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Series GSE108315 Query DataSets for GSE108315
Status Public on May 14, 2018
Title De novo formation of a functional biliary system by TGFβ-mediated hepatocyte transdifferentiation
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We observed de novo formation of peripheral bile ducts by transdifferentiation of hepatocytes in mice born without peripheral bile ducts. To assess the authenticity and maturity of the hepatocyte-derived peripheral cholangiocytes forming the new bile ducts, we compared their global gene expression profile to that of peripheral cholangiocytes isolated from normal mice. We also included hepatocytes isolated from mice born without peripheral bile ducts as a control.
Overall design We isolated hepatocyte-derived peripheral cholangiocytes, peripheral cholangiocytes and hepatocytes from mouse livers by FACS, extracted RNA and performed RNA-seq, using single end 50bp reads.
Contributor(s) Willenbring H, Schaub JR, Kurial SN, Karns RA, Huppert SS
Citation(s) 29720662
Submission date Dec 19, 2017
Last update date Feb 11, 2019
Contact name Rebekah Karns
Organization name Cincinnati Children's Hospital Medical Center
Department Gastroenterology, Hepatology, & Nutrition
Street address 3333 Burnet Ave
City Cincinnati
State/province OH
ZIP/Postal code 45276
Country USA
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (10)
GSM2895299 HpC1
GSM2895300 HpC2
GSM2895301 HpC3
BioProject PRJNA423097
SRA SRP127184

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE108315_RAW.tar 5.0 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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