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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jan 17, 2018 |
| Title |
single-cell-RNA-sequencing analysis of naive and Listeria monocytogenes-specific CD8+ T cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The histone methyltransferase Suv39h1 silences transcriptional programs during CD8+-T cell differentiation
The study explores the role of gene silencing by Suv39h1, a histone methyltransferase, that plays a critical role in heterochromatin dynamics.
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| Overall design |
Naïve littermate and Suv39h1-KO CD8+ T cells, and day 7 p.i. Kb-OVA+ CD8+ T cells (from Listeria m.-OVA infected mice), were isolated by FACS. scRNA-seq was performed according to 10X Genomics technology. Both biological (Rep) and technical (tech) replicates were processed.
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| Contributor(s) |
Gueguen P, Bohec M, Baulande S, Goudot C, Amigorena S |
| Citation(s) |
29326266 |
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| Submission date |
Oct 27, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Luigia Pace |
| E-mail(s) |
Luigia.Pace@curie.fr
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| Organization name |
Institut Curie
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| Street address |
26 rue d'Ulm
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| City |
Paris |
| ZIP/Postal code |
75005 |
| Country |
France |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (8)
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| GSM2833287 |
kb-OVA+ CD8+ T cells WT, rep2, tech1 |
| GSM2833288 |
kb-OVA+ CD8+ T cells Suv39h1-KO, rep1, tech1 |
| GSM2833289 |
kb-OVA+ CD8+ T cells Suv39h1-KO, rep2 |
| GSM2833290 |
kb-OVA+ CD8+ T cells WT, rep2, tech2 |
| GSM2833291 |
kb-OVA+ CD8+ T cells Suv39h1-KO, rep1, tech2 |
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| This SubSeries is part of SuperSeries: |
| GSE106268 |
single-cell-RNA-sequencing and H3K9me3 occupancy of CD8+ T cells |
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| Relations |
| BioProject |
PRJNA416096 |
| SRA |
SRP122504 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE106264_RAW.tar |
20.8 Mb |
(http)(custom) |
TAR (of H5) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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