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Status
Public on Nov 09, 2007
Title
UAB 32K v1
Technology type
spotted DNA/cDNA
Distribution
non-commercial
Organism
Homo sapiens Manufacturer
Howell and Elizabeth Heflin Center for Human Genetics, University of Alabama at Birmingham (UAB)
Manufacture protocol
The 32,450 BAC clones were amplified using three independent DOP PCR amplifications, followed by pooling of the DOP PCR products and re-amplification using a universal amino-modified primer. The amino-PCR-products were then purified using Millipore filter plates, eluted with printing buffer. Quality control agarose gel electrophoresis was performed for all clones after the universal amino PCR, and the gel images were stored in an image database. All DNA transfers were performed using a Hydra 96 channel micro-dispenser (Matrix Technologies, Hudson, NH) and a Biomek FX robot (Beckman-Coulter, Fullerton, CA). The aminogroup attached at the 5’ end of the universal primer, allows for covalent attachment of DNA fragments to the CodeLink HD slide surface (GE Healthcare, Piscataway, NJ). We used the spotting solution recommended for these slides (GE Healthcare). Printing of the clones was carried out using a high throughput printing robot (http://www.heflingenetics.uab.edu/CORE/array_printing.shtml ). The center-to-center distance between the spots was 160 micrometers using a hexagonal printing pattern.
Contributor(s)
Dumanski JP , Bruder CE , Piotrowski A Submission date
Nov 06, 2007
Last update date
Jan 14, 2008
Contact name
Jan P Dumanski
E-mail(s)
jdumanski@genetics.uab.edu Phone
(205) 996 4045
Organization name
University of Alabama
Department
Department of Genetics
Street address
720 20th Street South
City
Birmingham
State/province
AL
ZIP/Postal code
35244
Country
USA
Samples (133)
GSM241801 , GSM241802 , GSM241803 , GSM241804 , GSM241805 , GSM241806
GSM241807 ,
GSM241808 ,
GSM241809 ,
GSM241810 ,
GSM241811 ,
GSM241812 ,
GSM241813 ,
GSM241814 ,
GSM241815 ,
GSM241816 ,
GSM241817 ,
GSM241818 ,
GSM241819 ,
GSM241820 ,
GSM241821 ,
GSM241822 ,
GSM241823 ,
GSM241824 ,
GSM241825 ,
GSM241826 ,
GSM241827 ,
GSM241828 ,
GSM241829 ,
GSM241830 ,
GSM241831 ,
GSM241832 ,
GSM242734 ,
GSM242735 ,
GSM242736 ,
GSM242737 ,
GSM242738 ,
GSM242739 ,
GSM242740 ,
GSM242741 ,
GSM242742 ,
GSM242743 ,
GSM242744 ,
GSM242745 ,
GSM242746 ,
GSM242747 ,
GSM242748 ,
GSM242749 ,
GSM242750 ,
GSM242751 ,
GSM242752 ,
GSM242753 ,
GSM242754 ,
GSM242755 ,
GSM242756 ,
GSM242757 ,
GSM242758 ,
GSM242759 ,
GSM242760 ,
GSM242761 ,
GSM242762 ,
GSM242763 ,
GSM242764 ,
GSM242765 ,
GSM242766 ,
GSM242767 ,
GSM242768 ,
GSM242769 ,
GSM242770 ,
GSM242771 ,
GSM242772 ,
GSM242773 ,
GSM242774 ,
GSM242775 ,
GSM242776 ,
GSM242777 ,
GSM242778 ,
GSM242779 ,
GSM242780 ,
GSM242781 ,
GSM242782 ,
GSM242783 ,
GSM242784 ,
GSM242785 ,
GSM242786 ,
GSM242787 ,
GSM242788 ,
GSM242789 ,
GSM242790 ,
GSM242791 ,
GSM242792 ,
GSM242793 ,
GSM242794 ,
GSM242795 ,
GSM242796 ,
GSM242797 ,
GSM242798 ,
GSM242799 ,
GSM242800 ,
GSM242801 ,
GSM242802 ,
GSM242803 ,
GSM242804 ,
GSM242805 ,
GSM242806 ,
GSM242807 ,
GSM401425 ,
GSM401426 ,
GSM401427 ,
GSM401428 ,
GSM401429 ,
GSM401430 ,
GSM401431 ,
GSM401432 ,
GSM401433 ,
GSM401434 ,
GSM401435 ,
GSM401436 ,
GSM401437 ,
GSM401438 ,
GSM401439 ,
GSM401440 ,
GSM401441 ,
GSM401442 ,
GSM401443 ,
GSM401444 ,
GSM401445 ,
GSM401446 ,
GSM401447 ,
GSM401448 ,
GSM404714 ,
GSM404715 ,
GSM404716 Series (4)
GSE9559 Phenotypically concordant and discordant monozygotic twins display different DNA copy number variation profiles (BAC)
GSE9598 Somatic mosaicism for copy number variation in differentiated human tissues
GSE9609 Phenotypically concordant and discordant monozygotic twins display different DNA copy number variation profiles
GSE16041 Array-CGH of malignant peripheral nerve sheath tumors (MPNST)
Data table header descriptions ID block Sub-array. There are 192 sub-arrays on each slide
numCol Column in each sub-array
numRow Row in each sub-array
ReporterID BAC clone name according to CHORI 32K BAC Re-Array library
CLONE_ID GenBank Clone ID
chromosome Chromosome. Chromosome 22_B denotes clones from a separate chr. 22 library
startPosition Start position according HG17
endPosition End position according HG17
GB_RANGE Build 35 Accession version for chromosome[start..end]
SPOT_ID
Data table ID block numCol numRow ReporterID CLONE_ID chromosome startPosition endPosition GB_RANGE SPOT_ID 1
1
1
1
RP11-469D8
RP11-469D8 1
85634151
85807675
NC_000001.8[85634151..85807675]
2
1
2
1
RP11-465C20
RP11-465C20 1
76702593
76867535
NC_000001.8[76702593..76867535]
3
1
3
1
RP11-468N10
RP11-468N10 1
145894522
146153461
NC_000001.8[145894522..146153461]
4
1
4
1
RP11-464N3
RP11-464N3 1
232521388
232717181
NC_000001.8[232521388..232717181]
5
1
5
1
RP11-466J21
RP11-466J21 1
22419398
22666840
NC_000001.8[22419398..22666840]
6
1
6
1
RP11-458I7
RP11-458I7 1
146786606
146978586
NC_000001.8[146786606..146978586]
7
1
7
1
RP11-452M14
RP11-452M14 1
35681449
35886190
NC_000001.8[35681449..35886190]
8
1
8
1
RP11-452L15
RP11-452L15 1
45339801
45548646
NC_000001.8[45339801..45548646]
9
1
9
1
RP11-459O20
RP11-459O20 1
25005312
25185774
NC_000001.8[25005312..25185774]
10
1
10
1
RP11-452J13
RP11-452J13 1
189971175
190129646
NC_000001.8[189971175..190129646]
11
1
11
1
RP11-454N17
RP11-454N17 1
58838562
59028905
NC_000001.8[58838562..59028905]
12
1
12
1
RP11-457C2
RP11-457C2 1
2010680
2179451
NC_000001.8[2010680..2179451]
13
1
13
1
RP11-445O11
RP11-445O11 1
105562263
105725210
NC_000001.8[105562263..105725210]
14
2
1
1
RP11-421L10
RP11-421L10 1
217705373
217908841
NC_000001.8[217705373..217908841]
15
2
2
1
RP11-419E8
RP11-419E8 1
39968416
40180797
NC_000001.8[39968416..40180797]
16
2
3
1
RP11-423I7
RP11-423I7 1
74507391
74703372
NC_000001.8[74507391..74703372]
17
2
4
1
RP11-419B18
RP11-419B18 1
193911799
194080166
NC_000001.8[193911799..194080166]
18
2
5
1
RP11-419A24
RP11-419A24 1
44028725
44225047
NC_000001.8[44028725..44225047]
19
2
6
1
RP11-420O4
RP11-420O4 1
241716206
241930127
NC_000001.8[241716206..241930127]
20
2
7
1
RP11-417C2
RP11-417C2 1
71484329
71621881
NC_000001.8[71484329..71621881]
37440 3050 Kbytes .Supplementary data files not provided
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