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Status
Public on Jun 04, 2008
Title
MBPL human 30k P6
Technology type
spotted DNA/cDNA
Distribution
non-commercial
Organism
Homo sapiens Manufacturer
MBPL in house arrayer
Manufacture protocol
Arrayer obtained from Dr. Patrick O. Brown MGuide II and ArrayMarker program version 2.3.2 (Dr. Joseph DeRisi, university of California, San Francisco) with 48 spotting pins (TeleChem).We control printing condition in temperature 25~27°C and Humidity about 49~51%, and printing on April 2005. cDNA clones were purchased from Incyte Genomics and 7334 sequence-verified clones were amplified by PCR.We purified these 7334 clones by isopropanol precipitation and used 3×saline-sodium citrate (SSC) as spotting solution. cDNA spotted on GAPS Ⅱ coated slides (Corning Incorporated)coating of pure gamma amino propylsilane.
Support
glass
Coating
other
Description
There are 32448 spots within a array.7334 human clone were printing 4 duplicate in a array. Additionally, we printing 10 doping control genes (SpotReport™-10 Array Validation System , Stratagene) and one house-keeping gene , 5 negative control gene as quality control indicator. total 16 quality control indicator were printing 2 duplicate in each block.
Web link
http://theme.ns.nthu.edu.tw/~g864514/php/axon/ Contributor(s)
Hsu IC , Tsai M , Lin H , Cheng W , Huang C , Chang C , Hsieh Y , Chang K , Weng T , Chang C Submission date
Jun 05, 2007
Last update date
Mar 02, 2011
Contact name
ChengWei Chang
E-mail(s)
d924546@oz.nthu.edu.tw Organization name
nthu
Department
bmes
Lab
mbpl
Street address
101, Section 2 Kuang Fu Road
City
Hsinchu
ZIP/Postal code
30013
Country
Taiwan
Samples (44)
GSM198754 , GSM198960 , GSM198961 , GSM198962 , GSM198963 , GSM198964
GSM198965 ,
GSM198966 ,
GSM198967 ,
GSM198968 ,
GSM200649 ,
GSM200650 ,
GSM200651 ,
GSM200652 ,
GSM200653 ,
GSM200654 ,
GSM200655 ,
GSM200656 ,
GSM200657 ,
GSM200658 ,
GSM684925 ,
GSM684926 ,
GSM684927 ,
GSM684928 ,
GSM684929 ,
GSM684930 ,
GSM684931 ,
GSM684932 ,
GSM684933 ,
GSM684934 ,
GSM684935 ,
GSM684936 ,
GSM684937 ,
GSM684938 ,
GSM684939 ,
GSM684940 ,
GSM684941 ,
GSM684942 ,
GSM684943 ,
GSM684944 ,
GSM684945 ,
GSM684946 ,
GSM684947 ,
GSM684948 Series (2)
GSE8085 BK virus infection induces changes of cellular gene expression in Vero cell
GSE27646 Intra- and Inter-Individual Variance of Gene Expression in Human Placenta
Data table header descriptions ID BLOCK subarray (4 X 12 matix, up to 48)
COLUMN column within block(up to 26)
ROW row within block(up to 26)
SERIAL NUMBER Assignment of a number from 1 to 7334 to each cDNA clone
GB_ACC genebank accession number
UniGene unigene accession number
Name full name or description of genes
Symbol symbol of genes
GeneID GeneID of genes
ORGANISM source of organism
SPOT_ID spot identifier
Data table ID BLOCK COLUMN ROW SERIAL NUMBER GB_ACC UniGene Name Symbol GeneID ORGANISM SPOT_ID 1
1
1
1
100001
X56062 SpotReport -10 Array Validation System
Doping 1
Arabidopsis thaliana
--CONTROL: Arabidopsis thaliana
2
1
2
1
100003
U91966 SpotReport -12 Array Validation System
Doping 3
Arabidopsis thaliana
--CONTROL: Arabidopsis thaliana
3
1
3
1
100005
AF159803 SpotReport -14 Array Validation System
Doping 5
Arabidopsis thaliana
--CONTROL: Arabidopsis thaliana
4
1
4
1
100007
AF168390 SpotReport -16 Array Validation System
Doping 7
Arabidopsis thaliana
--CONTROL: Arabidopsis thaliana
5
1
5
1
100009
AF247559 SpotReport -18 Array Validation System
Doping 9
Arabidopsis thaliana
--CONTROL: Arabidopsis thaliana
6
1
6
1
100011
SpotReport -20 Array Validation System
ACTB
Homo Sapiens
--CONTROL:ACTB
7
1
7
1
100019
NM_000291 Hs.78771
5230
Homo Sapiens
8
1
8
1
100017
BF983396 Hs.544577
2597
Homo Sapiens
9
1
9
1
100018
Homo Sapiens
--UNKNOWN
10
1
10
1
100021
BC110341 Hs.590872
6908
Homo Sapiens
11
1
11
1
100020
BC030803 Hs.374147
80008
Homo Sapiens
12
1
12
1
100010
X58149 SpotReport -19 Array Validation System
Doping 10
Arabidopsis thaliana
--CONTROL: Arabidopsis thaliana
13
1
13
1
100008
AF198054 SpotReport -17 Array Validation System
Doping 8
Arabidopsis thaliana
--CONTROL: Arabidopsis thaliana
14
1
14
1
100006
AF191028 SpotReport -15 Array Validation System
Doping 6
Arabidopsis thaliana
--CONTROL: Arabidopsis thaliana
15
1
15
1
100004
AF159801 SpotReport -13 Array Validation System
Doping 4
Arabidopsis thaliana
--CONTROL: Arabidopsis thaliana
16
1
16
1
100002
X14212 SpotReport -11 Array Validation System
Doping 2
Arabidopsis thaliana
--CONTROL: Arabidopsis thaliana
17
1
17
1
4
NM_001755 Hs.460988
Core-binding factor, beta subunit
CBFB
865
Homo Sapiens
18
1
18
1
36
NM_005802 Hs.589962
Topoisomerase I binding, arginine/serine-rich
TOPORS
10210
Homo Sapiens
19
1
19
1
8
U32248 Hs.377894
Grancalcin, EF-hand calcium binding protein
GCA
25801
Homo Sapiens
20
1
20
1
40
NM_006353 Hs.236774
High mobility group nucleosomal binding domain 4
HMGN4
10473
Homo Sapiens
32448 3065 Kbytes .Supplementary data files not provided
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