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Platform GPL6475 Query DataSets for GPL6475
Status Public on Mar 14, 2008
Title Candida albicans expression array
Technology type spotted oligonucleotide
Distribution non-commercial
Organism Candida albicans
Manufacturer National Research Council Canada - Biotechnology Research Institute
Manufacture protocol Probe design was done using a sliding window of 70 bp moved from the 3' -> 5' end of the target sequence. Self-annealing was calculated at each position as well as the GC count. A pre-computed cumulative mask of all the significant alignments found in the genome was made to favour regions that are unique for each genomic region.
Oligos were resuspended at 22 µM concentration in 50% DMSO and spotted on Corning Ultragaps slides with a BioRad ChipWriter Pro microarray spotter equipped with 48 SMP3 pins (Telechem ArrayIt). UV crosslinking (600 mJ) was performed in a Stratagene UV Stratalinker.
 
 
Contributor(s) Nantel A, Deneault J, Arbour M, Hogues H, Whiteway M
Submission date Feb 08, 2008
Last update date Mar 14, 2008
Contact name Hugo Lavoie
E-mail(s) hugo.lavoie@nrc-cnrc.gc.ca
Phone 514-496-6154
Fax 514-496-6213
Organization name CNRC-IRB
Department Genetics
Street address 6100 Royalmount
City Montreal
State/province Quebec
ZIP/Postal code H4P 2R2
Country Canada
 
Samples (18) GSM265362, GSM265364, GSM265365, GSM265367, GSM265368, GSM265370 
Series (4)
GSE10499 Transcription Factor Substitution during the Evolution of Fungal Ribosome Regulation_expression profiling
GSE10622 Transcription Factor Substitution during the Evolution of Fungal Ribosome Regulation
GSE13318 Expression profiling of Candida albicans and Candida dubliniensis in reconstituted human oral epithelium 30 min p.i.

Data table header descriptions
ID
SEQUENCE The nucleotide sequence of each oligonucleotide on the array

Data table
ID SEQUENCE
orf19.5 TGGTTATGAGACAACTGTGACTATTTGCTCTGATGTTACTTCTGGACAAAGTGTTACCACTCCTACTACC
orf19.6 AACGTTCGCCCTCCACTCCATTTCTGTAGCTATATCAACCGCCGCCTGTGTTTCCATCCTCCTTTCAGTA
orf19.7 TAATCAACTGAATATCTTCTCGTTTGTCCTGTCTCCCATCACTGTTCTGTCATCTTCCTGCTTTCCTCAA
orf19.8 GGCAACAACGATCAAGAAATTCAAACTCAGGCAATTCGACAATTACATGATAAATTAAGGTCACAAAAAG
orf19.9 ATAATACTTATGCCACTCATGAAATATGGCAAATGAGACGTGGAGTTGGTGCTATTATTATCATTTCTAC
orf19.10 TTTGCAATTGACAGAAGGTATAGAATATCCTCCAGCTAAGTTGACTCATTTAACAATGTGTATGTTTGAT
orf19.11 TGCTGAAGAAGAGAAGAAGGTTGACTCGTATGACAACAATAAGGTTCCACAAGTACCCACACAGGACTAA
orf19.13 CAGAATTGAAGAGCCATGGTTGTTGGATGCTGAAGTGTTGTCTTATCTCCAGATTGATGACTCCACTGAT
orf19.14 TCAACAATGTTTACCAGAAAGAAATGAGCGGTGTTCTATTTTGGGGGTATGTTGTTTTGGTAGTACCAAC
orf19.15 AGAAAGAGAATTGGGTGTGAGATGATCAGCGAGACGGCAAGAATGATAATAGAAGAAAGCACTACTGGGA
orf19.16 TCTTAATTGTAGTCTCCGTCCTCCCTTCTCTATTCACTCTCATACGGCTTTCGTCTCTTTTCACAATCAT
orf19.17 AGTCATTGGTCCTAAAATTGTTAAAGTGAAACCAACAGTGCCGATAAAACCCTATAAATTAAGAGCATAG
orf19.18 CAATTGACGAATTGAGAGAAAACGTTGATAATGGTGAAATTAGATTTGAATTTAGAACTGCTTCAGCTCA
orf19.20 TCATTAGCAGAACAAAAACAACGTGAAGCAAATAGAAAAGAAGGATGGAAACGAGTTGAAGAATTAGTTC
orf19.21 CAAAGTTGTATGGACAAGAAATGCTCAAAAACCTCAATATCAACGATCAATGTCGACTCTATTACGAGTA
orf19.22 CATTTGCGTATTGTGGCACTGAACATTTGGGCTACTGGTTGGAATGGATTTTTAAGTTTTGTCCATAATA
orf19.23 TTAGTGACAATGAAGAATCGACGTTGCAAGGTCAAAATATTGTTAGGGGTGATCCTATTCAAGAGAATAA
orf19.24 ATGATGTTGAATCACAAGAACGCAGTTCTAGCGTGGTTGCAAATGACATACATTGTTTAAATAGTAAGTT
orf19.25 TAACAGTAACAGTAACACCACTAATTCAACAGACACGATCAACACTGAAAATTTGGTATATCTCACCGCT
orf19.26 AACAAGGAAATTAACCAATTGAAACCAGAATTAACAAAAACTACACCATCCACGGATTTACCACTGGGTA

Total number of rows: 6320

Table truncated, full table size 505 Kbytes.




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Supplementary data files not provided
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