|
| Status |
Public on Nov 20, 2018 |
| Title |
A metabolic checkpoint controls hyphal development in Candida albicans via nitric oxide signaling |
| Organism |
Candida albicans |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We investigated the roles of mitochondrial dynamics in hyphal growth of C. albicans using the small molecule inhibitor MDIVI-1. Strikingly, the small molecule inhibitor represses both the yeast-to hyphae transition and ongoing filamentation, and its effects on morphogenesis can be uncoupled from general growth inhibition. RNAseq experiments of inhibitor-treated cells coupled with Candida mutant analyses suggest the existence of a novel mechanism of action to represses hyphal growth. The inhibitor was protective to host cells, increasing the survival of bone-marrow derived macrophages in ex vivo macrophage-Candida infection assays, suggesting it has potential as a therapeutic.
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| |
|
| Overall design |
Analysis of RNA-seq in C. albicans
|
| |
|
| Contributor(s) |
Traven A, Harrrison PF, Beilharz TH |
| Citation(s) |
30463019 |
| |
| Submission date |
Oct 19, 2017 |
| Last update date |
Sep 08, 2020 |
| Contact name |
Traude Beilharz |
| E-mail(s) |
traude.beilharz@monash.edu
|
| Organization name |
Monash University
|
| Department |
Biomedicine Discovery Institute
|
| Lab |
RNA Systems Biology Laboratory
|
| Street address |
Wellington Rd
|
| City |
Clayton |
| State/province |
VIC |
| ZIP/Postal code |
3800 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL23573 |
Illumina HiSeq 1500 (Candida albicans) |
|
| Samples (28)
|
|
| Relations |
| BioProject |
PRJNA414964 |
| SRA |
SRP120479 |
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