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Status |
Public on Feb 13, 2019 |
Title |
Global fine-tuning of translation by queuosinylation and Dnmt2-dependent tRNA methylation |
Organism |
Schizosaccharomyces pombe |
Experiment type |
Expression profiling by high throughput sequencing Non-coding RNA profiling by high throughput sequencing
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Summary |
In most eukaryotes, the wobble position of tRNA with a GUN anticodon is queuosine-modified (Q34). Q is synthesized exclusively by eubacteria and salvaged by eukaryotes as a nutrient to replace G34 in tRNAs. Q34 modification stimulates Dnmt2/Pmt1-dependent C38 methylation in the tRNAAsp anticodon loop in Schizosaccharomyces pombe. Due to the location of both modification in the anticodon loop, we anticipated an influence on translation. Our experimental setup allowed for the analysis of effects from each modification alone or a combination of both.
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Overall design |
Cytoplasmic RNA and Cytoplasmic RNA protected by ribosome from RNaseI cleavage, were sequenced. Small RNA libraries were performed according to the protocol using NEB NEXT Small RNA library Prep Set for Illumina (Multiplex Compatible) E7330, and size-selected (120-170 base pairs) before sequencing.
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Contributor(s) |
Müller M, Legrand C |
Citation(s) |
30715423 |
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Submission date |
Aug 08, 2017 |
Last update date |
May 15, 2019 |
Contact name |
Ann E. Ehrenhofer-Murray |
Organization name |
Humboldt-Universität zu Berlin
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Department |
Institut für Biologie
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Street address |
Philippstr. 13
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City |
Berlin |
ZIP/Postal code |
10099 |
Country |
Germany |
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Platforms (2) |
GPL13988 |
Illumina HiSeq 2000 (Schizosaccharomyces pombe) |
GPL20584 |
Illumina NextSeq 500 (Schizosaccharomyces pombe) |
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Samples (36)
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Relations |
BioProject |
PRJNA397588 |
SRA |
SRP115046 |