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Series GSE108136 Query DataSets for GSE108136
Status Public on Dec 16, 2017
Title Snail-dependent epithelial splicing regulatory protein 1 (ESRP1) silencing drives malignant transformation of human pulmonary epithelial cells [miRNA]
Platform organisms Homo sapiens; Mus musculus; Rattus norvegicus; Human alphaherpesvirus 1; Human betaherpesvirus 5; Murid betaherpesvirus 1; human gammaherpesvirus 4; JC polyomavirus; Human immunodeficiency virus 1; Murid gammaherpesvirus 4; Human gammaherpesvirus 8; Betapolyomavirus hominis; Betapolyomavirus macacae
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary The significance of epithelial-to-mesenchymal transition (EMT)-inducing transcription factors in the onset of non-small cell lung cancer has not been resolved. Here, we report increased Snail expression in pulmonary premalignant lesions relative to histologically normal-appearing pulmonary epithelium. Utilizing immortalized human pulmonary epithelial cells and isogenic derivatives, we document Snail-dependent anchorage-independent growth of the epithelial cells in vitro, as well as transformation, primary tumor growth, and metastatic behavior in vivo. Epithelial splicing regulatory protein 1 (ESRP1) tumor suppressor silencing was a requirement for Snail-driven transformation in vivo, and we identified ESRP1 loss in Snail-expressing pulmonary premalignant lesions in situ. Snail drives these and other carcinogenic signaling programs in an ALDH+CD44+CD24- pulmonary stem cell subset in which ESRP1 and stemness-repressing micro-RNAs are inhibited.
Overall design Two distinct HBEC cell lines were transduced with a retroviral vector containing human SNAI1 (Snail) cDNA or the empty vector. Their micro RNAs were profiled to determine the impact of ectopic Snail expression on the isogenic epithelial background of the HBECs.
Contributor(s) Walser TC, Jing Z, Tran LM, Lin Y, Yakobian N, Wang G, Krysan K, Zhu LX, Sharma S, Lee M, Belperio JA, Ooi A, Gomperts BN, Shay JW, Larsen JE, Minna JD, Hong L, Fishbein MC, Dubinett SM
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Submission date Dec 15, 2017
Last update date Jan 24, 2018
Contact name Linh My Tran
Organization name University of Los Angeles
Department Medicine - Pulmonary & Critical Care
Lab Dubinett Lab
Street address Box 951690, 37-131 CHS
City Los Angeles
State/province California
ZIP/Postal code 90095-1690
Country USA
Platforms (1)
GPL7723 miRCURY LNA microRNA Array, v.11.0 - hsa, mmu & rno
Samples (4)
GSM2891007 HBEC3_vector_miRNA
GSM2891008 HBEC3_SnailOEr_miRNA
GSM2891009 HBEC4_vector_miRNA
This SubSeries is part of SuperSeries:
GSE108137 Snail-dependent epithelial splicing regulatory protein 1 (ESRP1) silencing drives malignant transformation of human pulmonary epithelial cells
BioProject PRJNA422652

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE108136_TCW_miRNA_Snail_raw2.txt.gz 163.1 Kb (ftp)(http) TXT
Processed data included within Sample table

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