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Platform GPL2869 Query DataSets for GPL2869
Status Public on Sep 24, 2005
Title UC Human 22K v2.2 Operon 70-mer oligo array
Technology type spotted oligonucleotide
Distribution custom-commercial
Organism Homo sapiens
Manufacturer Genomics and Microarray Laboratory, University of Cincinatti Medical Center
Manufacture protocol Aminosilane-coated slides (Cell Associates, Cat#CSA-100 C) are used.
Printing
1. The Operon 70-mer oligonucleotides were suspended in 3XSSC. The DNA is printed on aminosilane-coated slides (Cell Associates, Cat.# CSA-100 C).

UV-Crosslinking and storage
1. Lay slides array-side-up in a Stratalinker and irradiate at 6000 (x100) mjoules (600mJ).
2. Store slides at room temperature under vacuum.
Notes: The 70 mer oligonucleotides are spotted at 22ÂșC and 65-85% relative humidity at ~100 mm center-spacing using a high-speed robotic machine (OmniGrid-100; GeneMachines; San Carlos, CA) using Stealth pins (SMP3 pins) from TeleChem (Sunnuvale, CA), mounted on a 48-pin head. Spot volumes are 0.5 nl and spot diameters are 75-85 mm.
Catalog number Human Oligo Microarray slides (V2-2)
Support glass
Coating aminosilane
 
Description Human 70-mer oligo library (V2.2): Representing a total of 22,102 annotated genes.
 
Web link http://microarray.uc.edu/INDEX.HTM
Submission date Sep 23, 2005
Last update date Jan 18, 2013
Contact name Rajkumar T
E-mail(s) rajkumart@yahoo.com
Phone +91-44-22350340
Organization name Cancer Institute (WIA)
Department Molecular Oncology
Lab Molecular Oncology
Street address Adyar
City Chennai
State/province Tamilnadu
ZIP/Postal code 600020
Country India
 
Samples (4) GSM76017, GSM76018, GSM76019, GSM76020
Series (1)
GSE3362 Genes involved in doxorubicin drug resistance in 143b osteosarcoma cell line

Data table header descriptions
ID
ROW This gives the row co-ordinates of the array spots.
COLUMN This gives the column co-rdinates of the array spots.
Oligo_ID This is the Operon ID number for the oligo sequences spotted on the array.
GB_ACC This is the GeneBank Accession number for the gene.
SPOT_ID
UNIGENE This is the Unigene identification number for the gene.
Gene_Symbol This is the abbreviated gene label.
Description This gives the gene name and / or description

Data table
ID ROW COLUMN Oligo_ID GB_ACC SPOT_ID UNIGENE Gene_Symbol Description
1 1 1 H200000100 NM_000911 372 OPRD1 Opioid receptor, delta 1
2 1 2 H200000106 NM_001124 394 ADM Adrenomedullin
3 1 3 H200000124 NM_004024 460 ATF3 Activating transcription factor 3
4 1 4 H200000130 NM_002202 505 ISL1 ISL1 transcription factor, LIM/homeodomain, (islet-1)
5 1 5 H200000148 NM_000384 585 APOB Apolipoprotein B (including Ag(x) antigen)
6 1 6 H200000154 NM_002306 621 LGALS3 Lectin, galactoside-binding, soluble, 3 (galectin 3)
7 1 7 H200000172 NM_000100 695 CSTB Cystatin B (stefin B)
8 1 8 H200000178 NM_003250 724 THRA Thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homo
9 1 9 H200000480 NM_000277 1870 PAH Phenylalanine hydroxylase
10 1 10 H200000486 NM_000892 1901 KLKB1 Kallikrein B, plasma (Fletcher factor) 1
11 1 11 H200000504 NM_000180 1974 GUCY2D Guanylate cyclase 2D, membrane (retina-specific)
12 1 12 H200000510 NM_003140 1992 SRY Sex determining region Y
13 1 13 H200000528 NM_000373 2057 UMPS Uridine monophosphate synthetase (orotate phosphoribosyl transferase and orotidine-5'-
14 1 14 H200000534 NM_000956 2090 PTGER2 Prostaglandin E receptor 2 (subtype EP2), 53kD
15 1 15 H200000552 NM_000760 2175 CSF3R Colony stimulating factor 3 receptor (granulocyte)
16 1 16 H200000558 NM_006685 2207 PROL3 Proline rich 3
17 1 17 H200000860 NM_014403 3972 SIAT7D Sialyltransferase 7D ((alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetyl gala
18 1 18 H200000866 AL117595 4055 Homo sapiens mRNA; cDNA DKFZp564C2063 (from clone DKFZp564C2063)
19 1 19 H200000884 AK024956 4194 Homo sapiens cDNA: FLJ21303 fis, clone COL02107
20 1 20 H200000890 AL359592 4221 DKFZp761H039 Hypothetical protein DKFZp761H039

Total number of rows: 32256

Table truncated, full table size 2142 Kbytes.




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