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Status |
Public on Nov 20, 2018 |
Title |
A metabolic checkpoint controls hyphal development in Candida albicans via nitric oxide signaling |
Organism |
Candida albicans |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We investigated the roles of mitochondrial dynamics in hyphal growth of C. albicans using the small molecule inhibitor MDIVI-1. Strikingly, the small molecule inhibitor represses both the yeast-to hyphae transition and ongoing filamentation, and its effects on morphogenesis can be uncoupled from general growth inhibition. RNAseq experiments of inhibitor-treated cells coupled with Candida mutant analyses suggest the existence of a novel mechanism of action to represses hyphal growth. The inhibitor was protective to host cells, increasing the survival of bone-marrow derived macrophages in ex vivo macrophage-Candida infection assays, suggesting it has potential as a therapeutic.
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Overall design |
Analysis of RNA-seq in C. albicans
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Contributor(s) |
Traven A, Harrrison PF, Beilharz TH |
Citation(s) |
30463019 |
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Submission date |
Oct 19, 2017 |
Last update date |
Sep 08, 2020 |
Contact name |
Traude Beilharz |
E-mail(s) |
traude.beilharz@monash.edu
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Organization name |
Monash University
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Department |
Biomedicine Discovery Institute
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Lab |
RNA Systems Biology Laboratory
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Street address |
Wellington Rd
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City |
Clayton |
State/province |
VIC |
ZIP/Postal code |
3800 |
Country |
Australia |
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Platforms (1) |
GPL23573 |
Illumina HiSeq 1500 (Candida albicans) |
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Samples (28)
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Relations |
BioProject |
PRJNA414964 |
SRA |
SRP120479 |