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Series GSE10585 Query DataSets for GSE10585
Status Public on Feb 09, 2009
Title Egr1 regulates the coordinated expression of numerous EGF receptor target genes as identified by ChIP-on-chip
Organism Homo sapiens
Experiment type Expression profiling by array
Genome binding/occupancy profiling by genome tiling array
Summary Previous studies have revealed that UV-stimulation of a variety of cells leads to activation of the EGF receptor, induction of Egr1, growth inhibition and apoptosis. On the other hand both Egr1 and EGF receptor activation are implicated in promoting the progression of prostate cancer. We treated M12 tumorigenic prostate epithelial cells which express little Egr1 with UV irradiation which rapidly activated the EGF receptor and elevated Egr1. Treatment with specific EGFR and ERKI/II inhibitors (PD153035 and UO126, respectively) confirmed that the upregulation of Egr1 was downstream of EGFR and ERKI/II Map kinase pathway. ChIP on chip experiments using Egr1 antibody identified 288 significantly bound promoters upon UV stimulation. Of these target genes, 40% had consensus Egr1 site in their promoters, considerably greater than that expected by chance (p < 0.005). The array binding results were validated by PCR analysis of 25 genes using DNA from conventional IP experiment. Affymetrix gene expression analysis of UV treated and control cells confirmed that a significant number of these bound promoters showed gene expression changes. Addition of siRNA to Egr1 confirmed that the gene expression changes were dependent upon Egr1 expression. Addition of EGF led to similar expression changes for nine tested genes. Proliferation and apoptosis assays confirmed that M12 cells undergo growth arrest and apoptosis following UV irradiation. Moreover, addition of EGF also promoted significant growth inhibition. These results indicate the M12 cells undergo a EGF receptor dependent apoptosis response upon UV-stimulation and that Egr1 mediates the regulation of numerous genes downstream of the EGF receptor that are associated with this response.
Keywords: UV treatment analysis
Overall design duplicated experiment for Affymetrix gene expression analysis and Chip-on-Chip analysis.
Contributor(s) Arora S, Wang Y, McClelland M, Mercola D
Citation(s) 19032775
Submission date Feb 20, 2008
Last update date Mar 25, 2019
Contact name Yipeng Wang
Phone 8585813960
Organization name Vaccine Research Institute of San Diego
Street address
City San Diego
State/province CA
ZIP/Postal code 92121
Country USA
Platforms (3)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
GPL6441 Sidney Kimmel Cancer Center Human Promotor 19K Array, v13, set-A
GPL6442 Sidney Kimmel Cancer Center Human Promotor 19K Array, v13, set-B
Samples (8)
GSM265400 Egr1 Chip-on-Chip, M12 untreated va. UV treated, duplicate 1, SetA Array
GSM265401 Egr1 Chip-on-Chip, M12 untreated va. UV treated, duplicate 1, SetB Array
GSM265402 Egr1 Chip-on-Chip, M12 untreated vs. UV treated, duplicate 2, SetA Array
BioProject PRJNA107853

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE10585_RAW.tar 28.7 Mb (http)(custom) TAR (of CEL, TXT)
Processed data included within Sample table

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