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| Status |
Public on Nov 28, 2008 |
| Title |
Analysis of expression levels of genes on chromosome 19 in U937 cells expressing AML1/ETO |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by genome tiling array
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| Summary |
Approximately 20% of Acute Myelogenous Leukemia (AML) cases carry the t(8;21) translocation, which involves the AML1 and ETO genes, and express the resulting AML1/ETO fusion protein that functions as a transcriptional repressor by recruiting NCoR/SMRT/HDAC complexes to DNA.
We used ChIP-chip to identify the determinants of AML1/ETO binding on a contiguous DNA region (chromosome 19). In order to correlate transcription factor binding to gene expression, we evaluated expression levels of genes localized on chromosome 19 by expression tiling.
Keywords: Gene expression
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| Overall design |
A U937 cell line that conditionally expresses HA-tagged AML1/ETO under the control of the mouse metallothionine promoter (U937-A1E) (Alcalay et al., J.Clin.Invest, 2003,112, 1751-1761) was used. A cell line carrying the empty vector was used as control. Cell lines were treated for 8h with 100uM ZnSO4 to induce transgene expression in U937-A1E. For each of the U937 cell lines (AML1/ETO and Mt), three independent RNA extractions were performed, and an equal quantity of each of the three RNA preparations was then mixed to generate an RNA pool for each sample.
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| Contributor(s) |
Alcalay M |
| Citation(s) |
19043539 |
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| Submission date |
Feb 20, 2008 |
| Last update date |
Mar 19, 2012 |
| Contact name |
Myriam Alcalay |
| E-mail(s) |
myriam.alcalay@ieo.eu
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| Organization name |
European Institute of Oncology
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| Department |
Experimental Oncology
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| Lab |
Functional Genomics
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| Street address |
Via Adamello 16
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| City |
Milan |
| ZIP/Postal code |
20139 |
| Country |
Italy |
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| Platforms (1) |
| GPL6510 |
NimbleGen Systems Human HG17 Chromosome19 386K Array1 version1 |
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| Samples (4)
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| GSM266906 |
U937 cells expressing AML1/ETO - Replica 1 |
| GSM266907 |
U937 cells expressing AML1/ETO - Replica 2 |
| GSM266908 |
U937 cells transfected with empty pSGMtNeo vector - Replica 1 |
| GSM266909 |
U937 cells transfected with empty pSGMtNeo vector - Replica 2 |
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| This SubSeries is part of SuperSeries: |
| GSE10537 |
Gene expression profile and DNA binding pattern of AML1/ETO in U937 cells |
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| Relations |
| BioProject |
PRJNA108839 |
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