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Status
Public on Mar 29, 2008
Title
AppChip2 (NRC-IBS Actinobacillus pleuropneumoniae L20)
Technology type
spotted DNA/cDNA
Distribution
non-commercial
Organism
Actinobacillus pleuropneumoniae serovar 5b str. L20 Manufacturer
NRC-BRI
Manufacture protocol
DNA microarrayer (Bio-Rad) equipped with a 48-pin print-heads that uses "quill" pin technology (TeleChem / ArrayIt).
Description
PCR primers were designed for 1954 ORFs from the completed genomic sequence of Actinobacillus pleuropneumoniae L20 which comprised 2012 ORFs. Good amplicons of length greater than 160 nt were obtained for 1846 ORFs representing 92% coverage of ORFs. All amplicons are spotted in duplicate on the microarray and sheared total genomic DNA from A. pleuropneumoniae L20 and porcine DNA were spotted in the bottom row of each subarray as controls.
Web link
http://ibs-isb.nrc-cnrc.gc.ca/glycobiology/appchips_e.html Contributor(s)
Findlay WA , Agnew B , Foote S , Nash JH Submission date
Mar 27, 2008
Last update date
Mar 29, 2008
Contact name
John H Nash
E-mail(s)
John.Nash@gmail.com Organization name
National Research Council
Department
Institute for Biological Sciences
Street address
100 Sussex Drive
City
Ottawa
State/province
Ontario
ZIP/Postal code
K1A0R6
Country
Canada
Samples (145)
GSM301267 , GSM301268 , GSM301269 , GSM301270 , GSM301271 , GSM301272
GSM301273 ,
GSM301274 ,
GSM301275 ,
GSM301276 ,
GSM301277 ,
GSM301278 ,
GSM301279 ,
GSM301280 ,
GSM301281 ,
GSM301282 ,
GSM301283 ,
GSM301284 ,
GSM301285 ,
GSM301286 ,
GSM301287 ,
GSM301288 ,
GSM301289 ,
GSM301290 ,
GSM301291 ,
GSM301292 ,
GSM301293 ,
GSM301294 ,
GSM301295 ,
GSM301296 ,
GSM301297 ,
GSM301298 ,
GSM301299 ,
GSM325785 ,
GSM325846 ,
GSM325847 ,
GSM325848 ,
GSM325849 ,
GSM325900 ,
GSM325901 ,
GSM325902 ,
GSM325903 ,
GSM325904 ,
GSM325905 ,
GSM325927 ,
GSM325928 ,
GSM325929 ,
GSM325930 ,
GSM325931 ,
GSM325932 ,
GSM365414 ,
GSM365415 ,
GSM365416 ,
GSM365417 ,
GSM365418 ,
GSM365419 ,
GSM365420 ,
GSM365421 ,
GSM365422 ,
GSM365423 ,
GSM365424 ,
GSM365425 ,
GSM365426 ,
GSM365427 ,
GSM365428 ,
GSM365429 ,
GSM365430 ,
GSM365431 ,
GSM365432 ,
GSM365433 ,
GSM365434 ,
GSM365435 ,
GSM365436 ,
GSM365437 ,
GSM365438 ,
GSM365439 ,
GSM365440 ,
GSM365441 ,
GSM365442 ,
GSM365443 ,
GSM365444 ,
GSM365445 ,
GSM365446 ,
GSM365447 ,
GSM365448 ,
GSM365449 ,
GSM365450 ,
GSM365451 ,
GSM365452 ,
GSM365453 ,
GSM365454 ,
GSM365455 ,
GSM365456 ,
GSM365457 ,
GSM365458 ,
GSM365459 ,
GSM365460 ,
GSM365461 ,
GSM365462 ,
GSM365463 ,
GSM365464 ,
GSM365465 ,
GSM365466 ,
GSM365467 ,
GSM365468 ,
GSM365469 ,
GSM365470 ,
GSM365471 ,
GSM365472 ,
GSM365473 ,
GSM365474 ,
GSM365475 ,
GSM365476 ,
GSM365477 ,
GSM365478 ,
GSM365479 ,
GSM365480 ,
GSM365481 ,
GSM365482 ,
GSM365483 ,
GSM365484 ,
GSM365485 ,
GSM365486 ,
GSM365487 ,
GSM399435 ,
GSM399436 ,
GSM399461 ,
GSM399462 ,
GSM399463 ,
GSM1071701 ,
GSM1071702 ,
GSM1071703 ,
GSM1071704 ,
GSM1071705 ,
GSM1071706 ,
GSM1071707 ,
GSM1071708 ,
GSM1071709 ,
GSM1071710 ,
GSM1071711 ,
GSM1071712 ,
GSM1071713 ,
GSM1071714 ,
GSM1071715 ,
GSM1071716 Series (10)
GSE11921 Microarray-based comparative genomic profiling of reference strains of Actinobacillus pleuropneumoniae
GSE13006 Transcript profiling of Actinobacillus pleuropneumoniae in BALF
GSE13116 Actinobacillus pleuropneumoniae BHI vs Koromycin
GSE14639 Comparative genomic profiling of reference strains and selected Canadian field isolates of A. pleuropneumoniae
GSE15911 Actinobacillus pleuropneumoniae during a natural infection in pigs
GSE43820 Transcriptome of 4h static biofilm Actinobacillus pleuropneumoniae
GSE43821 Transcriptome of 6h static biofilm Actinobacillus pleuropneumoniae
GSE43822 Comparison between the transcriptomes of 4h static biofilm and a 6h static biofilm of Actinobacillus pleuropneumoniae
GSE43823 Characterization of the transcriptomes Actinobacillus pleuropneumoniae drip flow biofilm dripflow biofilm
GSE43824 Transcriptome of A. pleuropneumoniae biofilms cultured under different growth conditions
Data table header descriptions ID ReporterID Unique ID from Reporter (used internally)
ReporterName Name of Reporter (original ORF name)
ORF ORF identifier submitted to GenBank
ORF Gene name
ArrayY Array row on chip
ArrayX Array column on chip
SpotY Spot row in subarray
SpotX Spot column in subarray
Block Block on microarray
QC Quality of amplicon
AmpSize Size of amplicon
SPOT_ID
5376 300 Kbytes .Supplementary data files not provided
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