|
| Status |
Public on Dec 22, 2008 |
| Title |
AML1/ETO binding pattern on human promoters |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
Approximately 20% of Acute Myelogenous Leukemia (AML) cases carry the t(8;21) translocation, which involves the AML1 and ETO genes, and express the resulting AML1/ETO fusion protein that functions as a transcriptional repressor by recruiting NCoR/SMRT/HDAC complexes to DNA.
We used microarrays to identify human promoters bound by AML1/ETO in U937 cells.
Keywords: ChIP-chip
|
| |
|
| Overall design |
A U937 cell line that conditionally expresses HA-tagged AML1/ETO under the control of the mouse metallothionine promoter (U937-A1E) (Alcalay et al., J.Clin.Invest, 2003,112, 1751-1761) was used. Cells were treated for 8h with 100uM ZnSO4 to induce transgene expression, and ChIP was performed using an anti-HA antibody. ChIP products were then PCR amplified, labeled with Cy3/Cy5 fluorescent dyes and hybridized to the NimbleGen HG17 Human Promoter 2 Array set, which explores 4 kb upstream and 1 kb downstream the transcription start site (TSS) of 24,434 annotated genes. Two biological replicates were prepared and hybridized to independent array sets. U937-Mt cells, which carry the empty vector, served as control for non-specific binding of the anti-HA antibody.
|
| |
|
| Contributor(s) |
Alcalay M |
| Citation(s) |
19043539 |
| |
| Submission date |
Feb 14, 2008 |
| Last update date |
Mar 19, 2012 |
| Contact name |
Myriam Alcalay |
| E-mail(s) |
myriam.alcalay@ieo.eu
|
| Organization name |
European Institute of Oncology
|
| Department |
Experimental Oncology
|
| Lab |
Functional Genomics
|
| Street address |
Via Adamello 16
|
| City |
Milan |
| ZIP/Postal code |
20139 |
| Country |
Italy |
| |
|
| Platforms (2) |
| GPL6492 |
NimbleGen Systems Human HG17 Promoter 386K Array1 version 1 |
| GPL6493 |
NimbleGen Systems Human HG17 Promoter 367K Array2 version 1 |
|
| Samples (8)
|
| GSM266016 |
U937 cells expressing AML1/ETO-HA_ChIP with anti-HA antibody_replica1_array1 |
| GSM266017 |
U937 cells expressing AML1/ETO-HA_ChIP with anti-HA antibody_replica2_array1 |
| GSM266018 |
U937 cells transfected with empty pSGMtNeo vector_ChIP with anti-HA antibody_replica1_array1 |
| GSM266019 |
U937 cells transfected with empty pSGMtNeo vector_ChIP with anti-HA antibody_replica2_array1 |
| GSM266030 |
U937 cells expressing AML1/ETO-HA_ChIP with anti-HA antibody_replica1_array2 |
| GSM266031 |
U937 cells expressing AML1/ETO-HA_ChIP with anti-HA antibody_replica2_array2 |
| GSM266032 |
U937 cells transfected with empty pSGMtNeo vector_ChIP with anti-HA antibody_replica1_array2 |
| GSM266033 |
U937 cells transfected with empty pSGMtNeo vector_ChIP with anti-HA antibody_replica2_array2 |
|
| This SubSeries is part of SuperSeries: |
| GSE10537 |
Gene expression profile and DNA binding pattern of AML1/ETO in U937 cells |
|
| Relations |
| BioProject |
PRJNA108945 |
Less...
More...