GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE108774 Query DataSets for GSE108774
Status Public on Oct 16, 2019
Title Distinct Roles for Condensin's Two ATPase Sites in Chromosome Condensation.
Organism Homo sapiens
Experiment type Other
Summary Condensin is a conserved SMC complex that uses its ATPase machinery to structure genomes, but how it does so is largely unknown. We show that condensin’s ATPase has a dual role in chromosome condensation. Mutation of one ATPase site impairs condensation, while mutating the second site results in hyperactive condensin that compacts DNA faster than wild type, both in vivo and in vitro. Whereas one site drives loop formation, the second site is involved in the formation of more stable higher-order Z loop structures. Using hyperactive condensin I, we reveal that condensin II is not intrinsically needed for the shortening of mitotic chromosomes. Condensin II rather is required for a straight chromosomal axis and enables faithful chromosome segregation by counteracting the formation of ultrafine DNA bridges. SMC complexes with distinct roles for each ATPase site likely reflect a universal principle that enables these molecular machines to intricately control chromosome architecture.
Overall design Hi-C was performed in control, Caph2KO, AS2LV, AS1LV, AS2LV/LV, SMC2WT/∆ and CAPH2KO-AS2LV cell lines in technical replicates.
Contributor(s) van der Weide RH, de Wit E
Citation(s) 31629658
Submission date Jan 04, 2018
Last update date Jan 16, 2020
Contact name Robin H. van der Weide
Organization name Hubrecht Institute
Lab Kind Lab
Street address Uppsalalaan 8
City Utrecht
State/province Utrecht
ZIP/Postal code 3584 CT
Country Netherlands
Platforms (1)
GPL20795 HiSeq X Ten (Homo sapiens)
Samples (7)
GSM2913118 Hap1-A Hi-C
GSM2913119 Hap1-CAPH2KO
GSM2913120 Hap1-AS2LV
BioProject PRJNA428522
SRA SRP130935

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE108774_RAW.tar 7.5 Gb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap