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Series GSE100059 Query DataSets for GSE100059
Status Public on Jun 16, 2017
Title 0-12 hour transcriptional response of mouse bone marrow-derived macrophages to stimulation by LPS
Organism Mus musculus
Experiment type Expression profiling by array
Summary Innate immune response to pathogens is a complex, multi-staged process involving thousands of genes. While numerous transcription factors that act as master regulators of the response to LPS have been identified, the temporal complexity of gene expression changes strongly suggest that additional layers of regulation remain to be uncovered.
This study was done to examine response of mouse bone marrow-derived macrophages to stimulation with LPS over time. Bone marrow-derived macrophages from C57BL/6J mice were incubated in the presence of LPS for 1, 4 or 12 h, while the control group was incubated under same conditions for 4 h in the absence of the LPS. RNA isolated from these macrophages was analyzed using the Affymetrix Mouse Exon Array 1.0 ST.
 
Overall design Two sets of three female mice (strain C57BL/6J) were sacrificed at 8-12 weeks of age, and macrophages were derived from the femoral bone marrow using rhM-CSF. On day six, macrophages were divided into two treatment groups per animal, for a total of 12 samples. LPS was introduced into the medium for nine samples (one from each animal) at 10 ng/mL on day seven, and after 1, 4 or 12 h of incubation, RNA was isolated using TRIzol. Three sets of un-stimulated macrophages were harvested at 4 h and RNA isolated with TRIzol. Labeled cRNA derived from the RNA samples was hybridized to Affymetrix Mouse Exon Array 1.0 ST GeneChips. Microarray data were processed using Affymetrix power tools and brain-array CDFs (v 18.0)
 
Contributor(s) Kennedy KA, Askovich PS, Aderem A
Citation(s) 28922390
Submission date Jun 15, 2017
Last update date Jul 25, 2021
Contact name Aderem Lab
E-mail(s) alan.diercks@seattlechildrens.org
Organization name Seattle Children's Research Institute
Lab Aderem Lab
Street address 307 Westlake Avenue North, Suite 500
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platforms (1)
GPL23585 [MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [Mm_ENTREZG_v18]
Samples (12)
GSM2670686 Unstim.1
GSM2670687 LPS.4h.1
GSM2670688 Unstim.2
Relations
BioProject PRJNA390608

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Supplementary file Size Download File type/resource
GSE100059_RAW.tar 281.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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