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Series GSE100379 Query DataSets for GSE100379
Status Public on Nov 04, 2019
Title Artificial reforestation has less diverse soil nitrogen-cycling genes than natural restoration
Organisms Bacteria; Archaea; Fungi; Viruses
Experiment type Genome variation profiling by array
Summary Reforestation is effective in restoring ecosystem functions and enhancing ecosystem services of degraded land. The three most commonly employed reforestation methods of natural reforestation, artificial reforestation with native Masson pine (Pinus massoniana Lamb.), and introduced slash pine (Pinus elliottii Engelm.) plantations were equally successful in biomass yield in southern China. However, it is not known if soil ecosystem functions, such as nitrogen (N) cycling, are also successfully restored. Here, we employed a functional microarray to illustrate soil N cycling. The composition and interactions of N-cycling genes in soils varied significantly with reforestation method. Natural reforestation had more superior organization of N-cycling genes, and higher functional potential (abundance of ammonification, denitrification, assimilatory, and dissimilatory nitrate reduction to ammonium genes) in soils, providing molecular insight into the effects of reforestation.
 
Overall design The diversity and interaction of nitrogen-cycling genes in the soils of three reforestation methods were studied. The three reforestation methods were naturally restored secondary forests (NRSF), artificial reforestation with native Masson pine (Pinus massoniana Lamb.) (ARMP), and introduced slash pine (Pinus elliottii Engelm.) plantations (ARSP). Each reforestation method included 15 replicates (plots) in red soil region in southern China. After DNA extraction and outlier (ARMP1, NRSF2, and NRSF7) checked, 14, 14, and 11 microarray replicates were obtained for ARSP, ARMP, and NRSF, respectively.
 
Contributor(s) Wang Y, Zheng H, Chen F, Yang Y, Zeng J, Van Nostrand J, Zhou J, Ouyang Z
Citation Yun Wang, Hua Zheng, Falin Chen, Yunfeng Yang, Jing Zeng, Joy D. Van Nostrand, Jizhong Zhou, and Zhiyun Ouyang. Artificial reforestation produces less diverse soil nitrogen-cycling genes than natural restoration. Ecosphere 2019;10(1):e02562. doi:10.1002/ecs2.2562
Submission date Jun 22, 2017
Last update date Jul 25, 2021
Contact name Yun Wang
Organization name Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences
Street address Shuangqing Road 18th, Haidian District
City Beijing
ZIP/Postal code 100085
Country China
 
Platforms (1)
GPL23836 GeoChip 3.0 [Probe-level version]
Samples (42)
GSM2679826 Artificial restored slash pine plantations_rep1
GSM2679827 Artificial restored slash pine plantations_rep2
GSM2679828 Artificial restored slash pine plantations_rep3
Relations
BioProject PRJNA391498

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE100379_RAW.tar 155.9 Mb (http)(custom) TAR (of XLSX)
Processed data included within Sample table

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