NIH Clinical Center, Dept. of Transfusion Medicine, Immunogenetics Section (NIH/CC/DTM)
Manufacture protocol
Microarrays are printed with GeneMachines robotic arrayer from Genomic Solutions (http://www.genomicsolutions.com) that is fitted with a printhead containing 32 SMP2.5 printing pins from Telechem International (http://www.arrayit.com). The human cDNA probe set is based on RG_HsKG_031901 8k clone set and 9k clones from the RG_Hs_seq_ver_070700 40k clone set from Research Genetics (http://clones.invitrogen.com). The set includes 12,072 uniquely named genes, 875 duplicates of named genes and all remainder consisted of expression sequence tags (about 4053 EST). In addition, 194 selected cDNA clones from Stratgene and 218 custom designed 60mer oligo representing transcripts with immunological function were added to the probe set. The cDNA probes were amplified by PCR and distributed into 384-well polypropylene plates (Genetix, 384 micro array) and dissolved in 3XSSC print buffer at a concentration of 50-100 ug/ml. The microarray spot densities are 280 microns (center-to-center), and one spot is printed per probe per slide. The microarrays are printed on Fisher Premium microscope slides (cat# 12-544-1) coated with poly-L-lysine and post-processed according to the procedures on the NCI website http://madb.nci.nih.gov/.
Support
glass
Coating
polylysine
Description
Derived from GPL5959 07/01/09 - CCDTM Hs_CCDTM17.5k version 2
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