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Series GSE108688 Query DataSets for GSE108688
Status Public on Oct 22, 2019
Title GR cistromes reprogramming by High Fat Diet [RNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We mapped the genome-wide binding profiles of GR throughout the day/night cycle (ZT0-ZT4-ZT8-ZT12-ZT16-ZT20) by using ChIP-Seq in livers from control mice and mice fed High Fat Diet (HFD) for 12 weeks
Overall design Male C57BL/6J mice were fed either control diet (11 kcal% fat w/sucrose, Research Diets D12329) or High Fat Diet (58 kcal% fat w/sucrose, Research Diets D12331) for 12 weeks. At 17 weeks of age, mice were sacrificed by cervical dislocation, and livers were collected at the indicated 4h intervals (ZT0,4,8,12,16,20). Dexamethasone (Sigma, D2915) was administered at ZT0 and at ZT12 as a single i.p. injection of 10mg/kg after 12 weeks of high fat or control diet. Mice were sacrificed by cervical dislocation 4h later. Hepatocyte-specific Alb-Cre x GRflox/flox (Opherk et al. 2004) and controls were placed on HFD or control diet for 12 weeks. Livers were collected after cervical dislocation at the indicated 4h intervals (ZT0,4,8,12,16,20).
Contributor(s) Balázs K, Quagliarini F
Citation(s) 31706703
Submission date Jan 03, 2018
Last update date Jan 21, 2020
Contact name Nina Henriette Uhlenhaut
Organization name HelmholtzZentrum München - IDC
Department Molecular Endocrinology
Lab Uhlenhaut IDC
Street address Ingolstädter Landstraße 1, Neuherberg
City Munich
State/province Bavaria
ZIP/Postal code D-85764
Country Germany
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (122)
GSM2911206 RNA-seq_ZT0 control REP1
GSM2911207 RNA-seq_ZT0 control REP2
GSM2911208 RNA-seq_ZT0 control REP3
This SubSeries is part of SuperSeries:
GSE108690 Cistromic reprogramming of the diurnal glucocorticoid hormone response by high fat diet
BioProject PRJNA428303
SRA SRP127946

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE108688_table_featureCounts.txt.gz 1.3 Mb (ftp)(http) TXT
GSE108688_table_featureCounts_KO-WT.txt.gz 369.9 Kb (ftp)(http) TXT
GSE108688_table_featureCounts_KO-WT_AlbCre.txt.gz 1.9 Mb (ftp)(http) TXT
GSE108688_table_featureCounts_ZT04.txt.gz 496.7 Kb (ftp)(http) TXT
GSE108688_table_featureCounts_ZT1216.txt.gz 498.6 Kb (ftp)(http) TXT
GSE108688_table_featureCounts_noBL41.txt.gz 1.2 Mb (ftp)(http) TXT
GSE108688_table_filtered_KO-WT_AlbCre_VST.txt.gz 4.9 Mb (ftp)(http) TXT
GSE108688_table_filtered_KO-WT_AlbCre_rlog.txt.gz 4.9 Mb (ftp)(http) TXT
GSE108688_table_filtered_VST.txt.gz 3.2 Mb (ftp)(http) TXT
GSE108688_table_filtered_VST_KO-WT.txt.gz 572.9 Kb (ftp)(http) TXT
GSE108688_table_filtered_VST_ZT04.txt.gz 912.6 Kb (ftp)(http) TXT
GSE108688_table_filtered_VST_ZT1216.txt.gz 926.6 Kb (ftp)(http) TXT
GSE108688_table_filtered_VST_noBL41.txt.gz 3.0 Mb (ftp)(http) TXT
GSE108688_table_filtered_noBL41_VST.txt.gz 3.0 Mb (ftp)(http) TXT
GSE108688_table_filtered_noBL41_rlog.txt.gz 6.8 Mb (ftp)(http) TXT
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