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Series GSE101343 Query DataSets for GSE101343
Status Public on Jul 18, 2017
Title RNA sequencing to compare gene expession in hepatic stellate cells on a soft (400 Pa) and stiff (25000 Pa) polyacrylamide hydrogels
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Primary human hepatic stellate cells (HSCs) isolated from healthy patients were purchased from Sciencell. They were seeded on either 400 Pa or 25000 Pa polyacrylamide hydrogels for 2 days and collected for RNA isolation. The goal of this study is to determine genes transcriptionally regulated by a stiff matrix. 2 RNA samples of HSCs on 400 Pa and 3 RNA samples of HSCs on 25600 Pa were sent to the University of Minnesota Genomics Center for RNA sequencing.
Overall design 2 RNA samples isolated from HSC on 400 Pa stiffness and 3 from HSCs on 25600 Pa stiffness. They were converted to Illumina sequencing libraries using Illumina’s Truseq Stranded mRNA Sample Preparation Kit. Truseq libraries were then subjected to cluster using Illumina cBot instrument and sequencing using HiSeq2500
Contributor(s) Kang N, Dou C
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Submission date Jul 12, 2017
Last update date May 15, 2019
Contact name Ningling Kang
Organization name University of Minnesota
Department Hormel Institute
Street address 801 16th AVE NE
City Austin
State/province MN
ZIP/Postal code 55912
Country USA
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (5)
GSM2701558 25600-1
GSM2701559 25600-2
GSM2701560 25600-3
BioProject PRJNA394015
SRA SRP111737

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE101343_genes.fpkm_table.xlsx 1.4 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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