GEO help: Mouse over screen elements for information.
Status
Public on Apr 30, 2007
Title
GFNChile_Peach_0.9K_v1.0
Technology type
spotted DNA/cDNA
Distribution
non-commercial
Organism
Prunus persica Manufacturer
Chilean Functional Genomics in Prunus persica Consortium: Universidad de Chile, Instituto de Investigaciones Agropecuarias, Asociacion de Exportadores, Fundacion para el Desarrollo Fruticola and Fundacion Chile. Supported by FDI-CORFO (FDI G02 P1001)
Manufacture protocol
The source of the spotted cDNA was a cv. Loring ripe peach fruit (Prunus persica L. Batsch) cDNA library. The library was constructed in the pBluescript II SK(-) vector and acquired in Clemson University Genomics Institute. A total of 847 cDNAs were selected from this library based on the functional properties assigned to their encoded proteins using an in-house gene-ranking method. This analysis involved sequence homology search using tBLASTx of 3,843 non-redundant sequenced clones from the cDNA library against the Arabidopsis CDS database to generate a list of orthologs (e-value <1E-10, identity > 70% over a minimum of 50 aa) which were further analyzed by manual searches in the annotated database, gene ontology terms, and information obtained by literature. Sequence management was performed with database tools developed in our laboratory. Domain-based analyses used SMART and Interpro. Signal peptide prediction and subcellular localization used SignalP and TargetP v1.01 software. Selected EST clones were picked from freezer stocks and grown overnight in 96 well plates in 200 µl LB plus 50 mµ/mL of ampicillin. Overnight cultures were used directly as templates in PCR reactions containing 10X buffer, 0.25 mM each of dATP, dCTP, dGTP, and dTTP, 2.5 units of Taq DNA polymerase and 0.2 µM of T3 and T7 forward/reverse primers. Aliquots (5 µl) of each reaction were loaded onto a 1.0% (w/v) agarose gel to determine product quality. 20 µL of PCR products were arrayed in 96-well plates and mixed with an equal volume of 50% of dimethyl sulfoxide. PCR products (70 ng) were single spotted onto nylon membranes (10 x 7 cm) Immobilon NY+ (Millipore) using an 8 pins print-head (Arraylt model SSP015) and the arraying robot Versarray Chip Writer Compact (BioRad). The membranes were treated and the cDNA was fixed to the membrane by UV-crosslinking using a Ultraviolet Crosslinker CL-1000 (UVP). In addition to the selected ESTs, the following controls were spotted onto membranes: (1) a fragment of the vector pBluescript II obtained by amplification with the T3 and T7 universal primers; (2) several aliquots of DMSO 50%; (3) PpExpansin (GeneBank: AB029083), a gene expressed in peach and (4) cDNA from a B. subtilis gene dap (obtained from ATCC; number 87486) used as spike mRNA to normalize between membranes.
Support
nylon
Coating
other
Web link
http://www.genomavegetal.cl/dr/ Contributor(s)
González-Agüero M , Pavez L , Ibáñez F , Pacheco I , Campos-Vargas R , Meisel L , Orellana A , Retamales J , Silva H , González M , Cambiazo V Submission date
Feb 23, 2007
Last update date
Apr 30, 2007
Contact name
Mauricio Gonzalez
E-mail(s)
maugonzalez@inia.cl Organization name
Instituto de Investigaciones Agropecuarias
Lab
Postcosecha
Street address
Santa Rosa 11610
City
Santiago
State/province
Region Metropolitana
ZIP/Postal code
439-3
Country
Chile
Samples (36)
GSM171976 , GSM171977 , GSM171978 , GSM171979 , GSM171980 , GSM171981
GSM171982 ,
GSM171983 ,
GSM171987 ,
GSM171988 ,
GSM171989 ,
GSM172013 ,
GSM172014 ,
GSM172035 ,
GSM172036 ,
GSM172037 ,
GSM172038 ,
GSM172039 ,
GSM172109 ,
GSM172110 ,
GSM172111 ,
GSM172112 ,
GSM172113 ,
GSM172114 ,
GSM172115 ,
GSM172116 ,
GSM172117 ,
GSM172118 ,
GSM172119 ,
GSM172120 ,
GSM172121 ,
GSM172122 ,
GSM172124 ,
GSM172126 ,
GSM172127 ,
GSM172128 Series (1)
GSE7145 Transcriptional changes associated with woolliness during fruit ripening in Prunus persica
Data table header descriptions ID GB_ACC GenBank accession of Peach developing fruit mesocarp Prunus persica cDNA clone
SEQUENCE Nucleotide sequence of Peach developing fruit mesocarp Prunus persica cDNA clone
CLONE_ID Clone identifier of Clemson peach cDNA library of developing fruit mesocarp
Data table ID GB_ACC SEQUENCE CLONE_ID 101_G01
BU039619 TTGATCGTTGATTCAGGTGGTTTATGATCGTAAACGTGGGGCTTACATTTCTATTTGGAGGGATACTTGGATGGATTTTGGTAAAGATAGTCAGACCAAAACCTTATCAGGAAGGCCTGGTAATGGCTGCATGTTCATCAGGTAAATGCTCAATTTTTTTATATAGTTTTTCATCTCTTTCTTATAGCAGCAAATTTGAAGTGTGCTCATCAAGTAATGGCAAATCTTGTTGAATAGCAAACTTGGGAAACCTTCCCCTTATACTTATCCCTGCAATCTGTGAAGAGGATGGGAACCCATTTGGCGATCATATTGTTTGTAAAAGGGTTGGACTCTCATATGTGTCTTTTTCCATGGCGCTTGGGGGTTTCTTCATCTGGACTTACAGTTACCAGCTGATACGAACTTCAGCGATGAAATGGAAAGCTCTTCAAGCAATTGAGGCAGCTGAGGAGGCCTCAAAGAATCCCAACAGTGATTTAGACGCTGAGGGGGAAACTCCCCTTCTCAAGGGAGAAGATGAAGAGCAGGGTTCTATAGTAGTTTCATCAAATGGTTCTAGAAACCAAGAAGCTGTGAGTACCCTCATATACCAATCTACATAAAAAGTCTGATGAAAACTAGAACTAGTCTGGC
PP_LEa0003F23f 102_D02
BU040135 TTTTTTTTTGAACACACCAAAATGAGGAACTATATAGAAAAAGGGAAGTTTATTTACACGCATTTACCCTATATAACCGATTATTTTCCAGAGCCCCGAATAAATATGCAGGAAATTATTCCCCACAATTGTGACCATCTACACCTGAGGCTAATGAATATCATAGCATACAGCATCATCGGTAGACAGCACCCTACCAAAGCTTCCAGACCCACCCGCCCATTGACGCCTAAGAGGAATTGGGCGGCGGAATATATTATCACACAATGGAGCAGATGCACCGAAAGATCTGCAATGGCAAGATTGCATTTGAAGTCCATCCACTTGCACTGCCTTCCAATATGAAAGAAACCCTATTCGAATCAATGAGGAAAGAACTAGCATTCTCATCTAGCTTGAGAGTTGTCGTCAGCATAGATGGATGCGCAAGCATCTGCAGCACCAGCAGCAAGCAGCACTTCATAGGGATGGAAGGCTAAGCAACTGACAGGACCGATCTTTTGGGCCATGAAAGAAGGGTAGTATCTTATGGTACCTAATTGTTCACCCTCCAAACTAAAGACTTTTATGAGTTGTTTGGCTGATCCACTGGCAATGATGGGGGCATGTCTATGAACAGCTAAAGCTGTCAGTGAACCCC
PP_LEa0004P18f 102_D07
BU040228 CATAGGTGTCCTCCGATACTTCGCCTCCAAGCTCATGCGCTCCAACCAAGTCCCCGATGCAAAAATAATCAAAGAAGGGCAATTGATTATTAGGGCTCGGAATTTACGTGCTGCTGCTAATTTCATACCTCCCAAGTCGTTTCGCGCTCGCCGGTTTTATTTCAGCAATGAGGAAAATGGGTTGTTAATTGTTCCCAAGGGTCAGGTACAGAATGCACAAGCGCAAATGTTCTCTGATCCCAACATGGCTATGGACATGATGAAGAAAAATCTGTCTATGATCATACCCCAGACTCTTACTTTTGCATGGGTCAACTTTTTCTTTTCTGGATTTGTAGCAGCCAAAATACCCTTTCCTCTTACTCAAAGGTTCAGGTCAATGCTGCAGAATGGGATTGACTTAAGCACTGTTGATGTTAGCTATGTCAGTAGCCGCTCATGGTACTTTCTTAATCTGTTTGGATTAAGAGGTTTGTTTAGTCTCATTCTGGGAGAAGAAAATGCAATGGATGATACTCAGCGTATGATGCAAATGAGTGNGGTTTGGTTTTGATCCATCAAAGAGCTTGGGCGCTGAGAAGGACAGTCTTGACATAGTCCAGCATGATTGGGCCTTACCAAAATTTGAGCACCGTGCT
PP_LEa0005E15f 102_E01
BU040304 GGCCGCTAATACACTAGTGGATCCCCCGGCTGCTTATCAATTTACAGCAAAACAAACAAAGCTTCGAAACATGTCACCGNGAAAATGAGCAGCAGACGCCGGCCGGCTTGAACTACGACGATACCAAGTTGACCTTGGGGTTGCCTGGCTCGGGGTCTAAGCGTGGATTTTCAGAGACCATTGATCTTAGTCTTGGGAGCTCAAGCTCATCGTCGTCAAGATCATGGTGTTTATGTTGTGATCAAGATTGTGCTGATGGTGATGGGGCCAAAACTCATAAGCCTCCACCCCCAAAGTCACAAGTGGTGGGGTGGCCGCCAGTGAGAGTGGCAAGGAA
PP_LEa0005I24f 102_F12
BU040689 CATATAGTCATCAACGACTGCCAAAATGTGCAAATGCAAGGTGTCAGGGTTTCTGCTTCCGGTAATAGCCCTAACACCGATGGCATTCATGTCCAAATGTCATCTGGTGTCACAATCCTCAACTCCAAGATTGCAACCGGTGACGATTGTGTCTCAATTGGCCCCGGAACCTCAAATTTGTGGATAGAAGGCGTTGCTTGTGGACCTGGCCATGGAATTAGCATTGGAAGTCTAGGCAAGGAGCAAGAAGAGGCCGGTGTACAAAATGTAACAGTTAAAACGGTTACCTTTAGTGGTACTCAGAATGGTCTAAGAATCAAGTCATGGGGGAGGCCAAGCACTGGGTTTGCTAGAAATATTCTTTTCCAACATGCTACAATGGTCAATGTCGAAAATCCTATTGTCATAGATCAACATTATTGCCCCGACAACAAAGGGTGCCCTGGTCAGGTTTCCGGAGTTCAAATTAGCGATGTGACATACGAAGACATACACGGTACATCAGCAACAGAAGTTGCAGTAAAATTTGATTGCAGTCCCAAGCACCCTTGCAGCGAGATCAAATTGGAGGATGTGAAGCTTACTTACAAGAACCAAGCAGCTGAGTCTTCATGTAGCCATGCAAATGGAACAACTGATGGTGTGGTTCAGCCTACAAGTTGTTTGTAGAGTGGAAAAATATGAACTGCTTGTACAAGTATTG
PP_LEa0006P14f 102_G08
BU040719 AGCAAGACTCAATGGCTTTGCCGAATCAGCAGACCGTGGATTACCCGAGCTTCAAGCTCGTAATCGTTGGCGATGGTGGAACAGGAAAAACTACCTTTGTGAAGAGACATCTTACTGGTGAATTTGAGAAGAAATACGAACCAACTATTGGCGTGGAGGTCCATCCATTGGACTTTTTCACCAACTGTGGAAAAATTAGATTCTATTGCTGGGACACAGCCGGGCAAGAGAAATTTGGTGGTCTTAGAGATGGATACTACATCCATGGACAATGTGCAATTATCATGTTTGATGTTACTGCTCGATTGACATACAAGAATGTTCCGACTTGGCATCGGGATCTTTGCAGGGTCTGTGAGAACATTCCAATTGTTCTTTGTGGAAACAAGGTTGATGTGAAGAACAGGCAGGTCAAGGCAAAGCAGGTTACTTTCCACAGGAAGAAGAATCTGCAGTACTATGAGATATCAGCAAAGAGCAACTATAACTTTGAGAAGCCTTTCCTATACCTTGCCAGAAAGCTTGCTGGGGATCCTAATTTGCATTTTGTTGAGTCACCTGCCTTGGCTCCTCCAGAAGTACACATTGATCTGGCTGCACAACAACAGCACGAAGCCGAGCTCGCTGCAGCTGCCAGCCAGCCCCTTCCTGATGATGACGATGA
PP_LEa0007B05f 102_H04
BU040795 AACAAGTTCCCANAGAGGCAGCCTATTGGGACATCAGCTCAGACAGACAAGGACTACAAGGAGCCTCCCCCAGCTCCGTTGTTTGAGCCTGGTGAGCTCACTTCATGGTCTTTTTACAGAGCTGGAATTGCTGAGTTCATTGCCACCTTCTTGTTCCTCTACATCACCATCTTGACTGTGATGGGTGTGAACAGGGCTCCTAGTAAGTGTGCCTCTGTTGGCATCCAAGGAATTGCTTGGGCTTTTGGTGGCACCATCTTTGCCCTTGTCTACTCCACTGCTGGTATCTCAGGTGGACACATCAACCCAGCTGTGACCTTTGGTCTCTTCCTGGCAAGGAAACTCTCCCTTACAAGAGCTGTTTTCTACATAGTCATGCAGACCCTTGGTGCTATTGCTGGTGCTGGTGTTGTGAAGGGCTTCGAAAAGAAACAATATGAGCTTTTGGGAGGTGGAGCCAATGTTGTTAACCATGGGTACANCCAAGGTGGTGGCCTTGGGGCTGAGATTGTTGGCACTTTTGTCCTTGTTTACACTGTCTTCTCTGCCACTGATGCCAAGAGGAATGCCAGAGACTCTCATGTCCCTATTTTGGCTCCTCTTCCCATTGGGTTTGCAG
PP_LEa0007F01f 103_B04
BU041025 AGGATTGGAGGTCAAGCCATGGATTAAGACGAGTCTTGCTCCGGGTTCTGGGGTTGTAACCAAATACTTACAAAAGAGTGGGTTGCAGCAGTATTTGAATCAGTTAGGGTTTCATATTGTTGGGTATGGATGCACCACATGCATTGGGAATTCAGGAGATATTGATGACGCTGTGGCATCAGCTATTACAGAAAATGATATTGTAGCTGCGGCTGTGTTGTCTGGAAATAGGAATTTTGAGGGTCGTGTGCATCCGTTAACAAGGGCCAATTATCTTGCATCTCCTCCCCTTGTTGTTGCCTATGCTCTTGCTGGAACGGTAGATATTGATTTCGAAACAGAACCAATTGGGTTGGGAAAAGATGGTAAGAAGATATTTTTCAAGGACATTTGGCCATCTAATGAAGAAGTAGCAGAGGTTGTGCAATCAAATGTGCTTCCTCATATGTTCATGGCTACATACGAAGCAATCACCAAAGGAAATCCAATGTGGAATCAGTTATCTGTACCAGATGGCACTCTTTATGCCTGGGACCCAAAATCAACTTATATACATGAGCCACCTTATTTCAAAGATATGACCATGTCTCCTCCGGGCGCTCATGGAGTGAAGAATGCTTACTGCTTGCTC
PP_LEa0008A19f 103_B10
BU041100 CGAAGACGTCTCAAAGTCGAACCACACTGCACTGTACGAAGCATGACCGCCCAAACTCAAGAAGAGCAGCTCGCTGCCCAACTCGAACAGCAAAAAATTCATGATGATGAGCCTGTAGTCGAGGACGAAGATGATGAAGATGACGATGATGATGAAGATGATGAAAAGGATGATGATGATGTGGAAGGACAAGGAGATGCAAGTGGTAGGTCTAAGCAGAGCAGAAGTGAAAAGAAGAGTCGCAAGGCAATGTTGAAACTTGGAATGAAGCCAATACCAGGTGTCAGCCGTGTGACTGTCAAGAAGAGCAAGAATATTCTGTTTGTTATCTCAAAGCCAGATGTTTTTAAGAGCCCTGCATCTGATACGTATGTCATTTTTGGGGAAGCAAAGATTGAAGATTTAAGCTCACAACTTCAAACTCAGGCTGCGGAGCAGTTCAAGGCTCCTAATCTAAATAATGTGACAGCAAACCCTGAGCCATCAACATTGGCTCAAGATGATGAAGATATAGATGAAACTGGTGTGGAACCCAAGGACATAGAGTTGGTGATGACACAAGCTGGGGTTTCAAGACCAAGAGCTGTTAAAGCACTCAAAGCAGCAGAAGGAGACATTGTTTCTGCCATTATGGAATTAACAAACTGA
PP_LEa0008E23f 103_C07
BU041188 CGATATACCCTACAAAATTGTGGAGGTCAACCCACTTAGTAAGAAAGAGATCAAATGGTCTGATTATAAGAAGGTTCCTATATTGATGGTGGATGGGGAACAGCTGGTTGACTCATCAGCTATAATTGATAAGTTGAATGACAAAATTGTTCCTGAGAGAGTAGCCGCCCCTTCCTCAAATGATGATGAAGAGAAAAAGTGGCGCCAGTGGGTTGATAATCACTTGGTGCATATGTTATCACCAAACATATACCGAAATACATCTGAGGCCCTTGAATCCTTTGACTATATAACAAGCAATGGTAATTTTAGCTACACAGAAAAATTTACAGTGAAGTATGCTGGTGCTGCGGCTATGTACTTTGTGTCAAAGAAACTGAAGAAGAAATATAATATTACGGATGAACGTGCATCCCTGTATGAAGCAGCAGAAACATGGGTTAATGCTCTTAATGGGCGGGACTTTCTTGGGGGCTCCAAACCTAATATGGCCGACCTTGCTGTTTTTGGGGTGCTAAGACCAATTCGTTATTTGAGGTCTGGTAAAGATATGGTGGAGCACACACGCATAGGTGAATGGTACTCAAGAATGGAGCGTGCCGTTGGAGAGTCTGCAAGGATTAAAGCCTAAATTGGATATCCTATGGAGTNTG
PP_LEa0008I23f 103_C12
BU041223 GGATAATATGTCGAAGACAGGTGAGGTCGTATGCGTTACCGGTGGAAGTGGGTGCATTGGATCATGGCTCGTCCGCCTCCTCCTCGACCGTAATTACACCGTCCATGCCACCGTCAAAGATCTCAAGGACGAGGGTGAGACGAAGCATCTAGAAGCCCTAGAAGGAGCAGCGACGCGTCTCCGTCTCTTCCAGATCGACCTCCTCGACTACAACTCCATCTTCGCCGCCATCAATGGCTGCTCCGGCGTCTTCCACCTCGCCTCTCCTTGCATCATCGATCAAGTCCAAGACCCCGAGAAGGAGCTTCTGGACCCGGCGATCAAAGGAGCGCTTAATGTTCTGACGGCAGCGAAGCAAGCTGGGGTCAGGCGAGTGGTGCTGACATCCTCCAACGCCGCCATCATTCCCAGCCCTTGCTGGCCGTCCGATAAGATCAAGGGTGAGGATTGCTGGACCGATATTGACTACTGCAAGCAGAAGGGATTGTGGTATTCATTGTCGAAAACTTTGGCGGAGAAAGCTGCGTGGGAATTTGCCAAGGAGAAGGGATTGGATGTGGTTGTGGTGAATCCTGGCACCGTGTTGGGCGATGTCATTTCGCCCAGGCTCAATTCTAGCATGGTGA
PP_LEa0008K15f 103_F09
BU041499 CTGCTATCGATCTCCGAAGCCAGCAATGGAGAACCTGATCTCATTGGTGAACAAAATCCAAAGAGCTTGTACAGCTCTAGGTGACCATGGCGAAGCTAGCGCATTACCGACGCTCTGGGACTCCCTTCCCGCCATCGCCGTCGTCGGTGGCCAGAGTTCAGGGAAGTCTTCTGTCTTGGAGAGCATTGTTGGGAAGGACTTCTTACCACGTGGTTCTGGAATTGTTACCCGGCGTCCGCTTGTTTTACAACTTCATAAGATTGAAGAAGGCAGCAGAGAATATGCGGAGTTCCTCCATCTTCCTAGGAAGCGGTTCACTGATTTTGCTGCTGTGAGAAAGGAGATTTCAGATGAGACAGATCGAGAAACTGGCCGCAGCAAACAAATTTCTAGTGTTCCAATTCATCTTAGCATATATTCTCCTAATGTTGTCAACTTGACACTTATTGATCTTCCCGGGCTTACAAAAGTGGCTGTTGAGGGTCAATCGGATACTATTGTGCAAGACATTGAAAATATGGTTCGCTCCTACATTGAGAAGCCCAACTGTATAATTCTAGCAATTTCACCTGCTAATCAAGATCTTGCTACATCTGATGCAATTAGAATCTCTCGTGAAGTGGAT
PP_LEa0009I13f 103_G02
BU041547 GCTCTTANAACTAGTGGNATCCCCCGGNGCTGCAGGGNAAGAAGCAAGAGATGGCTCGGACGACGTCGTTTTTGCTCTGCACCCTGGTCCTCTTTGGAACCCTAGCTCTCATCCAGGCAAAGAAGTCAAAGGAGGATCTGAAGGAAGTGACCCACAAAGTTTTCTTTGATGTTGAGATTGCTGGCAAGCCTGCTGGTCGGGTTGTAATCGGTCTCTTTGGGAAGACGGTTCCCAAGACTGCAGAAAATTTTCGAGCCCTTTGCACAGGGGAGAAGGGCATTGGAAAGAGTGGAAAACCTCTCCATTTCAAGGGTAGCAAATTTCATAGGATTATACCCAGTTTCATGATTCAGGGAGGTGATTTCACACTTGGTGATGGAAGAGGTGGAGAATCAATTTACGGAGAGAAGTTTGCTGATGAGAATTTCAAGCTGAAGCATACTGGCCCAGGGCTTTTGTCAATGGCAAATGCTGGTTCCGACACCAATGGTTCACAATTCTTCATCACAACTGTGACAACTAACTGGTTGGATGGCAGACATGTTGTATTTGGAAAGGTGCTGTCTGGAATGGATGTGGTTTACAAGATTGAAGCCGANAGAAATCAGAATGGCACACCCAAGAGCAAAGTTGTGATCGCAGACAGTGGCGAACTTCCTCTCTAGATGTTGCTGTCTTTCTNCACATATTGAGTGATAGT
PP_LEa0009K22f 103_G03
BU041553 CGACCCATTTGATGTGTTCGAACCCGTTGCCCGAAGAGGGCCCCACAAACCCGGGTCGGTGGGGCTAGCCGTGGGTCAGGAGCTGGCGATTTTGGACGAGAAGGGTTTGGTTCAGCCAGAGGGCGTGAACGGCGAGGTGTGCATCAGAGGACCCAATGTGACCAAGGGGTATAAGAACAACCCAGAAGCCAACAAAGCGGCTTTCTTGTTCGGGTGGTTTCATACTGGAGATATCGGCGTTTTGGATTCTGATGGGTATTTGAGCCTCGTGGGTCGGATCAAGGAGCTAATTAACCGCGGAGGGGAGAAGATATCACCCATTGAAGTAGATGCAGTGCTTGTGTCACATCCAGAAATTGAGCAAGGAGTTTCCTTTGGAGTTCCTGATGATAAATATGGTGAAGAGATTAACTGTGCCGTAATCCCAAGAAAAAGCGCAAGCATAGACGAGGAAGAAGTGCTGCGATTTTGCAAGAAGAATCTGGCTTCTTTCANAGTACCCAAGAGGGCGTTCATCACTGACTCTCTTCCTAAAACTGCAACCGGGAAAATCCAACGTTCAATTGTGGCCGATCATTTCCTTGCACAAATCTTCACTGCCAATGTTCCCAAGTTTGGGGCTTAATTTTTCTTATTCCTCAAAAATCCTTGC
PP_LEa0009L09f 103_G07
BU041591 TTTGCTGTGACCGATTGCTTCAGCTACCGTCCGCGCGAAACCAAATGCCGTTTCGCTTTGGGCGATATGTCTGTCCTCGCCAATGAAACCAGATTTACCGTTAATGAGCTGGAGGCATTGTATGAGCTGTTTAAGAAGCTGAGTAGTTCAATTATCGACGACGGATCGATTCATAAGGAAGAGCTTCAATTAGCACTATTCAGAACTCCCTATGGTGAAAATCTTTTCCTGGACAGGGTTTTTGATCTCTTTGATGAAAAGAAAAATGGTGTCATTGAATTTGATGAGTTTGTTCATGCACTCAATGTCTTCCATCCATATGCCCCTATAGATGATAAAATTGATTTTGCATTTAGGCTCTATGATCTGAGACAAACCGGGTTCATTGAGCGAGAAGAAGTTAAGCAAATGGTGATTGCCATAATGATGGAATCTGACTTGAAATTGTCAGATGATCTTCTTGAGTCTATCATTGATAAGACATTTGCCGACGCTGATGCTGACAAAGATGGTAAAATTAGCAAAGAAGACTGGAAGGCTTTTGTGTTTCGACATCCAACCCTTTTGAAAAACATGACTCTTCCTTATTTGAAAGACATCACCGTGGTATTTCCAAGTNTCATTTTTTACACT
PP_LEa0009N08f 104_A08
BU041854 CTCCTAAATGCCAACTGTGACTTAAAAATTGTGATTTTGGACTAGCTCGAGTCACCTCCGAGACTGATTTTATGACTGAGTATGTTGTTACAAGATGGTACCGTGCGCCAGAGCTTCTGTTAAACTCTTCAGATTACACTGCAGCTATTGATGTATGGTCGGTCGGTTGTATTTTCATGGAATTGATGGATAGGAAGCCATTGTTTCCTGGCAGAGATCATGTGCATCAACTTCGTCTGCTTATGGAGCTGATTGGCACCCCATCAGAGACTGAGCTGGGGTTTTTAAATGAAAATGCCAAGAGATATATTCGGCAGCTTCCTCCGCACCGTCGACAGTCATTGACTGAGAAGTTCCCTCACGTCCATCCTTCAGCTATTGATCTTGTTGAAAAGATGTTGACATTTGATCCTACAAAGAGAATTACAGTTGAAGATGCCCTAGCTCACCCCTACTTAACATCTCTCCACGACATCAGTGATGAGCCTGTTTGCATGACTCCCTTCAGCTTCGACTTTGAGCAGCACGCACTGACTGAGGAACAGATGAAAGAGCTGATCTACCGAGAGGCACTTGCATTTAACCCCGAGTATCAGCCACAATGAGAGGAATCATGCAATG
PP_LEa0010L12f 104_C04
BU042097 AATATTCACCTCCTGGAGCAGCTTTTTCTGCAGGTCTAATCCTAGCAATCCCTAGGAGAAAGGCTGGATTACTTGATGATGTCCGCTCTAACTCTTGGCTTGATGCTATGAAATCTTCTTCGCCTCCTCACAGAAAGATAACAAAGGATGTTAACAACGAGCCTGTGGCAAATGAGGCTGATATTGCTTATTCTCTTGGATGGTTAAGTATCCATCAGCACTTACATACTTTGAACAAATCACCAATTATGCCAAGGGCAAGAGAATAGCATTATTTCTGGATTATGATGGGACTCTTTCACCGATTGTAGATAACCCTGACTGTGCCTTTATGTCGGATGCTATGCGCGCAGCTGTAAGAAGGGTGGCAAAACATTTCCCAACAGCAATTATTAGTGGAAGAAGCCATGACAAGGTATATGAGTTTGTACGACTAAAAGAACTCTATTATGCGGGTAGTCATGGGATGGACATAATGGGCCCTGGTAGACAATCTACCGCTGATGACCACATAAATGGTTTTATGACTAGTGACAAACAAGGCGAGGATTGTCAATTTTGTTCCAACCTGCAGCTGAAATTTT
PP_LEa0011H06f 104_C10
BU042172 GTGGATTGGATGGTGGAAATGACATGGATAAGAAACCAGAAGATGGACAATCAGTTGGGAGTCTAAGTGCCGCACCTGAAGTGAAACCTGAGGCAAAATCGGGTGCCGAGGTGGTGAAGAGGACAATCTTGTGGTGGAAGGTTCCATTTGAGGTCTTGAAGTATTGTGTTTTTAGGGTAAGCCCTGTTTGGTCATTTTCTGTGGCCGCAGCAGTGATGGGGTTAGTTTTCTTGGGGCGGCGATTGTATAAGATGAAGAGAAAGAGCCAGACCTTGCAGCTCAAGGTTACTCTGGATGATAAGAAGGTGTCTCAGTTCATGAGTCGCGCTGTGCGTCTCAATGAAGCATTTTCAGTGGTGAGGCGGGTTCCTATAATACGGCCTTCACTTCCAGCATCTGGGGTAAATCCGTGGCCTGTAATGGGCCTGAGATAGAAAATTCTTGTTGTGAAGTACATAGAAAAAGAAAGTACTTTGTCCATGAGCTGTGCTAAAAGTACTAATGTTCTTATGCCACGCTNTGTATCAAAACGATTGTGTCTGTTATGTATTATAACTNTGAAATGTAGAATCTTTTGCATCACTCTGTAGAAAAAAAA
PP_LEa0011K19f 104_D12
BU042385 CTTTGTCAAAAGAAATGGGAATGATGGAAGCTCAGTTGAAGCGGTGGAAGGAAACAGCTCATGAAACACTGTCGTTACGTGACAAAGCCCAATCATTAAAAGCTTCATTAATTACAAAGACACATGAGCAAAAGAGCTTAGCAGATAAATGTGCTGAACAGTTGATAGAGATAAAGTCTCTTAAGGCATTGATTGAGAAGTTGCAGAAAGAAAAACTGGAACTGCAGATCTTCTTAGACTTGTATGCACAAGAAAGCTATGAAAACAGAGACTTGATGGAAATCAAAGAATCAGAACGCAGAGCGTACTCGCAAGCTGAAATGTTCAAAAATGCAATAGATGAACATAGTCTAGAGTTGCGGGTGAAAGCAGCCAATGAAGCTGAAGCTGCTTGTCAGCAAAGGCTNTCTGCTACTGAAGCTGAGATAACTGAATTAAGGGGCAAACTTGATGCATCTGAGAGGGATGTTCTGGAGCTTA
PP_LEa0012G20f 104_F02
BU042550 ATGGGGTGATAAGGGTCTTCAAGAATTATTGAAGATAGTTCAATTCTTGGTGTTGCAGTGGTTTTAGCTGGCCAAGCTCAGGTTGATTTCTCATTGAGGCTGCTCAAGGCAGGGAAGCATGTCCTTCAAGAAAAACCTGCGGCAGCTTCTACAAGCGAATTGGAAACTGCACTGTCAAGTTATAGGTCAATTTTTGCCAATATCCCTGATAAACCAATTTGGGCCGTAGCAGAAAATTATAGATTCGAACCTGCTTTCGTTGAGGGCAAGAAACTAGTGAATGATATTGGAGATGTGATGAGCATCCAAGTTCTTGTTGAAGGATCAATGAACAGTTCAAACCCTTACTTCTCAAGCTCCTGGAGGCGCAATATTACTTGGAGGTTTCATTCTGGACATGGGAGTTCATTTCTTTGCTGGACTGAGGATTCTTGCTGGATGTGAGCTAATTTCTGCCTCCCCTCTATCTCTTTCATGTTTGACCAAACCCTACCTGCCCCAACAACGAATGCTTTTTCCTCTAATTAGAAATGTATGCTCACGGGGTTCCGTTAATTCCCTCTACTTAAATATCCAACCCTTTTTTACGCCTCTTTTAACCTCCTTAATGACTCTTCTCTCTACCCGGAAAACCATATTGGCTACCTCCCCCTCTGGTCT
PP_LEa0013A12f
847 537 Kbytes .Supplementary data files not provided
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