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Series GSE107893 Query DataSets for GSE107893
Status Public on Aug 24, 2018
Title Identification of Glucocorticoid-Induced Leucine Zipper (Gilz) gene targets in undifferentiated spermatogonia
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Sustained spermatogenesis in adult males and recovery of fertility following germ cell depletion are dependent on undifferentiated spermatogonia with self-renewal potential. We have previously demonstrated a critical cell-autonomous role for Gilz in spermatogonial stem cell maintainance and spermatogenesis. To identify genes regulated by Gilz in the male germline, we have isolated undifferentiated spermatogonial cells from tamoxifen treated Gilzflox/flox (Control) and Gilzflox/flox UBC-CreER (TAM-KO) mice that will allow identification of genes mis-expressed upon loss of GILZ.
Overall design 4 independent sets of Gilzflox/flox (Control) and Gilzflox/flox UBC-CreER (TAM-KO) undifferentiated spermatogonia were isolated by flow sorting from adult mouse testes 7 days after treatment with tamoxifen.
Contributor(s) Hobbs RM, La HM, Chan A, Legrand JM
Citation(s) 30126904
Submission date Dec 11, 2017
Last update date May 15, 2019
Contact name Robin Mark Hobbs
Organization name Hudson Institute of Medical Research
Department Centre for Reproductive Health
Lab Germline Stem Cell Biology Laboratory
Street address 27-31 Wright Street
State/province VIC
ZIP/Postal code 3168
Country Australia
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (8)
GSM2882955 Gilzflox/flox (Control) spermatogonia rep 1
GSM2882956 Gilzflox/flox UBC-CreER (TAM-KO) spermatogonia rep 1
GSM2882957 Gilzflox/flox (Control) spermatogonia rep 2
BioProject PRJNA421886
SRA SRP126482

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Supplementary file Size Download File type/resource
GSE107893_NonStrandedCounts-withNames-proteinCoding.txt.gz 426.2 Kb (ftp)(http) TXT
GSE107893_NonStrandedCounts.summary.txt.gz 483 b (ftp)(http) TXT
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