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Series GSE107124 Query DataSets for GSE107124
Status Public on Jul 26, 2018
Title Analysis of gene expression in populations of adult undifferentiated spermatogonia [RNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The undifferentiated spermatogonial population of mouse testis is known to be functionally heterogeneous and contain both stem cells and committed progenitor cells. However, gene expression patterns marking these distinct cell fractions are poorly defined. We found that a subset of undifferentiated spermatogonia were marked by expression of a PDX1-GFP transgene but properties of these cells were unclear. Undifferentiated cells were therefore isolated from adult testes and separated according to expression of PDX1-GFP+ for gene expression analysis by RNA-seq. Our goal was to identify differentially expressed genes from PDX1-GFP+ vs PDX1-GFP- with that of known markers of stem and committed progenitor cells.
 
Overall design 4 independent sets of PDX1-GFP-positive and PDX1-GFP-negative undifferentiated spermatogonia were isolated by flow sorting from adult mouse testes.
 
Contributor(s) Hobbs RM, La HM, Mäkelä J, Chan A, Rossello FJ, Nefzger CM, Legrand JM, De Seram M, Polo JM
Citation(s) 30026551
Submission date Nov 19, 2017
Last update date May 15, 2019
Contact name Robin Mark Hobbs
E-mail(s) robin.hobbs@monash.edu
Organization name Hudson Institute of Medical Research
Department Centre for Reproductive Health
Lab Germline Stem Cell Biology Laboratory
Street address 27-31 Wright Street
City CLAYTON
State/province VIC
ZIP/Postal code 3168
Country Australia
 
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (8)
GSM2861527 PDX1-GFPpos spermatogonia rep 1 [RNA-seq]
GSM2861528 PDX1-GFPneg spermatogonia rep 1 [RNA-seq]
GSM2861529 PDX1-GFPpos spermatogonia rep 2 [RNA-seq]
This SubSeries is part of SuperSeries:
GSE107256 Analysis of gene expression in populations of adult undifferentiated spermatogonia
Relations
BioProject PRJNA419077
SRA SRP125269

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE107124_NonStrandedCounts-withNames-proteinCoding.txt.gz 421.3 Kb (ftp)(http) TXT
GSE107124_NonStrandedCounts.summary.txt.gz 454 b (ftp)(http) TXT
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Processed data are available on Series record

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