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Status
Public on Dec 31, 2015
Title
DNA microarray for STD pathogen detection
Technology type
spotted DNA/cDNA
Distribution
non-commercial
Organisms
Neisseria gonorrhoeae ; Chlamydia trachomatis ; Mycoplasmoides genitalium ; Metamycoplasma hominis ; Ureaplasma ; Human alphaherpesvirus 1 ; Human alphaherpesvirus 2 ; Human papillomavirus Manufacturer
in house
Manufacture protocol
The probes were dissolved in 50% dimethyl sulfoxide (DMSO) to a final concentration of 1 µg/µL and coated onto aldehyde group-modified glass slides (CapitalBio Corporation, Beijing, China) using SpotArray 72 (Perkin-Elmer Corporation, CA, USA). Each probe was spotted in triplicate. Coated slides were dried and stored at room temperature in the dark. Each glass slide contained eight individual arrays framed with an 8-sample cover slip containing individual reaction chambers.
Submission date
Jun 03, 2015
Last update date
Dec 31, 2015
Contact name
Boyang Cao
E-mail(s)
boyangcao@nankai.edu.cn Organization name
Nankai Univ
Street address
#23 HongDa Street
City
Tianjin
ZIP/Postal code
300457
Country
China
Samples (17)
GSM1702270 , GSM1702271 , GSM1702272 , GSM1702273 , GSM1702274 , GSM1702275
GSM1702276 ,
GSM1702277 ,
GSM1702278 ,
GSM1702279 ,
GSM1702280 ,
GSM1702281 ,
GSM1702282 ,
GSM1702283 ,
GSM1702284 ,
GSM1702285 ,
GSM1702286 Series (1)
GSE69508 DNA microarray for STD pathogen detection
Data table header descriptions ID POSITION PROBE ID Organism SPOT_ID Target gene GB_ACC GenBank Accession number
SEQUENCE
Data table ID POSITION PROBE ID Organism SPOT_ID Target gene GB_ACC SEQUENCE 1
1-1
Cy3
positional probe
CONTROL
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-Cy-3
2
1-2
Cy3
positional probe
CONTROL
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-Cy-3
3
1-3
Cy3
positional probe
CONTROL
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-Cy-3
4
1-4
OA-2330
HPV type 54
L1
U37488 ACTGTCARGGTTACCTGAGGATTTC
5
1-5
OA-2330
HPV type 54
L1
U37488 ACTGTCARGGTTACCTGAGGATTTC
6
1-6
OA-2330
HPV type 54
L1
U37488 ACTGTCARGGTTACCTGAGGATTTC
7
1-7
OA-2334
HSV type 1
gB
M21629 GCGGCTCTGCTCTCGGAGGTGTTCC
8
1-8
OA-2334
HSV type 1
gB
M21629 GCGGCTCTGCTCTCGGAGGTGTTCC
9
1-9
OA-2334
HSV type 1
gB
M21629 GCGGCTCTGCTCTCGGAGGTGTTCC
10
1-10
OA-2335
HSV type 1
gB
M21629 CGATGGCAACGCGGCCCAACATATC
11
1-11
OA-2335
HSV type 1
gB
M21629 CGATGGCAACGCGGCCCAACATATC
12
1-12
OA-2335
HSV type 1
gB
M21629 CGATGGCAACGCGGCCCAACATATC
13
2-1
OA-2321
HPV type 58
L1
D90400 AAATGCACTACTTTGGATAACTGCA
14
2-2
OA-2321
HPV type 58
L1
D90400 AAATGCACTACTTTGGATAACTGCA
15
2-3
OA-2321
HPV type 58
L1
D90400 AAATGCACTACTTTGGATAACTGCA
16
2-4
50%DMSO
CONTROL
17
2-5
50%DMSO
CONTROL
18
2-6
50%DMSO
CONTROL
19
2-7
OA-2338
HSV type 2
gB
EU018079 GCGCCCCAGCATGTCGTTCACGTGG
20
2-8
OA-2338
HSV type 2
gB
EU018079 GCGCCCCAGCATGTCGTTCACGTGG
120 7 Kbytes .Supplementary file Size Download File type/resource GPL20279_STD_array.gal.gz
733 b
(ftp) (http) GAL
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