|
| Status |
Public on May 16, 2019 |
| Title |
m6A peaks in mouse brain sample. |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
RNA was isolated from 4 weeks old Prrc2a flox/flox whole brain using the TRIzol (Invitrogen) reagent by following the company manual. mRNA was isolated by using Dynabeads mRNA Purification Kit (Invitrogen) For all samples the RNA integrity was checked using an Agilent Bioanalyzer 2100. All samples showed a RIN (RNA integrity number) of higher than 9. Approximately 2.5 µg of total RNA was then used for library preparation using a TruSeq™ RNA Sample Prep Kit v2 (Illumina, San Diego, CA, USA) according to the manufacturer’s protocol.The libraries were sequenced using HiSeq3000 (Illumina) in paired-read mode, creating reads with a length of 101 bp. Sequencing chemistry v2 (Illumina) was used.
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| |
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| Overall design |
Examination of m6A peaks in 4 weeks old Prrc2a flox/flox mice.
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| |
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| Contributor(s) |
Wu R, Sun B, Li A, Yuan Z, Yang Y |
| Citation(s) |
30514900 |
| |
| Submission date |
Jun 26, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Bao-Fa Sun |
| E-mail(s) |
sunbf@big.ac.cn
|
| Organization name |
Beijing Institute of Genomics (BIG) of Chinese Academy of Sciences (CAS)
|
| Street address |
Da-Tun Road
|
| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
| |
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| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
|
| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE104541 |
Control, Prrc2a conditional knockout, and Fto transgene mouse brain study |
|
| Relations |
| BioProject |
PRJNA391945 |
| SRA |
SRP110526 |
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