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Series GSE10036 Query DataSets for GSE10036
Status Public on Apr 06, 2008
Title Small RNAs in four Argonaute complexes in Arabidopsis
Organism Arabidopsis thaliana
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary The core components of RNA silencing effector complexes include small RNAs and members of the Argonaute (AGO) protein family. Arabidopsis encodes ten AGOs and complex population of small RNAs. It remains largely unknown how these small RNAs are recognized and recruited by each AGO complex. Here we purified four AGO complexes and identified small RNAs in each complex by deep sequencing.
Keywords: Small RNA sorting
Overall design Small RNAs were prepared from Arabidopsis AGO1, AGO2, AGO4 and AGO5 complexes, ligated to a 3' adaptor and a 5' acceptor sequentially, and then RT-PCR amplified. PCR products were reamplified using a pair of solexa cloning primers and then provided for sequencing. For technical details, see Qi, Y., He, X., Wang, X.J., Kohany, O., Jurka, J., and Hannon, G.J. 2006. Distinct catalytic and non-catalytic roles of ARGONAUTE4 in RNA-directed DNA methylation. Nature 443(7114): 1008-1012.
Contributor(s) Mi S, Cai T, Qi Y
Citation(s) 18342361
Submission date Dec 27, 2007
Last update date Aug 10, 2016
Contact name Tao Cai
Organization name National Institute Of Biological Sciences, Beijing (NIBS)
Lab Sequencing Facility
Street address No. 7 Science Park Road, Zhongguancun Life Science Park
City Beijing
ZIP/Postal code 102206
Country China
Platforms (1)
GPL9062 Illumina Genome Analyzer (Arabidopsis thaliana)
Samples (4)
GSM253622 ATH-AGO1
GSM253623 ATH-AGO2
GSM253624 ATH-AGO4
BioProject PRJNA108599

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Supplementary file Size Download File type/resource
GSE10036_RAW.tar 256.5 Mb (http)(custom) TAR (of TXT)

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