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Status
Public on Jul 16, 2003
Title
DGC v1.0
Technology type
spotted DNA/cDNA
Distribution
non-commercial
Organism
Drosophila melanogaster
Description
5928 cDNAs from the Drosophila Gene Collection version 1.0 were amplified by PCR with universal primers and PCR products were confirmed by gel electrophoresis. Added to these was a set of 177 separately amplified controls, each of which was replicated from one to 16 times on the array. Clones for which the PCR product was absent, faint, the incorrect size, or showed multiple bands failed PCR quality control. The PCR products were purified with Multiscreen filter plates (Millipore), dried down, resuspended in 30?L 3X SSC, and mechanically spotted onto polylysine-coated glass slides. The arrays were allowed to air dry following printing and stored in a dessicator. Prior to blocking, arrays were rehydrated by placing them in a humid chamber for 5-7 minutes and then snap dried by placing the slide (array up) on a 80 °C heating block for 3-5 seconds. DNA was then crosslinked to the slide with 60 mJ of UV radiation. Slides were blocked by immersion in a blocking solution (6 g succinic anhydride, 335 mL 1-methyl-2-polypyrolidinone, 15 mL 1 M sodium borate) and shaken at room temperature for 20 minutes. They were then washed in boiling water for 2 minutes, rinsed in 95% ethanol, and spun dry.
Contributor(s)
Meiklejohn CD , Parsch J , Ranz JM , Hartl DL Citation(s)
12907700 Submission date
Jul 12, 2003
Last update date
Jan 18, 2013
Contact name
Colin Meiklejohn
E-mail(s)
meiklej@fas.harvard.edu Phone
617-496-5540
Fax
617-496-5854
URL
http://www.oeb.harvard.edu/hartl/lab/ Organization name
Harvard University
Department
Organismic and Evolutionary Biology
Lab
Hartl Lab
Street address
16 Divinity Ave
City
Cambridge
State/province
MA
ZIP/Postal code
02138
Country
USA
Samples (56)
GSM7863 , GSM7864 , GSM7865 , GSM7866 , GSM7867 , GSM7868
GSM7869 ,
GSM7870 ,
GSM7871 ,
GSM7872 ,
GSM7873 ,
GSM7874 ,
GSM7875 ,
GSM7876 ,
GSM7877 ,
GSM7878 ,
GSM7879 ,
GSM7880 ,
GSM7881 ,
GSM7882 ,
GSM7883 ,
GSM7884 ,
GSM7885 ,
GSM14975 ,
GSM14976 ,
GSM14977 ,
GSM14978 ,
GSM14979 ,
GSM14980 ,
GSM14981 ,
GSM14982 ,
GSM14983 ,
GSM14984 ,
GSM14985 ,
GSM14986 ,
GSM14987 ,
GSM14988 ,
GSM14989 ,
GSM14990 ,
GSM14991 ,
GSM14992 ,
GSM14993 ,
GSM14994 ,
GSM14995 ,
GSM14996 ,
GSM14997 ,
GSM14998 ,
GSM14999 ,
GSM15000 ,
GSM15001 ,
GSM15002 ,
GSM15003 ,
GSM15004 ,
GSM15005 ,
GSM15006 ,
GSM15007 Series (3)
Data table header descriptions ID BLOCK array block number
ROW array row number
COLUMN array column number
Clone DGC clone identifier
CLONE_ID Flybase identifier
ORF celera predicted gene
GENE_NAME gene name
GB_LIST GB_ACC1 GenBank cDNA sequence accession number
GB_ACC_5'_EST 5' EST sequence GenBank accession number
GB_ACC_3'_EST 3' EST sequence GenBank accession number
PCR quality control of PCR amplification (1 = pass, 0 = fail)
POLYMERASE polymerase used to amplify clone (0 = Taq [Amersham], 1 = Herculase [Stratagene])"
SPOT_ID
Data table ID BLOCK ROW COLUMN Clone CLONE_ID ORF GENE_NAME GB_LIST GB_ACC1 GB_ACC_5'_EST GB_ACC_3'_EST PCR POLYMERASE SPOT_ID 1.1.1
1
1
1
GM13032
FBgn0035896 CG6983
AA801798 , AI513219 AA801798 AI513219 1
0
1.1.10
1
1
10
HL08052
FBgn0002641 CG1692
mal
AA699259 , AI518938 AA699259 AI518938 0
0
1.1.11
1
1
11
HL08191
FBgn0038925 CG6022
AA736226 , AI518948 AA736226 AI518948 1
0
1.1.12
1
1
12
LD21131
FBgn0010226 CG8938
GstS1
AA735338 , AI455098 AA735338 AI455098 1
0
1.1.13
1
1
13
LD21679
FBgn0015286 CG2849
Rala
AA735651 , AI455190 AA735651 AI455190 0
0
1.1.14
1
1
14
LD21713
FBgn0026576 AA735667 , AI455197 AA735667 AI455197 1
0
1.1.15
1
1
15
LD21828
FBgn0031907 CG5171
AA816945 , AI455217 AA816945 AI455217 1
0
1.1.16
1
1
16
LD21907
FBgn0038252 CG3509
AA816969 , AI455221 AA816969 AI455221 1
0
1.1.17
1
1
17
LD22010
FBgn0035524 AA817025 , AI455229 AA817025 AI455229 1
0
1.1.18
1
1
18
LD22559
FBgn0038552 CG18012
AA817319 , AI455280 AA817319 AI455280 1
0
1.1.19
1
1
19
LD23788
FBgn0036144 CG6207
AA820264 , AI455373 AA820264 AI455373 1
0
1.1.2
1
1
2
GM13517
FBgn0036691 CG7842
AA803567 , AI518969 AA803567 AI518969 1
0
1.1.20
1
1
20
LD23846
FBgn0038984 CG5315
AA820298 , AI455386 AA820298 AI455386 1
0
1.1.21
1
1
21
LD23906
FBgn0034182 CG9640
AA820338 , AI455398 AA820338 AI455398 1
0
1.1.3
1
1
3
GM13767
FBgn0031229 CG3436
AA803646 , AI518976 AA803646 AI518976 1
0
1.1.4
1
1
4
GM13912
FBgn0034050 CG8297
AA803675 , AI518981 AA803675 AI518981 1
0
1.1.5
1
1
5
GM13959
FBgn0025366 CG5277
Ip259
AA803684 , AW941376 AA803684 AW941376 1
0
1.1.6
1
1
6
GM14202
FBgn0038183 CG9286
AA803757 , AI518991 AA803757 AI518991 1
0
1.1.7
1
1
7
GM14788
FBgn0040309 CG1633
Jafrac1
AA821176 , AI519021 AA821176 AI519021 1
0
1.1.8
1
1
8
GM14877
FBgn0038976 CG7048
AA821208 , AI519025 AA821208 AI519025 1
0
6101 502 Kbytes .Supplementary data files not provided
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