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Series GSE106204 Query DataSets for GSE106204
Status Public on Jul 08, 2018
Title RNA-seq in WT and H3.3 G34R mouse ES cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Sequencing of paediatric gliomas has identified two common substitution mutations (K27M and G34R) in genes encoding histone H3.3. We introduced a single-copy H3.3 G34R targeted mutation in mouse ES cells and observed gains in H3K36me3 and H3K9me3 across the genome. Altered chromatin profiles correlated with enrichment of KDM4 A/B/C, a histone lysine (K9/K36) demethylase. RNA-seq of H3.3 G34R mutant showed disrupted gene expression patterns which also correlated with KDM4 enrichment. Expression of a single copy of H3.3 G34R at endogenous levels was sufficient to genocopy KDM4 triple-KO cells as determined by ChIP-seq and RNA-seq.
Overall design 2 samples - RNA-seq of WT control and H3.3 G34R mutant cells
Contributor(s) Voon HP, Wong LH
Citation(s) 30087349
Submission date Oct 26, 2017
Last update date May 15, 2019
Contact name Hsiao Phin Joanna Voon
Organization name Monash University
Department Biochemistry and Molecular Biology
Lab Epigenetics and Chromatin
Street address Monash University, Wellington Road
City Clayton
State/province Victoria
ZIP/Postal code 3800
Country Australia
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (2)
GSM2831543 WT129_RNASeq
GSM2831544 G34R_RNASeq
This SubSeries is part of SuperSeries:
GSE106205 The H3.3 G34R glioma-associated mutation alters H3K9me3 and H3K36me3 chromatin modifications
BioProject PRJNA415917
SRA SRP121628

Download family Format
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Supplementary file Size Download File type/resource
GSE106204_RAW.tar 142.3 Mb (http)(custom) TAR (of BW, TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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