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Series GSE108141 Query DataSets for GSE108141
Status Public on Dec 15, 2017
Title Morphological and transcriptomic effects of endocrine modulators on the gonadal differentiation of chicken embryos: the case of tributyltin (TBT)
Organism Gallus gallus
Experiment type Expression profiling by high throughput sequencing
Summary Chicken eggs (Gallus gallus domesticus) were incubated, after 24 h of incubation either non-injected (control, C), or injected with the solvent sesame oil (Oleum Sesame Raffinatum, solvent control, SC), and the substances Tributyltin (TBT, 10pg TBT-SN/g egg) or 17alpha-Methyltestosteron (MT, 30 pg/g egg).
Animals were decapitated on breeding day 19, 2 days before anticipated hatching. Right and left gonads of female and male chicken were accurately separated from adhesing tissues, stored in RNA lysis buffer (Promega) at -80°C until until RNA isolation with the SV Total RNA Isolation System Kit according to manual 048 (Promega).
Sequencing was performed on Illumina’s Genome Analyzer IIx, and subsequent base calling was carried out by Illumina’s GAPipeline.
Our study represents the first detailed analysis of whole chicken gonad transcriptomes, with biologic replicates, generated by SuperSAGE technology.
Overall design SuperSAGE analysis of substance-induced whole-gemome expression in 19-days-bred chicken gonads
Contributor(s) Scheider J, Afonso-Grunz F
Citation(s) 29191790
Submission date Dec 15, 2017
Last update date Jan 24, 2018
Contact name GenXPro GenXPro
Organization name GenXPro GmbH
Street address Altenhöferallee 3
City Frankfurt am Main
ZIP/Postal code 60438
Country Germany
Platforms (1)
GPL13797 Illumina Genome Analyzer IIx (Gallus gallus)
Samples (16)
GSM2891097 FGR_C
GSM2891098 FGL_C
GSM2891099 MGR_C
BioProject PRJNA422574
SRA SRP126846

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Supplementary file Size Download File type/resource
GSE108141_supplementary_tab_T1.xlsx 4.8 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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