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Series GSE10768 Query DataSets for GSE10768
Status Public on May 08, 2008
Title Liver Genomic Responses to Ciguatoxin (P-CTX-1)
Organism Mus musculus
Experiment type Expression profiling by array
Summary Ciguatoxins (CTX) are polyether neurotoxins responsible for ciguatera, the most common fish-borne food poisoning in humans. This study characterizes the global transcriptional response of mouse liver to a symptomatic dose (0.26 ng/g) of the highly potent Pacific ciguatoxin-1 (P-CTX-1). At 1 h post exposure 2.4% of features on a 44K whole genome array were differentially expressed (p ≤ 0.0001), increasing to 5.2% at 4 h and decreasing to 1.4% by 24 h post-CTX exposure. Early gene expression was likely influenced prominently by an acute 4 °C decline in core body temperature by 1 h, which resolved by 8 h following exposure. Cytochrome P450s were of particular interest due to their role in xenobiotic metabolism. Twenty-seven genes, mostly members of Cyp2 and Cyp4 families, showed significant changes in expression. Many Cyps underwent an initial down-regulation at 1 h but were quickly and strongly up-regulated at 4 and 24 h post exposure. In addition to Cyps, increases in several glutathione S-transferases were observed, an indication that both phase I and phase II metabolic reactions are involved in the hepatic response to CTX in mice.
Keywords: biotoxin, time course
Overall design Individual C57/BL6 mice (n=3) exposed to 0.26 ng/g P-CTX-1 ip for 1, 4, or 24 h were compared to pooled time-matched controls (n=3). The triplicate arrays at each time point included a dye swap.
Contributor(s) Morey JS, Ryan JC, Van Dolah FM
Citation(s) 18353800
Submission date Mar 09, 2008
Last update date Feb 11, 2020
Contact name Frances M Van Dolah
Organization name Marine Biotoxins Program, NOAA Center for Coastal Environmental Health and Biomolecular Research
Street address 219 Fort Johnson Rd
City Charleston
State/province SC
ZIP/Postal code 29412
Country USA
Platforms (1)
GPL2872 Agilent-012694 Whole Mouse Genome G4122A (Feature Number version)
Samples (9)
GSM271869 Liver_Ciguatoxin (P-CTX-1)_1h_718+
GSM271870 Liver_Ciguatoxin (P-CTX-1)_1h_716-
GSM271871 Liver_Ciguatoxin (P-CTX-1)_1h_717+
BioProject PRJNA107559

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE10768_RAW.tar 6.2 Mb (http)(custom) TAR
Processed data included within Sample table

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