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Status
Public on Dec 20, 2007
Title
INRA-BDR Bovine D14 Embryo 1K
Technology type
spotted DNA/cDNA
Distribution
non-commercial
Organism
Bos taurus
Manufacturer
INRA/UMR Biologie du Developpement et Reproduction
Manufacture protocol
cDNA inserts from the bcai arrayed library (bcai library indexed in TIGR as #FJB and in NCBI as "15979") were amplified by PCR using the flanking primers used to construct this SMART-amplified library (PCRp1 primers from the Cap Finder cDNA kit, Clontech). After a short run on a 2.5% agarose gel, the probes were selected (size >= 500pb), spotted and fixed (UV light, 1 min, 1200J, twice) onto nylon N+ membranes (8cmx12cm, Amersham Biosciences) with a 5x5 pattern (BioRobotics). The whole array represents 1920 probes but the biological core of the array (1K array) represents only 987 informative probes (1097 sequences submitted to the EBI deprived of the 110 mitochondrial sequences).
Support
nylon N+ membranes
Description
(8cmx12cm, Amersham Biosciences) 5x5 pattern
Contributor(s)
Hue I , Degrelle S , Piumi F
Submission date
Dec 14, 2007
Last update date
Dec 20, 2007
Contact name
Isabelle Hue
E-mail(s)
isabelle.hue@jouy.inra.fr
Phone
33134652567
Fax
33134652364
Organization name
INRA
Department
PHASE
Lab
UMR Biologie du Developpement et Reproduction
Street address
Domaine de Vilvert
City
Jouy en Josas
ZIP/Postal code
78350
Country
France
Samples (96)
GSM251244 , GSM251245 , GSM251246 , GSM251247 , GSM251248 , GSM251249
GSM251250 ,
GSM251251 ,
GSM251252 ,
GSM251253 ,
GSM251254 ,
GSM251255 ,
GSM251256 ,
GSM251257 ,
GSM251258 ,
GSM251259 ,
GSM251260 ,
GSM251261 ,
GSM251262 ,
GSM251263 ,
GSM251264 ,
GSM251265 ,
GSM251266 ,
GSM251267 ,
GSM251268 ,
GSM251269 ,
GSM251270 ,
GSM251271 ,
GSM251272 ,
GSM251273 ,
GSM251274 ,
GSM251275 ,
GSM251276 ,
GSM251277 ,
GSM251278 ,
GSM251279 ,
GSM251280 ,
GSM251281 ,
GSM251282 ,
GSM251283 ,
GSM251284 ,
GSM251285 ,
GSM251286 ,
GSM251287 ,
GSM251288 ,
GSM251289 ,
GSM251290 ,
GSM251291 ,
GSM251292 ,
GSM251293 ,
GSM251294 ,
GSM251295 ,
GSM251296 ,
GSM251297 ,
GSM251298 ,
GSM251299 ,
GSM251300 ,
GSM251301 ,
GSM251302 ,
GSM251303 ,
GSM251304 ,
GSM251305 ,
GSM251306 ,
GSM251307 ,
GSM251308 ,
GSM251309 ,
GSM251310 ,
GSM251311 ,
GSM251312 ,
GSM251313 ,
GSM251314 ,
GSM251315 ,
GSM251316 ,
GSM251317 ,
GSM251318 ,
GSM251319 ,
GSM251320 ,
GSM251321 ,
GSM251322 ,
GSM251323 ,
GSM251324 ,
GSM251325 ,
GSM251326 ,
GSM251327 ,
GSM251328 ,
GSM251329 ,
GSM251330 ,
GSM251331 ,
GSM251332 ,
GSM251333 ,
GSM251334 ,
GSM251335 ,
GSM251336 ,
GSM251337 ,
GSM251338 ,
GSM251339
Series (1)
GSE9929
Comparison of linear and exponential protocols using moderate amplifications
Data table header descriptions
ID
Block
X
Y
GB_ACC
GenBank Accession number
SPOT_ID
Data table
ID
Block
X
Y
GB_ACC
SPOT_ID
bcai0001a.a.01_5.1
1
1
1
bcai0001a.a.01_5.1
bcai0002a.a.01_5.1
1
2
1
CR450900
bcai0003a.a.01_5.1
1
3
1
bcai0003a.a.01_5.1
bcai0004a.a.01_5.1
1
4
1
CR451375
bcai0005a.a.01_5.1
1
5
1
CR451115
bcai0006a.a.01_5.1
1
1
2
bcai0006a.a.01_5.1
bcai0007a.a.01_5.1
1
2
2
CR454994
bcai0008a.a.01_5.1
1
3
2
CR451197
bcai0009a.a.01_5.1
1
4
2
CR453162
bcai0010a.a.01_5.1
1
5
2
CR453101
bcai0011a.a.01_5.1
1
1
3
CR450917
bcai0012a.a.01_5.1
1
2
3
bcai0012a.a.01_5.1
bcai0013a.a.01_5.1
1
3
3
bcai0013a.a.01_5.1
bcai0014a.a.01_5.1
1
4
3
bcai0014a.a.01_5.1
bcai0015a.a.01_5.1
1
5
3
bcai0015a.a.01_5.1
bcai0016a.a.01_5.1
1
1
4
bcai0016a.a.01_5.1
bcai0017a.a.01_5.1
1
2
4
bcai0017a.a.01_5.1
bcai0018a.a.01_5.1
1
3
4
bcai0018a.a.01_5.1
bcai0019a.a.01_5.1
1
4
4
bcai0019a.a.01_5.1
bcai0020a.a.01_5.1
1
5
4
CR451333
Total number of rows: 1920 Table truncated, full table size 76 Kbytes .
Supplementary data files not provided