Expression profiling by high throughput sequencing
Summary
We mapped the genome-wide binding profiles of GR throughout the day/night cycle (ZT0-ZT4-ZT8-ZT12-ZT16-ZT20) by using ChIP-Seq in livers from control mice and mice fed High Fat Diet (HFD) for 12 weeks
Overall design
Male C57BL/6J mice were fed either control diet (11 kcal% fat w/sucrose, Research Diets D12329) or High Fat Diet (58 kcal% fat w/sucrose, Research Diets D12331) for 12 weeks. At 17 weeks of age, mice were sacrificed by cervical dislocation, and livers were collected at the indicated 4h intervals (ZT0,4,8,12,16,20). Dexamethasone (Sigma, D2915) was administered at ZT0 and at ZT12 as a single i.p. injection of 10mg/kg after 12 weeks of high fat or control diet. Mice were sacrificed by cervical dislocation 4h later. Hepatocyte-specific Alb-Cre x GRflox/flox (Opherk et al. 2004) and controls were placed on HFD or control diet for 12 weeks. Livers were collected after cervical dislocation at the indicated 4h intervals (ZT0,4,8,12,16,20).
Less...
More...