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Status |
Public on Apr 25, 2018 |
Title |
Transcriptome analyses of three Zymoseptoria tritici isolates during infection of Triticum aestivum |
Organisms |
Triticum aestivum; Zymoseptoria tritici |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We collected infected wheat leaf material at up to nine time points per Z. tritici isolate and conducted confocal microscopy analyses to select samples for RNA extraction and transcriptome sequencing based on the morphological infection stage. Thereby, we generated stage-specific RNA-seq datasets corresponding to the four core infection stages allowing us to compare the isolate-specific expression profiles at the same developmental stage of infection. Our final dataset comprises four stage-specific transcriptomes per isolate with two biological replicates per sample. Comparative transcriptome analyses reveal that the expression phenotypes of the three isolates differ significantly.
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Overall design |
Comparative analyses of stage-specific and isolate-specific expression profiles of three Z. tritici isolates
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Contributor(s) |
Haueisen J, Stukenbrock EH |
Citation(s) |
30680113, 32842972, 33724368, 35108092 |
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Submission date |
Oct 25, 2017 |
Last update date |
Mar 31, 2022 |
Contact name |
Janine Haueisen |
E-mail(s) |
janine.haueisen@gmail.com
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Organization name |
Christian-Albrechts University of Kiel
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Department |
Botanical Institute
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Lab |
Environmental Genomics
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Street address |
Am Botanischen Garten 1-9
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City |
Kiel |
ZIP/Postal code |
24118 |
Country |
Germany |
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Platforms (1) |
GPL24181 |
Illumina HiSeq 2500 (Triticum aestivum; Zymoseptoria tritici) |
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Samples (24)
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Relations |
BioProject |
PRJNA415716 |
SRA |
SRP121407 |