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Status
Public on Jan 03, 2012
Title
Allergochip for asthma diagnosis
Technology type
spotted peptide or protein
Distribution
non-commercial
Organism
Dermatophagoides pteronyssinus
Manufacturer
Crisanti's Lab-Imperial College
Manufacture protocol
The array contained 103 allergens printed in duplicate onto aldehyde-activated glass microscope slides (CEL Associates) using high-speed robotics (Microgrid Compact; Biorobotics). Arrays were printed at 23°C/60°C humidity and stored overnight inside the printing cabinet. Allergens (provided from Allergopharma) were initially reconstituted in PBS pH7.4 (reconstitution buffer) with a final concentration ranging form 0.4 to 40 mg/ml and after that spotted onto the arrays in the following spotting buffer PBS pH7.4, glycine pH2.4, Borate pH9.4, glycerol 10%, DTT 5mM, SDS (0.2%; 0.05%), Tween 20 (0.01%). Allergens spotting concentration ranged from 0.008 to 3 mg/ml. Goat anti-human IgE was spotted on 4 spots for positive control. Spotting buffer was printed on 46 spots with the aim of negative control. Internal calibration curve included 7 spots (duplicate). 103 spot data (duplicate) were printed on the array. Therefore, a total of 270 spot were included in the array. A detailed description of the sample (allergens, stock and spotting concentration, spotting buffer) is provided upon request.
Submission date
Jan 24, 2010
Last update date
Jan 03, 2012
Contact name
tania dottorini
Organization name
University of Perugia
Department
Dept. Exp.Medicine and Bichem. Sci.
Street address
via del Giochetto
City
Perugia
ZIP/Postal code
06126
Country
Italy
Samples (872)
GSM499992 , GSM499993 , GSM499994 , GSM499995 , GSM499996 , GSM499997
GSM499998 ,
GSM499999 ,
GSM500000 ,
GSM500001 ,
GSM500002 ,
GSM500003 ,
GSM500004 ,
GSM500005 ,
GSM500006 ,
GSM500007 ,
GSM500008 ,
GSM500009 ,
GSM500010 ,
GSM500011 ,
GSM500012 ,
GSM500013 ,
GSM500014 ,
GSM500015 ,
GSM500016 ,
GSM500017 ,
GSM500018 ,
GSM500019 ,
GSM500020 ,
GSM500021 ,
GSM500022 ,
GSM500023 ,
GSM500024 ,
GSM500025 ,
GSM500026 ,
GSM500027 ,
GSM500028 ,
GSM500029 ,
GSM500030 ,
GSM500031 ,
GSM500032 ,
GSM500033 ,
GSM500034 ,
GSM500035 ,
GSM500036 ,
GSM500037 ,
GSM500038 ,
GSM500039 ,
GSM500040 ,
GSM500041 ,
GSM500042 ,
GSM500043 ,
GSM500044 ,
GSM500045 ,
GSM500046 ,
GSM500047 ,
GSM500048 ,
GSM500049 ,
GSM500050 ,
GSM500051 ,
GSM500052 ,
GSM500053 ,
GSM500054 ,
GSM500055 ,
GSM500056 ,
GSM500057 ,
GSM500058 ,
GSM500059 ,
GSM500060 ,
GSM500061 ,
GSM500062 ,
GSM500063 ,
GSM500064 ,
GSM500065 ,
GSM500066 ,
GSM500067 ,
GSM500068 ,
GSM500069 ,
GSM500070 ,
GSM500071 ,
GSM500072 ,
GSM500073 ,
GSM500074 ,
GSM500075 ,
GSM500076 ,
GSM500077 ,
GSM500078 ,
GSM500079 ,
GSM500080 ,
GSM500081 ,
GSM500082 ,
GSM500083 ,
GSM500084 ,
GSM500085 ,
GSM500086 ,
GSM500087 ,
GSM500088 ,
GSM500089 ,
GSM500090 ,
GSM500091 ,
GSM500092 ,
GSM500093 ,
GSM500094 ,
GSM500095 ,
GSM500096 ,
GSM500097 ,
GSM500098 ,
GSM500099 ,
GSM500100 ,
GSM500101 ,
GSM500102 ,
GSM500103 ,
GSM500104 ,
GSM500105 ,
GSM500106 ,
GSM500107 ,
GSM500108 ,
GSM500109 ,
GSM500110 ,
GSM500111 ,
GSM500112 ,
GSM500113 ,
GSM500114 ,
GSM500115 ,
GSM500116 ,
GSM500117 ,
GSM500118 ,
GSM500119 ,
GSM500120 ,
GSM500121 ,
GSM500122 ,
GSM500123 ,
GSM500124 ,
GSM500125 ,
GSM500126 ,
GSM500127 ,
GSM500128 ,
GSM500129 ,
GSM500130 ,
GSM500131 ,
GSM500132 ,
GSM500133 ,
GSM500134 ,
GSM500135 ,
GSM500136 ,
GSM500137 ,
GSM500138 ,
GSM500139 ,
GSM500140 ,
GSM500141 ,
GSM500142 ,
GSM500143 ,
GSM500144 ,
GSM500145 ,
GSM500146 ,
GSM500147 ,
GSM500148 ,
GSM500149 ,
GSM500150 ,
GSM500151 ,
GSM500152 ,
GSM500153 ,
GSM500154 ,
GSM500155 ,
GSM500156 ,
GSM500157 ,
GSM500158 ,
GSM500159 ,
GSM500160 ,
GSM500161 ,
GSM500162 ,
GSM500163 ,
GSM500164 ,
GSM500165 ,
GSM500166 ,
GSM500167 ,
GSM500168 ,
GSM500169 ,
GSM500170 ,
GSM500171 ,
GSM500172 ,
GSM500173 ,
GSM500174 ,
GSM500175 ,
GSM500176 ,
GSM500177 ,
GSM500178 ,
GSM500179 ,
GSM500180 ,
GSM500181 ,
GSM500182 ,
GSM500183 ,
GSM500184 ,
GSM500185 ,
GSM500186 ,
GSM500187 ,
GSM500188 ,
GSM500189 ,
GSM500190 ,
GSM500191 ,
GSM500192 ,
GSM500193 ,
GSM500194 ,
GSM500195 ,
GSM500196 ,
GSM500197 ,
GSM500198 ,
GSM500199 ,
GSM500200 ,
GSM500201 ,
GSM500202 ,
GSM500203 ,
GSM500204 ,
GSM500205 ,
GSM500206 ,
GSM500207 ,
GSM500208 ,
GSM500209 ,
GSM500210 ,
GSM500211 ,
GSM500212 ,
GSM500213 ,
GSM500214 ,
GSM500215 ,
GSM500216 ,
GSM500217 ,
GSM500218 ,
GSM500219 ,
GSM500220 ,
GSM500221 ,
GSM500222 ,
GSM500223 ,
GSM500224 ,
GSM500225 ,
GSM500226 ,
GSM500227 ,
GSM500228 ,
GSM500229 ,
GSM500230 ,
GSM500231 ,
GSM500232 ,
GSM500233 ,
GSM500234 ,
GSM500235 ,
GSM500236 ,
GSM500237 ,
GSM500238 ,
GSM500239 ,
GSM500240 ,
GSM500241 ,
GSM500242 ,
GSM500243 ,
GSM500244 ,
GSM500245 ,
GSM500246 ,
GSM500247 ,
GSM500248 ,
GSM500249 ,
GSM500250 ,
GSM500251 ,
GSM500252 ,
GSM500253 ,
GSM500254 ,
GSM500255 ,
GSM500256 ,
GSM500257 ,
GSM500258 ,
GSM500259 ,
GSM500260 ,
GSM500261 ,
GSM500262 ,
GSM500263 ,
GSM500264 ,
GSM500265 ,
GSM500266 ,
GSM500267 ,
GSM500268 ,
GSM500269 ,
GSM500270 ,
GSM500271 ,
GSM500272 ,
GSM500273 ,
GSM500274 ,
GSM500275 ,
GSM500276 ,
GSM500277 ,
GSM500278 ,
GSM500279 ,
GSM500280 ,
GSM500281 ,
GSM500282 ,
GSM500283 ,
GSM500284 ,
GSM500285 ,
GSM500286 ,
GSM500287 ,
GSM500288 ,
GSM500289 ,
GSM500290 ,
GSM500291 ,
GSM500292 ,
GSM500293 ,
GSM500294 ,
GSM500295 ,
GSM500296 ,
GSM500297 ,
GSM500298 ,
GSM500299 ,
GSM500300 ,
GSM500301 ,
GSM500302 ,
GSM500303 ,
GSM500304 ,
GSM500305 ,
GSM500306 ,
GSM500307 ,
GSM500308 ,
GSM500309 ,
GSM500310 ,
GSM500311 ,
GSM500312 ,
GSM500313 ,
GSM500314 ,
GSM500315 ,
GSM500316 ,
GSM500317 ,
GSM500318 ,
GSM500319 ,
GSM500320 ,
GSM500321 ,
GSM500322 ,
GSM500323 ,
GSM500324 ,
GSM500325 ,
GSM500326 ,
GSM500327 ,
GSM500328 ,
GSM500329 ,
GSM500330 ,
GSM500331 ,
GSM500332 ,
GSM500333 ,
GSM500334 ,
GSM500335 ,
GSM500336 ,
GSM500337 ,
GSM500338 ,
GSM500339 ,
GSM500340 ,
GSM500341 ,
GSM500342 ,
GSM500343 ,
GSM500344 ,
GSM500345 ,
GSM500346 ,
GSM500347 ,
GSM500348 ,
GSM500349 ,
GSM500350 ,
GSM500351 ,
GSM500352 ,
GSM500353 ,
GSM500354 ,
GSM500355 ,
GSM500356 ,
GSM500357 ,
GSM500358 ,
GSM500359 ,
GSM500360 ,
GSM500361 ,
GSM500362 ,
GSM500363 ,
GSM500364 ,
GSM500365 ,
GSM500366 ,
GSM500367 ,
GSM500368 ,
GSM500369 ,
GSM500370 ,
GSM500371 ,
GSM500372 ,
GSM500373 ,
GSM500374 ,
GSM500375 ,
GSM500376 ,
GSM500377 ,
GSM500378 ,
GSM500379 ,
GSM500380 ,
GSM500381 ,
GSM500382 ,
GSM500383 ,
GSM500384 ,
GSM500385 ,
GSM500386 ,
GSM500387 ,
GSM500388 ,
GSM500389 ,
GSM500390 ,
GSM500391 ,
GSM500392 ,
GSM500393 ,
GSM500394 ,
GSM500395 ,
GSM500396 ,
GSM500397 ,
GSM500398 ,
GSM500399 ,
GSM500400 ,
GSM500401 ,
GSM500402 ,
GSM500403 ,
GSM500404 ,
GSM500405 ,
GSM500406 ,
GSM500407 ,
GSM500408 ,
GSM500409 ,
GSM500410 ,
GSM500411 ,
GSM500412 ,
GSM500413 ,
GSM500414 ,
GSM500415 ,
GSM500416 ,
GSM500417 ,
GSM500418 ,
GSM500419 ,
GSM500420 ,
GSM500421 ,
GSM500422 ,
GSM500423 ,
GSM500424 ,
GSM500425 ,
GSM500426 ,
GSM500427 ,
GSM500428 ,
GSM500429 ,
GSM500430 ,
GSM500431 ,
GSM500432 ,
GSM500433 ,
GSM500434 ,
GSM500435 ,
GSM500436 ,
GSM500437 ,
GSM500438 ,
GSM500439 ,
GSM500440 ,
GSM500441 ,
GSM500442 ,
GSM500443 ,
GSM500444 ,
GSM500445 ,
GSM500446 ,
GSM500447 ,
GSM500448 ,
GSM500449 ,
GSM500450 ,
GSM500451 ,
GSM500452 ,
GSM500453 ,
GSM500454 ,
GSM500455 ,
GSM500456 ,
GSM500457 ,
GSM500458 ,
GSM500459 ,
GSM500460 ,
GSM500461 ,
GSM500462 ,
GSM500463 ,
GSM500464 ,
GSM500465 ,
GSM500466 ,
GSM500467 ,
GSM500468 ,
GSM500469 ,
GSM500470 ,
GSM500471 ,
GSM500472 ,
GSM500473 ,
GSM500474 ,
GSM500475 ,
GSM500476 ,
GSM500477 ,
GSM500478 ,
GSM500479 ,
GSM500480 ,
GSM500481 ,
GSM500482 ,
GSM500483 ,
GSM500484 ,
GSM500485 ,
GSM500486 ,
GSM500487 ,
GSM500488 ,
GSM500489 ,
GSM500490 ,
GSM500491 ...
Accession list truncated, click here to browse through all related public accessions You can also download a list of all accessions here
Series (1)
GSE20020
IgE reactivity profiling in an asthma affected cohort
Data table header descriptions
ID
Allergen names
Allergen names
Source
Source
Allergen class
Allergen class
Organism Scientific name
Organism Scientific name
PT_ACC
PT_ACC
SPOT_ID
Data table
ID
Allergen names
Source
Allergen class
Organism Scientific name
PT_ACC
SPOT_ID
C1
Penicillin G
crude extract
Drugs
N.A.
allergen: Penicillin G crude extract
C2
Penicillin V
crude extract
Drugs
N.A.
allergen: Penicillin V crude extract
C214
Amoxicillin
crude extract
Drugs
N.A.
allergen: Amoxicillin crude extract
D01
Dermatophagoides pteronyssinus
crude extract
Mites
Dermatophagoides pteronyssinus
allergen: Dermatophagoides pteronyssinus crude extract
D02
Dermatophagoides farinae
crude extract
Mites
Dermatophagoides farinae
allergen: Dermatophagoides farinae crude extract
D03
Dermatophagoides microceras
crude extract
Mites
Dermatophagoides microceras
allergen: Dermatophagoides microceras crude extract
D70
Acarus siro
crude extract
Mites
Acarus siro
allergen: Acarus siro crude extract
D71
Lepidoglyphus destructor
crude extract
Mites
Lepidoglyphus destructor
allergen: Lepidoglyphus destructor crude extract
D72
Tyrophagus putrescentiae
crude extract
Mites
Tyrophagus putrescentiae
allergen: Tyrophagus putrescentiae crude extract
D73
Glyciphagus domesticus
crude extract
Mites
Glyciphagus domesticus
allergen: Glyciphagus domesticus crude extract
E01
Cat epithelia
crude extract
Animal epithelia
Felis domesticus
allergen: Cat epithelia crude extract
E02
Dog epithelia
crude extract
Animal epithelia
Canis familiaris
allergen: Dog epithelia crude extract
E03
Horse epithelia
crude extract
Animal epithelia
Equus caballus
allergen: Horse epithelia crude extract
E06
Guinea pig epithelia
crude extract
Animal epithelia
Cavia porcellus
allergen: Guinea pig epithelia crude extract
E78
Budgerigar feathers
crude extract
Animal epithelia
Melopsittacus undulatus
allergen: Budgerigar feathers crude extract
E81
Sheep epithelia
crude extract
Animal epithelia
Ovis aries
allergen: Sheep epithelia crude extract
E82
Rabbit epithelia
crude extract
Animal epithelia
Oryctolagus cuniculus
allergen: Rabbit epithelia crude extract
F01
Egg white
crude extract
Food allergens
Gallus gallus
allergen: Egg white crude extract
F02
Cow's milk
crude extract
Food allergens
Bos taurus
allergen: Cow's milk crude extract
F03
Cod
crude extract
Food allergens
Gadus callarias
allergen: Cod crude extract
Total number of rows: 103 Table truncated, full table size 9 Kbytes .
Supplementary data files not provided