 , MIMAT0004162 , MIMAT0001102
LET-7B
35816
LET-7B
2
MI0000064 , MI0000559 , MI0000560 , MI0000830 , MI0000831 , MI0005454 , MI0002445 , MI0001174
MIMAT0000776 , MIMAT0000523 , MIMAT0000064 , MIMAT0004332 , MIMAT0002151 , MIMAT0001104
LET-7C
35817
LET-7C
3
MI0000103 , MI0000739 , MI0000148 , MI0000886 , MI0002719 , MI0004735 , MI0002717 , MI0002723 , MI0002725 , MI0005283 , MI0005282 , MI0002720 , MI0002722 , MI0002721 , MI0002718 , MI0001270 , MI0016974 , MI0007610
MIMAT0000133 , MIMAT0000099 , MIMAT0000823 , MIMAT0002427 , MIMAT0003520 , MIMAT0002425 , MIMAT0002431 , MIMAT0002433 , MIMAT0004098 , MIMAT0002428 , MIMAT0002430 , MIMAT0002429 , MIMAT0002426 , MIMAT0001185
MIR-101-101C
35823
MIR-101-101C
4
MI0000266 , MI0000685 , MI0000841 , MI0002791 , MI0005007 , MI0002788 , MI0005273 , MI0002792 , MI0002790
MIMAT0000782 , MIMAT0000648 , MIMAT0000253 , MIMAT0002489 , MIMAT0003786 , MIMAT0002486 , MIMAT0004089 , MIMAT0002490 , MIMAT0002488
MIR-10A
35824
MIR-10A
5
MI0000442 , MI0000256 , MI0000891 , MI0005063 , MI0005287 , MI0002413 , MI0001277 , MI0001280
MIMAT0000827 , MIMAT0000246 , MIMAT0000421 , MIMAT0003849 , MIMAT0004101 , MIMAT0002119 , MIMAT0001190
MIR-122
35826
MIR-122
6
MI0000897 , MI0000446 , MI0000470 , MI0000152 , MI0000725 , MI0000896 , MI0002510 , MI0002575 , MI0004753 , MI0005457 , MI0002509 , MI0002574 , MI0002514 , MI0002579 , MI0002517 , MI0002581 , MI0005291 , MI0005292 , MI0002511 , MI0002513 , MI0002578 , MI0002512 , MI0002577 , MI
MIMAT0000830 , MIMAT0000136 , MIMAT0000423 , MIMAT0002221 , MIMAT0003539 , MIMAT0002220 , MIMAT0002225 , MIMAT0002228 , MIMAT0004103 , MIMAT0002222 , MIMAT0002224 , MIMAT0002223 , MIMAT0002226 , MIMAT0002120 , MIMAT0001105
MIR-125B
35830
MIR-125B
7
MI0000471 , MI0000153 , MI0000898 , MI0004754 , MI0001244
MIMAT0000137 , MIMAT0000444 , MIMAT0000831 , MIMAT0004328 , MIMAT0003723
MIR-126-5P
35832
MIR-126-5P
8
MI0000473 , MI0000585 , MI0000637 , MI0000902
MIMAT0000601 , MIMAT0000544 , MIMAT0004605 , MIMAT0017120
MIR-129-3P
35835
MIR-129-3P
9
MI0000252 , MI0000473 , MI0000222 , MI0000585 , MI0000637 , MI0000902 , MI0005294
MIMAT0000600 , MIMAT0000209 , MIMAT0000242 , MIMAT0004105
MIR-129-5P
35836
MIR-129-5P
10
MI0000448 , MI0000156 , MI0000903 , MI0002525 , MI0002524 , MI0002526 , MI0002527
MIMAT0000836 , MIMAT0000141 , MIMAT0000425 , MIMAT0002236 , MIMAT0002235 , MIMAT0002237 , MIMAT0002238
MIR-130A
35837
MIR-130A
11
MI0000904 , MI0000748 , MI0000408
MIMAT0000837 , MIMAT0000387 , MIMAT0000691
MIR-130B
35838
MIR-130B
12
MI0000474 , MI0000160 , MI0000907 , MI0002589 , MI0002591 , MI0002592 , MI0002590
MIMAT0000840 , MIMAT0000447 , MIMAT0000146 , MIMAT0002288 , MIMAT0002290 , MIMAT0002291 , MIMAT0002289
MIR-134
35841
MIR-134
13
MI0000475 , MI0000162 , MI0000909 , MI0002594 , MI0001523 , MI0002596 , MI0002593 , MI0002595 , MI0002452
MIMAT0000148 , MIMAT0000448 , MIMAT0000842 , MIMAT0002293 , MIMAT0001417 , MIMAT0002295 , MIMAT0002292 , MIMAT0002294 , MIMAT0002158
MIR-136
35843
MIR-136
14
MI0000454 , MI0000163 , MI0000910 , MI0005299 , MI0001221
MIMAT0000843 , MIMAT0000429 , MIMAT0000149 , MIMAT0004110 , MIMAT0001153
MIR-137
35844
MIR-137
15
MI0000456 , MI0000611 , MI0000165 , MI0002547 , MI0002437 , MI0001229
MIMAT0000573 , MIMAT0000431 , MIMAT0000151 , MIMAT0002255 , MIMAT0002143 , MIMAT0001159
MIR-140-5P
35847
MIR-140-5P
16
MI0000457 , MI0000166 , MI0000914 , MI0002487 , MI0002485 , MI0002489
MIMAT0000846 , MIMAT0000153 , MIMAT0000432 , MIMAT0002198 , MIMAT0002196 , MIMAT0002200
MIR-141
35849
MIR-141
17
MI0000917 , MI0000460 , MI0000168 , MI0002556 , MI0002557 , MI0002554 , MI0002555
MIMAT0000156 , MIMAT0000436 , MIMAT0000850 , MIMAT0002264 , MIMAT0002265 , MIMAT0002262 , MIMAT0002263
MIR-144
35854
MIR-144
18
MI0000461 , MI0000169 , MI0000918 , MI0004756 , MI0002560 , MI0002558 , MI0002562 , MI0005305 , MI0002559 , MI0002561 , MI0002417
MIMAT0000851 , MIMAT0000157 , MIMAT0000437 , MIMAT0003542 , MIMAT0002268 , MIMAT0002266 , MIMAT0002270 , MIMAT0004116 , MIMAT0002267 , MIMAT0002269 , MIMAT0002123
MIR-145
35855
MIR-145
19
MI0000477 , MI0000170 , MI0000919 , MI0001235
MIMAT0000852 , MIMAT0000158 , MIMAT0000449 , MIMAT0001163
MIR-146A
35856
MIR-146A
20
MI0000253 , MI0000550 , MI0004737 , MI0002418 , MI0001189
MIMAT0000516 , MIMAT0000243 , MIMAT0003522 , MIMAT0002124 , MIMAT0001120
MIR-148A
35857
MIR-148A
Total number of rows: 1557 Table truncated, full table size 127 Kbytes .
Supplementary data files not provided