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| Status |
Public on Feb 06, 2019 |
| Title |
RNA-Seq profiling of 29 immune cell types and peripheral blood mononuclear cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We performed RNA-Seq transcriptome profiling on 29 immune cell types consituting peripheral blood mononuclear cells (PBMCs) sorted from 4 Singaporean-Chinese individuals (S4 cohort). We also performed RNA-Seq and microarray transcriptome profiling of PBMCs from an extended cohort of 13 individuals (S13 cohort). The data was used first to characterize the transcriptomic signatures and relationships among the 29 immune cell types. Then we explored the difference in mRNA composition in terms of transcripts proportions and abundance. Lastly, we performed deep deconvolution for both microarray and RNA-Seq technologies.
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| Overall design |
Total RNA of 29 immune cell types (from 4 individuals) and peripheral blood mononuclear cells (PBMCs, from 13 individuals) was extracted for gene expression profiling. The 13 PBMCs samples were processed with both microarray and RNA-Seq platforms.
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| Contributor(s) |
Monaco G, Lee B, Xu W, Hwang Y, Poidinger M, Poidinger M, de Magalhães J, Larbi A |
| Citation(s) |
30528453, 30726743 |
| |
| Submission date |
Nov 16, 2017 |
| Last update date |
Feb 05, 2023 |
| Contact name |
Gianni Monaco |
| E-mail(s) |
mongianni1@gmail.com
|
| Organization name |
Freiburg Medical Center
|
| Street address |
Breisacher Straße
|
| City |
Freiburg |
| ZIP/Postal code |
79106 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (127)
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| This SubSeries is part of SuperSeries: |
| GSE107019 |
Microarray and RNA-Seq profiling of immune cell types and peripheral blood mononuclear cells |
|
| Relations |
| BioProject |
PRJNA418779 |
| SRA |
SRP125125 |
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