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Series GSE107110 Query DataSets for GSE107110
Status Public on Jan 30, 2018
Title RNA-sequencing of bulk CD19+ Thymic B cells from young (5 week) and aged (10-24 month) mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Thymic B cells have been recently shown the ability to be licensed to express Aire, a critical transcription factor involved in the expression of self-antigens in the thymus which are critical for clonal deletion of autoreactive T cells and maintenance of self-tolerance. Mutations in AIRE cause systemic autoimmunity in humans and autoimmunity with varying degrees of severity in different mouse strains. Thymic B cells have been studied in young animals, but studies of this population with age are lacking. Given the thymus undergoes age-associated atrophy in its stromal compartment, we hypothesized that aged thymic B cells may under go changes with age which may impact their ability to mediate clonal deletion of autoreactive T cells and may contribute to age-associated increases in autoimmune prevalence. By comparing the transcriptome of young and aged thymic B cells we find that Aire and transcriptional activators of Aire are signficantly decreased with age.
Overall design Thymic B cells were enriched by incubating total thymocytes with CD19-APC (Clone: MB19-1) followed by incubation with anti-APC magnetic beads (Miltenyi Biotec). Enriched cells were then sorted using a BD FACS ARIA to a minimum purity of 98.5%, RNA extracted and analyzed
Contributor(s) Cepeda S, Cantu C, Orozco S, Xiao Y, Brown Z, Semwal MK, Venables T, Anderson MS, Griffith AV
Citation(s) 29386114
Submission date Nov 17, 2017
Last update date May 15, 2019
Contact name Yidong Chen
Phone 2105629163
Organization name UT Health Science Center at San Antonio
Department Population Health Sciences
Street address 8403 Floyd Curl Drive, MSC 7784
City San Antonio
State/province Texas
ZIP/Postal code 78229
Country USA
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (12)
GSM2861412 young female (2mo)
GSM2861413 1mo female
GSM2861414 4wo female #1
This SubSeries is part of SuperSeries:
GSE107112 RNA-sequencing of bulk CD19+ Thymic B cells from mice and humans
BioProject PRJNA419007
SRA SRP125234

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Supplementary file Size Download File type/resource
GSE107110_RAW.tar 1.5 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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