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Series GSE107318 Query DataSets for GSE107318
Status Public on Nov 23, 2020
Title The global map of Rio1 activity in Saccharomyces cerevisiae
Organism Saccharomyces cerevisiae
Experiment type Genome variation profiling by genome tiling array
Summary In Saccharomyces cerevisiae, the kinase Rio1 regulates rDNA transcription and segregation, pre-rRNA cleavage, and 40S ribosomal subunit maturation. Other roles are unknown. Human orthologue RIOK1; which is frequently overexpressed in malignancies, drives tumor growth and metastasis. Again, also RIOK1 biology is poorly understood. In this study, we charted the global activity of Rio1 in budding yeast. By producing and systems-integrating its protein-interaction, gene-transcription, and chromatin-binding maps we generated Rio1's multi-layered activity network, which controls protein synthesis and turnover, metabolism, growth, proliferation, and genetic stability. Rio1 regulates itself at the transcriptional level, and manages its network both directly and indirectly, via a battery of regulators and transcription factors, including Gcn4. We experimentally confirmed the network and show that Rio1 commands its downstream circuit depending on the growth conditions encountered. We also find that Rio1 and RIOK1 activities are functionally equivalent. Our data suggest that pathological RIOK1 expression may deregulate its network and fuel promiscuous transcription and ribosome production, uncontrolled metabolism, growth, proliferation, and chromosomal instability; well-known contributors to cancer initiation, maintenance and metastasis.
 
Overall design ChIP-on-chip analyses were carried out as described in Bermejo R. et al., Meth. Mol. Biol. 582: 103-18 (2009). Labelled probes were hybridized to Affymetrix S.cerevisiae Tiling 1.0 (P/N 900645) arrays and processed with TAS software.
 
Contributor(s) De Wulf P
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Submission date Nov 24, 2017
Last update date Nov 24, 2020
Contact name Peter De Wulf
E-mail(s) peter.dewulf@ieo.eu
Phone +390294375036
Organization name European Institute of Oncology
Department Department of Experimental Oncology
Street address via Adamello 16
City Milan
ZIP/Postal code 20139
Country Italy
 
Platforms (1)
GPL7250 [Sc03b_MR] Affymetrix GeneChip S. cerevisiae Tiling 1.0R Array
Samples (2)
GSM2864770 RIO1
GSM2864771 6myc-RIO1
Relations
BioProject PRJNA419752

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE107318_RAW.tar 200.4 Mb (http)(custom) TAR (of BED, CEL)
Processed data provided as supplementary file

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