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Series GSE100492 Query DataSets for GSE100492
Status Public on May 16, 2019
Title m6A peaks in mouse brain sample.
Organism Mus musculus
Experiment type Other
Summary RNA was isolated from 4 weeks old Prrc2a flox/flox whole brain using the TRIzol (Invitrogen) reagent by following the company manual. mRNA was isolated by using Dynabeads mRNA Purification Kit (Invitrogen) For all samples the RNA integrity was checked using an Agilent Bioanalyzer 2100. All samples showed a RIN (RNA integrity number) of higher than 9. Approximately 2.5 µg of total RNA was then used for library preparation using a TruSeq™ RNA Sample Prep Kit v2 (Illumina, San Diego, CA, USA) according to the manufacturer’s protocol.The libraries were sequenced using HiSeq3000 (Illumina) in paired-read mode, creating reads with a length of 101 bp. Sequencing chemistry v2 (Illumina) was used.
Overall design Examination of m6A peaks in 4 weeks old Prrc2a flox/flox mice.
Contributor(s) Wu R, Sun B, Li A, Yuan Z, Yang Y
Citation(s) 30514900
Submission date Jun 26, 2017
Last update date Jul 25, 2021
Contact name Bao-Fa Sun
Organization name Beijing Institute of Genomics (BIG) of Chinese Academy of Sciences (CAS)
Street address Da-Tun Road
City Beijing
ZIP/Postal code 100101
Country China
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (4)
GSM2684969 m6A IP rep1
GSM2684970 m6A IP rep2
GSM2684971 input rep1
This SubSeries is part of SuperSeries:
GSE104541 Control, Prrc2a conditional knockout, and Fto transgene mouse brain study
BioProject PRJNA391945
SRA SRP110526

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE100492_RAW.tar 810.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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