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Series GSE10238 Query DataSets for GSE10238
Status Public on Mar 31, 2008
Title Complement activation in the peripheral nervous system following the spinal nerve ligation model of neuropathic pain
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary Neuroinflammatory and neuroimmune mechanisms, as exemplified by infiltrating immune cells and activation of resident endothelial/glial cells, respectively, are known to be involved in the establishment and maintenance of chronic pain. An immune system pathway that may be involved in the activation of both immune and glial cells is complement. The complement pathway is made up of a large number of distinct plasma proteins which react with one another to opsonize pathogens and induce a series of inflammatory responses to help fight infection. Cleaved products and complexes produced by complement activation are responsible for a range of effects including mediation of immune infiltration, activation of phagocytes, opsonization/lysis of pathogens and injured cells, and production of vasoactive amines such as histamine and serotonin.
Gene-expression microarray-analysis was performed on the rat spinal nerve ligation (SNL) model of neuropathic pain
Keywords: organ type comparison, disease state analysis and drug treatment
Overall design For gene expression analysis, rats from five experimental groups were sacrificed 19–21 days after surgery: (1)Naïve+ vehicle; (2) Naïve + GPN; (3) sham + vehicle; (4) SNL + vehicle; (5) SNL + GPN. Tissue was collected from whole brain, hemisected spinal cord, mid thigh sciatic nerve, and L4, L5 and L6 dorsal root ganglia (DRG), both ipsilateral(ipsi) and contralateral (contra) to the injury. In addition,‘‘organ recital’’ tissues were harvested from naı ¨ve animals: adrenal,aorta, fetal brain, kidney, liver, quadriceps muscle, spleen,submaxillary gland, and testis and 15 other tissues.For each experimental group and tissue, samples rapidly frozen on dry ice were separated into two pools(Pool 1 and Pool 2), consisting of half or 4-6 animals each.Pool 1 total RNA was subjected to standard methods on Affymetrix GeneChip Arrays using rat U34 A, B, and C.
Contributor(s) Levin ME, Jin JG, Ji R, Tong J, Pomonis JD, Lavery DJ, Miller SW, Chiang LW
Citation(s) 18160218
Submission date Jan 22, 2008
Last update date Feb 21, 2017
Contact name Jason Gang Jin
Phone 732-220-9988
Fax 732-960-2355
Organization name MaxyBio
Street address 675 US Highway
City North Brunswick
ZIP/Postal code 08902
Country USA
Platforms (3)
GPL85 [RG_U34A] Affymetrix Rat Genome U34 Array
GPL86 [RG_U34B] Affymetrix Rat Genome U34 Array
GPL87 [RG_U34C] Affymetrix Rat Genome U34 Array
Samples (156)
GSM258319 Naive L4 DRG,Ipsi set A
GSM258320 Naive L5 DRG,Ipsi set A
GSM258321 Naive L6 DRG,Ipsi set A
BioProject PRJNA108415

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Supplementary file Size Download File type/resource
GSE10238_RAW.tar 283.8 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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