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Series GSE106115 Query DataSets for GSE106115
Status Public on Dec 31, 2017
Title microRNA-triggered transposon small RNAs mediate genome dosage response (mir845 sRNA-Seq)
Organism Arabidopsis thaliana
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Chromosome dosage plays a significant role in reproductive isolation and speciation in both plants and animals, but underlying mechanisms are largely obscure. Transposable elements can promote hybridity through maternal small RNA, and have been postulated to regulate dosage response via neighboring imprinted genes. Here, we show that a highly conserved microRNA in plants, miR845, targets the tRNAMet primer-binding site (PBS) of LTR-retrotransposons in Arabidopsis pollen, and triggers the accumulation of 21 to 22-nucleotide small RNA in a dose dependent fashion via RNA polymerase IV. We show that these epigenetically activated small-interfering RNAs (easiRNAs) mediate hybridization barriers between diploid seed parents and tetraploid pollen parents (“the triploid block”), and that natural variation for miR845 may account for “endosperm balance” allowing formation of triploid seeds. Targeting the PBS with small RNA is a common mechanism for transposon control in mammals and plants, and provides a uniquely sensitive means to monitor chromosome dosage and imprinting in the developing seed.
 
Overall design Small RNA sequencing of Arabidopsis pollen
 
Contributor(s) Borges F, Köhler C
Citation(s) 29335544
Submission date Oct 24, 2017
Last update date May 15, 2019
Contact name Filipe Borges
E-mail(s) filipe.borges@inrae.fr
Organization name Institut Jean-Pierre Bourgin - INRAE
Street address Route de St-Cyr (RD10)
City Versailles
ZIP/Postal code 78026
Country France
 
Platforms (1)
GPL17639 Illumina HiSeq 2500 (Arabidopsis thaliana)
Samples (4)
GSM2829922 mir845 1n rep1
GSM2829923 mir845 1n rep2
GSM2829924 mir845 2n rep1
This SubSeries is part of SuperSeries:
GSE106117 microRNA-triggered transposon small RNAs mediate genome dosage response
Relations
BioProject PRJNA415635
SRA SRP121335

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE106115_RAW.tar 60.3 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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