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Series GSE100037 Query DataSets for GSE100037
Status Public on Apr 09, 2018
Title Single cell RNA sequencing data of bone marrow lymphoid progenitors
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Using different surface markers it has been possible to isolate lymphoid lineage-biased progentors and test their potential in vivo and in vitro. Here we apply single cell sequencing of lymphoid progenitors to obtain further insights into differentiation and commitment to the lymphoid lineage.
 
Overall design Single cells from the bone marrow from various stages during lymphoid differentiation were sorted into 384-well plates based on their surface marker expression of Flt3, Sca-1 and c-Kit and processed using a modified version of the CEL-Seq2 protocol (Hashimshony et al. 2016, Genome Biology, DOI: 10.1186/s13059-016-0938-8). In addition the original version of the CEL-Seq2 protoco and thel modified versions with different volume reductions and were compared using murine embryonic stem cells.
 
Contributor(s) Herman JS, Sagar , Grün D
Citation(s) 29630061
Submission date Jun 14, 2017
Last update date Jul 25, 2021
Contact name Josip Stefan Herman
E-mail(s) herman@ie-freiburg.mpg.de
Phone 00497615108725
Organization name Max Planck Institute of Immunobiology and Epigenetics
Department Quantitative Single Cell Biology
Lab Research Group Dominic Grün
Street address Stuebeweg 51
City Freiburg im Breisgau
ZIP/Postal code D-79108
Country Germany
 
Platforms (2)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (39)
GSM2668205 LSK1
GSM2668206 LSK2
GSM2668207 LMPP1_1
Relations
BioProject PRJNA390541
SRA SRP109218

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE100037_RAW.tar 19.5 Mb (http)(custom) TAR (of CSV)
GSE100037_cellbarcodes_cellid.csv.gz 17.7 Kb (ftp)(http) CSV
GSE100037_cellbarcodes_cellid_culture_experiment_2.csv.gz 9.0 Kb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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