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GEO help: Mouse over screen elements for information. |
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Status |
Public on May 01, 2018 |
Title |
Expression profiling from mouse embryonic stem cells (ESCs) |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
Sin3a, a known master scaffold, provides unique contact surfaces for interaction with particular accessory proteins to repress the transcription of specific genes. Surprisingly, our results also suggest that Sin3a has a role in transcriptional activation. We compared gene expression differences between Sin3a knockdown and control ESCs with mouse gene expression microarrays and identified an approximately equal distribution of up- and down-regulated genes following Sin3a knockdown in mouse ESCs. We propose that Sin3a collaborates with Tet1 to demethylate adjacent genomic regions and, ultimately, facilitates actively transcribed gene expression in mouse ESCs.
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Overall design |
Control (shCtrl) or Sin3a knockdown (shSin3a-1) mouse ESCs were used.
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Contributor(s) |
Zhu F, Zhu Q, Kang J |
Citation(s) |
29733394 |
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Submission date |
Sep 25, 2017 |
Last update date |
Jul 25, 2021 |
Contact name |
Qianshu Zhu |
E-mail(s) |
zhuqianshu@tongji.edu.cn
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Organization name |
Tongji University
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Department |
School of Life Science and Technology
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Street address |
Siping Road
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City |
Shanghai |
State/province |
Shanghai |
ZIP/Postal code |
270000 |
Country |
China |
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Platforms (1) |
GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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Samples (2) |
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Relations |
BioProject |
PRJNA412002 |
Supplementary file |
Size |
Download |
File type/resource |
GSE104192_RAW.tar |
8.1 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
Processed data included within Sample table |
Processed data provided as supplementary file |
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