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Series GSE100559 Query DataSets for GSE100559
Status Public on Jan 25, 2018
Title Cross-linking and immunoprecipitation (CLIP) analysis for identifying binding sites of Tet2 in bone marrow derived mast cell
Organism Mus musculus
Experiment type Other
Summary To transcriptome-widely identify the mRNA targets of Tet2 and investigate the immunological function of Tet2 at RNA regulation level in vivo, we performed CLIP-seq of Tet2 in BMMCs. We performed three bio-replicates of CLIP-seq for getting potential Tet2-binding mRNAs.Using data of normalized tag numbers in the common peaks, we found that biological replicates correlated well with each other.More than 60% of the peaks located in genic regions, which were preferentially enriched in coding sequencing (CDS) among mature mRNA elements.
Overall design Systematically identify Tet2-binding RNAs in biological triplicate.
Contributor(s) Shen Q, Cao X
Citation(s) 29364877
Submission date Jun 27, 2017
Last update date May 15, 2019
Contact name Qian Zhang
Phone +8615921196443
Organization name Second military medical university
Street address xiangyin road 800
City shang hai
ZIP/Postal code 200433
Country China
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (3)
GSM2686818 Tet2 rep1 (CLIP-Seq)
GSM2686819 Tet2 rep2 (CLIP-Seq)
GSM2686820 Tet2 rep3 (CLIP-Seq)
BioProject PRJNA392110
SRA SRP110607

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE100559_peaks.txt.gz 80.5 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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