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Platform GPL9968 Query DataSets for GPL9968
Status Public on Jan 03, 2012
Title Allergochip for asthma diagnosis
Technology type spotted peptide or protein
Distribution non-commercial
Organism Dermatophagoides pteronyssinus
Manufacturer Crisanti's Lab-Imperial College
Manufacture protocol The array contained 103 allergens printed in duplicate onto aldehyde-activated glass microscope slides (CEL Associates) using high-speed robotics (Microgrid Compact; Biorobotics). Arrays were printed at 23°C/60°C humidity and stored overnight inside the printing cabinet. Allergens (provided from Allergopharma) were initially reconstituted in PBS pH7.4 (reconstitution buffer) with a final concentration ranging form 0.4 to 40 mg/ml and after that spotted onto the arrays in the following spotting buffer PBS pH7.4, glycine pH2.4, Borate pH9.4, glycerol 10%, DTT 5mM, SDS (0.2%; 0.05%), Tween 20 (0.01%). Allergens spotting concentration ranged from 0.008 to 3 mg/ml.
Goat anti-human IgE was spotted on 4 spots for positive control. Spotting buffer was printed on 46 spots with the aim of negative control. Internal calibration curve included 7 spots (duplicate). 103 spot data (duplicate) were printed on the array. Therefore, a total of 270 spot were included in the array.
A detailed description of the sample (allergens, stock and spotting concentration, spotting buffer) is provided upon request.
 
 
Submission date Jan 24, 2010
Last update date Jan 03, 2012
Contact name tania dottorini
Organization name University of Perugia
Department Dept. Exp.Medicine and Bichem. Sci.
Street address via del Giochetto
City Perugia
ZIP/Postal code 06126
Country Italy
 
Samples (872) GSM499992, GSM499993, GSM499994, GSM499995, GSM499996, GSM499997 
Series (1)
GSE20020 IgE reactivity profiling in an asthma affected cohort

Data table header descriptions
ID
Allergen names Allergen names
Source Source
Allergen class Allergen class
Organism Scientific name Organism Scientific name
PT_ACC PT_ACC
SPOT_ID

Data table
ID Allergen names Source Allergen class Organism Scientific name PT_ACC SPOT_ID
C1 Penicillin G crude extract Drugs N.A. allergen: Penicillin G crude extract
C2 Penicillin V crude extract Drugs N.A. allergen: Penicillin V crude extract
C214 Amoxicillin crude extract Drugs N.A. allergen: Amoxicillin crude extract
D01 Dermatophagoides pteronyssinus crude extract Mites Dermatophagoides pteronyssinus allergen: Dermatophagoides pteronyssinus crude extract
D02 Dermatophagoides farinae crude extract Mites Dermatophagoides farinae allergen: Dermatophagoides farinae crude extract
D03 Dermatophagoides microceras crude extract Mites Dermatophagoides microceras allergen: Dermatophagoides microceras crude extract
D70 Acarus siro crude extract Mites Acarus siro allergen: Acarus siro crude extract
D71 Lepidoglyphus destructor crude extract Mites Lepidoglyphus destructor allergen: Lepidoglyphus destructor crude extract
D72 Tyrophagus putrescentiae crude extract Mites Tyrophagus putrescentiae allergen: Tyrophagus putrescentiae crude extract
D73 Glyciphagus domesticus crude extract Mites Glyciphagus domesticus allergen: Glyciphagus domesticus crude extract
E01 Cat epithelia crude extract Animal epithelia Felis domesticus allergen: Cat epithelia crude extract
E02 Dog epithelia crude extract Animal epithelia Canis familiaris allergen: Dog epithelia crude extract
E03 Horse epithelia crude extract Animal epithelia Equus caballus allergen: Horse epithelia crude extract
E06 Guinea pig epithelia crude extract Animal epithelia Cavia porcellus allergen: Guinea pig epithelia crude extract
E78 Budgerigar feathers crude extract Animal epithelia Melopsittacus undulatus allergen: Budgerigar feathers crude extract
E81 Sheep epithelia crude extract Animal epithelia Ovis aries allergen: Sheep epithelia crude extract
E82 Rabbit epithelia crude extract Animal epithelia Oryctolagus cuniculus allergen: Rabbit epithelia crude extract
F01 Egg white crude extract Food allergens Gallus gallus allergen: Egg white crude extract
F02 Cow's milk crude extract Food allergens Bos taurus allergen: Cow's milk crude extract
F03 Cod crude extract Food allergens Gadus callarias allergen: Cod crude extract

Total number of rows: 103

Table truncated, full table size 9 Kbytes.




Download family Format
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Supplementary data files not provided

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