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Series GSE104022 Query DataSets for GSE104022
Status Public on Mar 01, 2018
Title Molecular consequences of Dlx3 deletion by tamoxifen inducible K14 Cre in adult mouse epidermis (P42-P46) as determined by RNASeq analysis
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary DLX3 is a homeodomain transcription factor involved in epidermal and hair follicle differentiation. Epidermal specific deletion of DLX3 leads to IL17 related inflammation.
However, the downstream signals from DLX3-deficient keratinocytes that initiates IL17 associated inflammation is not well-characterized. Therefore we performed acute Dlx3 deletion to identify the initial molecular changes causing the skin inflammatory reponse and tried to rescue the immune phenotype by STAT3 inhibitor treatment (Cryptotanshinone).
In this study, we compared the epidermal and dermal transcriptome from Dlx3 wild-type (WT) and K14CreERT;DLX3 fl/fl (icKO).
Overall design Total RNA was extracted epidermis and dermis of WT and icKO mice. cDNA libraries were generated using NEBnext and NuGen kits. Sequencing was performed on the HiSeq 2500 and HiSeq 3000.
Contributor(s) Bhattacharya S, Kim J, Morasso M
Citation(s) 29246798
Submission date Sep 19, 2017
Last update date May 15, 2019
Contact name Maria Irene Morasso
Phone 3014357842
Organization name NIAMS NIH
Lab Laboratory of Skin Biology
Street address Bldg 50
City Bethesda
State/province Maryland
ZIP/Postal code 20892
Country USA
Platforms (2)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (29)
GSM2787888 WT_3day_Epi_1
GSM2787889 WT_3day_Epi_2
GSM2787890 WT_3day_Epi_3
BioProject PRJNA407977
SRA SRP118141

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Supplementary file Size Download File type/resource
GSE104022_150312_0221m_ANOVA_derm_ip_1week_KOvsWT.xlsx 3.6 Mb (ftp)(http) XLSX
GSE104022_150312_0221m_ANOVA_epi_topical_KOvsWT.xlsx 3.5 Mb (ftp)(http) XLSX
GSE104022_150312_0221m_RNA-Seq_results.transcript.rpkm.xlsx 4.2 Mb (ftp)(http) XLSX
GSE104022_170113_RNA-Seq_results.transcript.rpkm.xlsx 3.7 Mb (ftp)(http) XLSX
GSE104022_170113_RNA-Seq_transcript_ANOVA_DLX3_DERM_KOvsWT.xlsx 3.7 Mb (ftp)(http) XLSX
GSE104022_170703_dlx3_ANOVA.xlsx 5.0 Mb (ftp)(http) XLSX
GSE104022_170703_dlx3_RNA-Seq_results.gene.rpkm.xlsx 3.8 Mb (ftp)(http) XLSX
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