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Series GSE107904 Query DataSets for GSE107904
Status Public on Jan 15, 2018
Title Oryza sativa Argonaute 18 is a regulator of male sterility (sRNA-Seq)
Organism Oryza sativa
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Artificial miRNA mediated knock-down ago18 transgenic lines [ago18(1), ago18(2)] showed developmentally compromised phenotype in both vegetative and reproductive tissues compared to wild-type control. We have performed small RNA seq to investigate underlying molecular mechanism and to reveal functional role of AGO18 in rice.
 
Overall design We have performed small RNA seq analysis using vegetative and reproductive tissues of both WT and knock-down tansgenic lines. Four weeks old seedlings were considered as vegetative tissues and two different stage of panicle were examined as reprodictive tissues. Libraries were prepared from total RNA isolated from the tissues mentioned.
 
Contributor(s) Shivaprasad PV, Chenna S, Das S
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Submission date Dec 11, 2017
Last update date May 15, 2019
Contact name Shivaprasad Padubidri V.
E-mail(s) shivaprasad@ncbs.res.in
Phone +91 80 2366 6511
Organization name National Center for Biological Sciences
Department Plant epigenetics
Street address GKVK Campus, Bellary road
City Bangalore
ZIP/Postal code 560065
Country India
 
Platforms (1)
GPL21087 Illumina NextSeq 500 (Oryza sativa)
Samples (18)
GSM2883136 WT_seedling_Rep_1
GSM2883137 WT_seedling_Rep_2
GSM2883138 WT_young_panicle_Rep_1
Relations
BioProject PRJNA421904
SRA SRP126493

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE107904_RAW.tar 258.2 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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