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Series GSE100528 Query DataSets for GSE100528
Status Public on Jul 15, 2018
Title METTL3-mediated m6A modification is required for cerebellar development m6A modification and cerebellar development
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary N6-methyladenosine (m6A) RNA methylation is the most abundant modification on mRNAs and plays important roles in various biological processes. The formation of m6A is catalyzed by a methyltransferase complex including methyltransferase like 3 (METTL3) as a key factor. However, the in vivo functions of METTL3 and m6A modification in mammalian development remain unclear. Here we show that specific inactivation of Mettl3 in mouse nervous system causes severe developmental defects in the brain. Mettl3 conditional knockout mice manifest cerebellar hypoplasia caused by drastically enhanced apoptosis of new born cerebellar granule cells (CGCs) in the external granular layer (EGL). METTL3 depletion induced loss of m6A modification causes extended RNA half-lives and aberrant splicing events, consequently leading to dysregulation of transcriptome-wide gene expression and premature CGC death. Our findings reveal a critical role of METTL3-mediated m6A in regulating the development of mammalian cerebellum.
Overall design The mRNA expression and m6A modification were analyzed in wildtype and Mettl3 conditional knockout (Nestin-Cre) mice using postnatal day 7 and day 14 mouse cerebellums.
Contributor(s) Wang C, Cui G, Liu X, Xu K, Wang M, Zhang X, Jiang L, Li A, Yang Y, Lai W, Sun B, Jiang G, Wang H, Tong W, Li W, Wang X, Yang Y, Zhou Q
Citation(s) 29879109
Submission date Jun 27, 2017
Last update date Jul 25, 2021
Contact name Xiuying Liu
Organization name Changping Laboratory
Street address Yard 28, Science Park Road, Changping District
City Beijing
ZIP/Postal code 102206
Country China
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (16)
GSM2686019 P7RNA_WT1
GSM2686020 P7RNA_WT2
GSM2686021 P7RNA_cKO1
BioProject PRJNA392036
SRA SRP110576

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Supplementary file Size Download File type/resource
GSE100528_RAW.tar 1.6 Gb (http)(custom) TAR (of BW)
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Raw data are available in SRA
Processed data provided as supplementary file

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