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Status
Public on Sep 24, 2005
Title
UC Human 22K v2.2 Operon 70-mer oligo array
Technology type
spotted oligonucleotide
Distribution
custom-commercial
Organism
Homo sapiens
Manufacturer
Genomics and Microarray Laboratory, University of Cincinatti Medical Center
Manufacture protocol
Aminosilane-coated slides (Cell Associates, Cat#CSA-100 C) are used. Printing 1. The Operon 70-mer oligonucleotides were suspended in 3XSSC. The DNA is printed on aminosilane-coated slides (Cell Associates, Cat.# CSA-100 C). UV-Crosslinking and storage 1. Lay slides array-side-up in a Stratalinker and irradiate at 6000 (x100) mjoules (600mJ). 2. Store slides at room temperature under vacuum. Notes: The 70 mer oligonucleotides are spotted at 22ÂșC and 65-85% relative humidity at ~100 mm center-spacing using a high-speed robotic machine (OmniGrid-100; GeneMachines; San Carlos, CA) using Stealth pins (SMP3 pins) from TeleChem (Sunnuvale, CA), mounted on a 48-pin head. Spot volumes are 0.5 nl and spot diameters are 75-85 mm.
Catalog number
Human Oligo Microarray slides (V2-2)
Support
glass
Coating
aminosilane
Description
Human 70-mer oligo library (V2.2): Representing a total of 22,102 annotated genes.
Web link
http://microarray.uc.edu/INDEX.HTM
Submission date
Sep 23, 2005
Last update date
Jan 18, 2013
Contact name
Rajkumar T
E-mail(s)
rajkumart@yahoo.com
Phone
+91-44-22350340
Organization name
Cancer Institute (WIA)
Department
Molecular Oncology
Lab
Molecular Oncology
Street address
Adyar
City
Chennai
State/province
Tamilnadu
ZIP/Postal code
600020
Country
India
Samples (4)
GSM76017 , GSM76018 , GSM76019 , GSM76020
Series (1)
GSE3362
Genes involved in doxorubicin drug resistance in 143b osteosarcoma cell line
Data table header descriptions
ID
ROW
This gives the row co-ordinates of the array spots.
COLUMN
This gives the column co-rdinates of the array spots.
Oligo_ID
This is the Operon ID number for the oligo sequences spotted on the array.
GB_ACC
This is the GeneBank Accession number for the gene.
SPOT_ID
UNIGENE
This is the Unigene identification number for the gene.
Gene_Symbol
This is the abbreviated gene label.
Description
This gives the gene name and / or description
Data table
ID
ROW
COLUMN
Oligo_ID
GB_ACC
SPOT_ID
UNIGENE
Gene_Symbol
Description
1
1
1
H200000100
NM_000911
372
OPRD1
Opioid receptor, delta 1
2
1
2
H200000106
NM_001124
394
ADM
Adrenomedullin
3
1
3
H200000124
NM_004024
460
ATF3
Activating transcription factor 3
4
1
4
H200000130
NM_002202
505
ISL1
ISL1 transcription factor, LIM/homeodomain, (islet-1)
5
1
5
H200000148
NM_000384
585
APOB
Apolipoprotein B (including Ag(x) antigen)
6
1
6
H200000154
NM_002306
621
LGALS3
Lectin, galactoside-binding, soluble, 3 (galectin 3)
7
1
7
H200000172
NM_000100
695
CSTB
Cystatin B (stefin B)
8
1
8
H200000178
NM_003250
724
THRA
Thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homo
9
1
9
H200000480
NM_000277
1870
PAH
Phenylalanine hydroxylase
10
1
10
H200000486
NM_000892
1901
KLKB1
Kallikrein B, plasma (Fletcher factor) 1
11
1
11
H200000504
NM_000180
1974
GUCY2D
Guanylate cyclase 2D, membrane (retina-specific)
12
1
12
H200000510
NM_003140
1992
SRY
Sex determining region Y
13
1
13
H200000528
NM_000373
2057
UMPS
Uridine monophosphate synthetase (orotate phosphoribosyl transferase and orotidine-5'-
14
1
14
H200000534
NM_000956
2090
PTGER2
Prostaglandin E receptor 2 (subtype EP2), 53kD
15
1
15
H200000552
NM_000760
2175
CSF3R
Colony stimulating factor 3 receptor (granulocyte)
16
1
16
H200000558
NM_006685
2207
PROL3
Proline rich 3
17
1
17
H200000860
NM_014403
3972
SIAT7D
Sialyltransferase 7D ((alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetyl gala
18
1
18
H200000866
AL117595
4055
Homo sapiens mRNA; cDNA DKFZp564C2063 (from clone DKFZp564C2063)
19
1
19
H200000884
AK024956
4194
Homo sapiens cDNA: FLJ21303 fis, clone COL02107
20
1
20
H200000890
AL359592
4221
DKFZp761H039
Hypothetical protein DKFZp761H039
Total number of rows: 32256 Table truncated, full table size 2142 Kbytes .
Supplementary data files not provided