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Series GSE105117 Query DataSets for GSE105117
Status Public on Jul 21, 2019
Title 1,25(OH)D3 effect on colonic organoids from wt and VDR KO mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary VitaminD deficiency has been related to a higher incidence of colorectal cancer. In order to further study the effect of VDR and its ligand as genome modulators we established stem cell enriched cultures of wt and VDR KO mice, treated with the active metabolite of Vitamin D and analyzed the changes in total RNA expression.
Overall design Organoid 3D stem cell enriched culture was established from colon crypts from 3 wt mice and 3 VDR KO mice. The cultures were treated for 72 houres with either vehicle (EtOH) or VDR ligand 1,25(OH)2D3, the active form of VitaminD. Gene expression was analyzed with an Agilent microarray design ID 028005, P/N G4852A.
Contributor(s) Barbáchano A, Fernández-Barral A, Costales-Carrera A, Ferrer-Mayorga G, Larriba MJ, Muñoz A, Fernández-Barral A
Citation(s) 31306552
Submission date Oct 18, 2017
Last update date Jul 25, 2021
Contact name Alba Costales
Phone 0034647906865
Organization name IIB
Department cancer genetics
Lab 231
Street address Arturo Duperier, 4
City madrid
State/province madrid
ZIP/Postal code 28029
Country Spain
Platforms (1)
GPL13912 Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Feature Number version)
Samples (12)
GSM2818530 wt 1 control
GSM2818531 wt 1 tratamiento
GSM2818532 ko 1 control
BioProject PRJNA414728

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE105117_RAW.tar 46.1 Mb (http)(custom) TAR (of TXT)
GSE105117_ko_tto-ko_control.xlsx.gz 16.0 Mb (ftp)(http) XLSX
GSE105117_ko_tto-wt_tto.xlsx.gz 16.0 Mb (ftp)(http) XLSX
GSE105117_wt_tto-wt_control.xlsx.gz 16.1 Mb (ftp)(http) XLSX
Processed data included within Sample table
Processed data are available on Series record

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