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Platform GPL6474 Query DataSets for GPL6474
Status Public on Mar 14, 2008
Title Candida albicans ChIP-CHIP array
Technology type spotted oligonucleotide
Distribution non-commercial
Organism Candida albicans
Manufacturer National Research Council Canada - Biotechnology Research Institute
Manufacture protocol Probe design was done using a sliding window of 70 bp moved from the 3' -> 5' end of the target sequence. Self-annealing was calculated at each position as well as the GC count. A pre-computed cumulative mask of all the significant alignments found in the genome was made to favour regions that are unique for each genomic region.
Oligos were resuspended at 22 µM concentration in 50% DMSO and spotted on Corning Ultragaps slides with a BioRad ChipWriter Pro microarray spotter equipped with 48 SMP3 pins (Telechem ArrayIt). UV crosslinking (600 mJ) was performed in a Stratagene UV Stratalinker.
 
 
Contributor(s) Nantel A, Deneault J, Arbour M, Hogues H, Whiteway M
Submission date Feb 08, 2008
Last update date Mar 14, 2008
Contact name Hugo Lavoie
E-mail(s) hugo.lavoie@nrc-cnrc.gc.ca
Phone 514-496-6154
Fax 514-496-6213
Organization name CNRC-IRB
Department Genetics
Street address 6100 Royalmount
City Montreal
State/province Quebec
ZIP/Postal code H4P 2R2
Country Canada
 
Samples (10) GSM264698, GSM264703, GSM264705, GSM264707, GSM338661, GSM338662 
Series (3)
GSE10458 Transcription Factor Substitution during the Evolution of Fungal Ribosome Regulation_ChIP-CHIP
GSE10622 Transcription Factor Substitution during the Evolution of Fungal Ribosome Regulation
GSE13427 A toolbox for epitope-tagging and genome-wide location analysis in Candida albicans

Data table header descriptions
ID
SEQUENCE The nucleotide sequence of each oligonucleotide on the array

Data table
ID SEQUENCE
CA1000010C CGAGTCAGGGTGATTGCAAAAAAACAGAAACAATAGATAATCTTAAATTAAGGTAGTAAAGGCTCTGTGA
CA1000020W ACTTACACTGCTCTCTGATGTTCCGATGTCCAACTGCCGTCCTTGACTCCGGTTTGTCCCTAAAGATTAC
CA1000030W CTGTTCAATTCCTACCCCAAACTATTCCTTGAGTTTGTTCTTCTATTTTCTGGCCGCGGTTGTTTCAAAA
CA1000040C TAATAAGAATGTAGTGACAATGTTTAGAAGAGGGGTTTTGACTGTTAGCTACACCAAGAGTAGTAAAGCT
CA1000050C ATTAATAAGAATGTAGTGACAATGTTTAGAAGAGGGGTTTTGACTGTTAGCTACACCAAGAGTAGTAAAG
CA1000060W AACCGAAGGTATCTTCGCTACAGGTAGTGGCGATTGTAAAGCCAGAATTTGGAAATGGACCAAAAAATAA
CA1000070C TTTCTCACATATATATATACTACACACTTATTTTTTGGTCCATTTCCAAATTCTGGCTTTACAATCGCCA
CA1000080W TATCTCAACGATACGTCCAAAAAGATTATAAAAATGGTAGAAACCATCAACCAGCAAGAAGTCGTTGCTG
CA1000090C AAGAGTTGAAGAAGAAAGACGCAAAGATTGCTGAGTTGGAGTATACGGTGGAATTACTAAAAGCAAGTAT
CA1000100W TTGGTTGGGGTCAAAGAGTGGTTGTTCAAATGGTGGAAAGTTCATGGCTGGTAAAGAAAGTTGTGAGACA
CA1000110W AAGTGGAAGGGTAAATACTATTTTGGCAACACAGTAAGAAAGAGAGTCTGGATAAGACTAAGGAAGAAAT
CA1000120C TAGCAAGCATAATAATTGGCCGCGATGTGATGTTGAGTTTTATGTCGTTCTACTATAGGTACAAGAGCTT
CA1000130W TCTTGTACCTATAGTAGAACGACATAAAACTCAACATCACATCGCGGCCAATTATTATGCTTGCTATCAC
CA1000140W CTTGGCTACAAAGAAATCCGCTATAGATTTAGCTGTTGATGCTACAAACACTATCTTATCTATAGATCAA
CA1000150C CAACATTTTGGGCTACGATGTTAGTGGCGGTGTTGAAACCAATGGGGTAAAAGATTCATTGAAAATTATA
CA1000160W GCAACACTCGCTGCCTCCACCGTCTTGATACCACAAATCTTAACTACTTTCATTACCTATATCTTTACGG
CA1000170C GCCGCGACCCCGGATGTGAAGTTGAATGAAAAATTTGATGTGAGCAAGATAAACGAGAATTTTAAAAATT
CA1000180W AAAACTATACCCATAACCAATTGACAGAATCTCGACCCATTTGGGAACCCCTAAAGTCTATTTTATTTCT
CA1000190W TACCAGTAAGGCATTGAAATTGAACCAAGAAGAGTTAAGCAACCAACGAATTCTAATGGAAAGATTGATT
CA1000200C AAATTACTTGAACGTCAACAATGACCGTTTGATAGTTCCCCAGGATAATAACTCGAATTCAGAGACGTAA

Total number of rows: 11817

Table truncated, full table size 946 Kbytes.




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Supplementary data files not provided
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