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Platform GPL6284 Query DataSets for GPL6284
Status Public on Dec 20, 2007
Title INRA-BDR Bovine D14 Embryo 1K
Technology type spotted DNA/cDNA
Distribution non-commercial
Organism Bos taurus
Manufacturer INRA/UMR Biologie du Developpement et Reproduction
Manufacture protocol cDNA inserts from the bcai arrayed library (bcai library indexed in TIGR as #FJB and in NCBI as "15979") were amplified by PCR using the flanking primers used to construct this SMART-amplified library (PCRp1 primers from the Cap Finder cDNA kit, Clontech). After a short run on a 2.5% agarose gel, the probes were selected (size >= 500pb), spotted and fixed (UV light, 1 min, 1200J, twice) onto nylon N+ membranes (8cmx12cm, Amersham Biosciences) with a 5x5 pattern (BioRobotics). The whole array represents 1920 probes but the biological core of the array (1K array) represents only 987 informative probes (1097 sequences submitted to the EBI deprived of the 110 mitochondrial sequences).
Support nylon N+ membranes
 
Description (8cmx12cm, Amersham Biosciences) 5x5 pattern
 
Contributor(s) Hue I, Degrelle S, Piumi F
Submission date Dec 14, 2007
Last update date Dec 20, 2007
Contact name Isabelle Hue
E-mail(s) isabelle.hue@jouy.inra.fr
Phone 33134652567
Fax 33134652364
Organization name INRA
Department PHASE
Lab UMR Biologie du Developpement et Reproduction
Street address Domaine de Vilvert
City Jouy en Josas
ZIP/Postal code 78350
Country France
 
Samples (96) GSM251244, GSM251245, GSM251246, GSM251247, GSM251248, GSM251249 
Series (1)
GSE9929 Comparison of linear and exponential protocols using moderate amplifications

Data table header descriptions
ID
Block
X
Y
GB_ACC GenBank Accession number
SPOT_ID

Data table
ID Block X Y GB_ACC SPOT_ID
bcai0001a.a.01_5.1 1 1 1 bcai0001a.a.01_5.1
bcai0002a.a.01_5.1 1 2 1 CR450900
bcai0003a.a.01_5.1 1 3 1 bcai0003a.a.01_5.1
bcai0004a.a.01_5.1 1 4 1 CR451375
bcai0005a.a.01_5.1 1 5 1 CR451115
bcai0006a.a.01_5.1 1 1 2 bcai0006a.a.01_5.1
bcai0007a.a.01_5.1 1 2 2 CR454994
bcai0008a.a.01_5.1 1 3 2 CR451197
bcai0009a.a.01_5.1 1 4 2 CR453162
bcai0010a.a.01_5.1 1 5 2 CR453101
bcai0011a.a.01_5.1 1 1 3 CR450917
bcai0012a.a.01_5.1 1 2 3 bcai0012a.a.01_5.1
bcai0013a.a.01_5.1 1 3 3 bcai0013a.a.01_5.1
bcai0014a.a.01_5.1 1 4 3 bcai0014a.a.01_5.1
bcai0015a.a.01_5.1 1 5 3 bcai0015a.a.01_5.1
bcai0016a.a.01_5.1 1 1 4 bcai0016a.a.01_5.1
bcai0017a.a.01_5.1 1 2 4 bcai0017a.a.01_5.1
bcai0018a.a.01_5.1 1 3 4 bcai0018a.a.01_5.1
bcai0019a.a.01_5.1 1 4 4 bcai0019a.a.01_5.1
bcai0020a.a.01_5.1 1 5 4 CR451333

Total number of rows: 1920

Table truncated, full table size 76 Kbytes.




Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp

Supplementary data files not provided

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