^PLATFORM = GPL5 !Platform_title = Dmel.testis.1.0 !Platform_geo_accession = GPL5 !Platform_status = Public on Oct 08 2000 !Platform_submission_date = Oct 03 2000 !Platform_last_update_date = Oct 28 2005 !Platform_technology = spotted DNA/cDNA !Platform_distribution = non-commercial !Platform_organism = Drosophila melanogaster !Platform_taxid = 7227 !Platform_description = The array was assembled using 1,681 testes ESTs, representing 1,527 Drosophila melanogaster EST sequences and 144 anonymous clones from the bs collection (CLONE_ID). !Platform_description = An aliquot of the same DNA preparation used in the sequencing reactions (GB_ACC), diluted 2/50 in TE, provided material for the PCRs. !Platform_description = Inserts were PCR amplified (1X PCR buffer [Life Technologies], 1.5 mM MgCl2, 0.2 mM dNTPs, 1 µM T7 22-mer primer, 1 µM T3 20-mer primer, 2 µl of plasmid DNA dilution [approximately 10 ng], 0.035 U/µl recombinant Taq DNA polymerase [Life Technologies], in a volume of 100 µl, cycled 94C 2 min, 30X[94 C 0.5 min, 55 C 0.5 min, 2.5 min 72 C], 10 min 72 C in a PTC-225 DNA Engine Tetrad [MJ Research]), and successful amplification was confirmed by agarose gel electrophoresis (PCR-QC: passed = product present, failed = no product). !Platform_description = Samples of approximately 5 nl of 250 µg/ml DNA, in 0.1 N NaOH were printed in 300 µm spots in subarrays (SUB-ARRAY) of 12 x 12 clones (ROW and COLUMN indicated) at 665 µm spacing on Supercharge nytran membranes (Schleicher & Schuell) using a GMS 417 arrayer (Genetic Microsystems). !Platform_description = Subarrays were printed in duplicate (DUPLICATE a or b). !Platform_description = Printing was validated (SPOT_QC) by hybridization of one array with the short vector sequence common to all amplicons (pBluescript SK, 626-791 bp). !Platform_description = SPOT_QC values are hybridization intensity. BEST-HIT and associated E value for ESTs aligned with the nr database are given. !Platform_description = Keywords = germline sex testis, membrane, filter !Platform_pubmed_id = 11116097 !Platform_contributor = B,,Oliver !Platform_contributor = J,,Andrews !Platform_contributor = G,G,Bouffard !Platform_contributor = C,,Cheadle !Platform_contributor = J,,Lu !Platform_contributor = K,G,Becker !Platform_contact_name = Brian,,Oliver !Platform_contact_email = briano@nih.gov !Platform_contact_phone = 301-204-9463 !Platform_contact_laboratory = Developmental Genomics !Platform_contact_department = LBG !Platform_contact_institute = NIDDK, NIH !Platform_contact_address = 50 South Drive !Platform_contact_city = Bethesda !Platform_contact_state = MD !Platform_contact_zip/postal_code = 20892 !Platform_contact_country = USA !Platform_sample_id = GSM3 !Platform_sample_id = GSM4 !Platform_sample_id = GSM5 !Platform_sample_id = GSM6 !Platform_sample_id = GSM7 !Platform_sample_id = GSM8 !Platform_sample_id = GSM9 !Platform_sample_id = GSM10 !Platform_series_id = GSE462 !Platform_data_row_count = 3456