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| Status |
Public on Feb 08, 2008 |
| Title |
Genetic Analysis of opaque 2 Modifier Loci in Quality Protein Maize |
| Organism |
Zea mays |
| Experiment type |
Expression profiling by array
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| Summary |
Quality Protein Maize (QPM) was created by selecting for genetic modifiers that convert the starchy endosperm of an opaque2 (o2) mutant to a hard, vitreous phenotype. Genetic analysis has shown there are multiple, unlinked o2 modifiers (Opm), but their identity and mode of action are unknown. A microarray hybridization performed with RNA obtained from true breeding o2 progeny with vitreous and opaque kernel phenotypes identified a small group of differentially expressed genes, some of which map at or near the Opm QTLs.
Keywords: Genetic modification
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| Overall design |
Compared 18 days after pollination endosperm transcript profiles in true breeding modified (vitreous; V) and non-modified (opaque; O) opaque 2 kernels. Used four vitreous biological reps (V1-V4) and four opaque biological reps (O1-O4). Each biological rep is a pool of 25 kernels representing 5 kernels form each of 5 uniform phenotype ears (vitreous or opaque). Four arrays used: Array 1= V1 vs O1, Array 2 = V2 vs O2, Array 3 = V3 vs O3, Array 4 = V4 vs O4
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| Contributor(s) |
Holding DR, Larkins BA |
| Citation(s) |
18427771 |
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| Submission date |
Feb 08, 2008 |
| Last update date |
Mar 19, 2012 |
| Contact name |
David Richard Holding |
| E-mail(s) |
dholding@ag.arizona.edu
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| Phone |
520 621 9154
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| Organization name |
University of Arizona
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| Department |
Plant Sciences
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| Lab |
Brian Larkins
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| Street address |
303 Forbes Building
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| City |
Tucson |
| State/province |
AZ |
| ZIP/Postal code |
85721 |
| Country |
USA |
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| Platforms (1) |
| GPL6438 |
Maize oligonucleotide array 46K version |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA108001 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE10449_RAW.tar |
25.2 Mb |
(http)(custom) |
TAR (of GPR) |
| Processed data included within Sample table |
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