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Series GSE103574 Query DataSets for GSE103574
Status Public on Nov 16, 2017
Title YY1 is a structural regulator of enhancer-promoter loops [scRNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary There is considerable evidence that chromosome structure plays important roles in gene control, but we have limited understanding of the proteins that contribute to structural interactions between gene promoters and their enhancer elements. Large DNA loops that encompass genes and their regulatory elements depend on CTCF-CTCF interactions, but most enhancer-promoter interactions do not depend on this structural protein. Here we show that the transcription factor Yin Yang 1 (YY1) contributes to enhancer-promoter structural interactions in a manner analogous to DNA interactions mediated by CTCF. YY1 binds to active enhancers and promoter-proximal elements in all cells examined. YY1 forms dimers that can facilitate DNA interactions. Deletion of YY1 binding sites or depletion of YY1 can disrupt enhancer-promoter looping and normal gene expression. We propose that YY1-mediated enhancer-promoter interactions are a general feature of mammalian gene control.
 
Overall design Single-cell RNA-seq in mouse embryonic stem cells with and without YY1 protein
 
Contributor(s) Weintraub AS, Li CH, Zamudio A, Sigova AA, Hannett NM, Day DS, Abraham BJ, Cohen M, Nabet B, Buckley DL, Guo YE, Hnisz D, Jaenisch R, Bradner JE, Gray NS, Young RA
Citation(s) 29224777
Submission date Sep 06, 2017
Last update date Jul 25, 2021
Contact name Richard A Young
E-mail(s) young_computation@wi.mit.edu
Phone 617-258-5219
Organization name Whitehead Institute for Biomedical Research
Lab Young Lab
Street address 9 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (2)
GSM2774584 scRNAseq_EB_noDrug
GSM2774585 scRNAseq_EB_yesDrug
This SubSeries is part of SuperSeries:
GSE99521 YY1 is a structural regulator of enhancer-promoter loops
Relations
BioProject PRJNA401930
SRA SRP116928

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Supplementary file Size Download File type/resource
GSE103574_RAW.tar 113.5 Mb (http)(custom) TAR (of CLOUPE, CSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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